Efficient nested-PCR-based method development for detection and genotype identification of Acanthamoeba from a small volume of aquatic environmental sample

Abstract Acanthamoeba spp. are opportunistic human pathogens that cause granulomatous amoebic encephalitis and keratitis, and their accurate detection and enumeration in environmental samples is a challenge. In addition, information regarding the genotyping of Acanthamoeba spp. using various PCR met...

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Main Authors: Tsui-Kang Hsu, Jung-Sheng Chen, Hsin-Chi Tsai, Chi-Wei Tao, Yu-Yin Yang, Ying-Chin Tseng, Yi-Jie Kuo, Dar-Der Ji, Jagat Rathod, Bing-Mu Hsu
Format: article
Language:EN
Published: Nature Portfolio 2021
Subjects:
R
Q
Online Access:https://doaj.org/article/dfd24566f462408788bb3571f4df0433
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