A Three-Dimensional Printed Polycaprolactone Scaffold Combined with Co-Axially Electrospun Vancomycin/Ceftazidime/Bone Morphological Protein-2 Sheath-Core Nanofibers for the Repair of Segmental Bone Defects During the Masquelet Procedure

Yi-Hsun Yu,1,2 Demei Lee,2 Yung-Heng Hsu,1,2 Ying-Chao Chou,1,2 Steve WN Ueng,1 Che-Kang Chen,2 Shih-Jung Liu1,2 1Department of Orthopedic Surgery, Musculoskeletal Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan; 2Department of Mechanical Engineering, Chang Gung University, Taoyuan, T...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yu YH, Lee D, Hsu YH, Chou YC, Ueng SWN, Chen CK, Liu SJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	masquelet procedure composite scaffold three-dimensional printing co-axial electrospinning Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/dfd3b4a1df3f447da61224b2bcef9ef1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dfd3b4a1df3f447da61224b2bcef9ef1
record_format	dspace
spelling	oai:doaj.org-article:dfd3b4a1df3f447da61224b2bcef9ef12021-12-02T07:19:38ZA Three-Dimensional Printed Polycaprolactone Scaffold Combined with Co-Axially Electrospun Vancomycin/Ceftazidime/Bone Morphological Protein-2 Sheath-Core Nanofibers for the Repair of Segmental Bone Defects During the Masquelet Procedure1178-2013https://doaj.org/article/dfd3b4a1df3f447da61224b2bcef9ef12020-02-01T00:00:00Zhttps://www.dovepress.com/a-three-dimensional-printed-polycaprolactone-scaffold-combined-with-co-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yi-Hsun Yu,1,2 Demei Lee,2 Yung-Heng Hsu,1,2 Ying-Chao Chou,1,2 Steve WN Ueng,1 Che-Kang Chen,2 Shih-Jung Liu1,2 1Department of Orthopedic Surgery, Musculoskeletal Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan; 2Department of Mechanical Engineering, Chang Gung University, Taoyuan, TaiwanCorrespondence: Shih-Jung LiuBiomaterials Lab, Department of Mechanical Engineering, Chang Gung University, No. 259 Wen-Hwa 1st Road, Guishan District, Taoyuan 333, TaiwanTel +886 3 211 8166Fax +886 3 211 8558Email shihjung@mail.cgu.edu.twIntroduction: Masquelet proposed a new solution for the healing of segmental bone defects, thus minimizing the disadvantages associated with traditional bone grafting. However, a major factor leading to the failure of this technique pertains to be the residual infection. Accordingly, we developed an antibiotic- and osteo-inductive agent-loaded composite scaffold to solve this problem.Methods: A mesh-like polycaprolactone scaffold was prepared using a lab-exploited solution-type three-dimensional printer, and hybrid sheath-core structured poly(lactic-co-glycolic-acid) nanofibers were fabricated using co-axial electrospinning technology. Vancomycin, ceftazidime, and bone morphological protein (BMP)-2 were employed. The in vitro and in vivo (rabbit fracture model) release patterns of applied agents from the composite scaffold were investigated.Results: The results revealed that the drug-eluting composite scaffold enabled the sustainable release of the medications for at least 30 days in vitro. Animal tests demonstrated that a high concentration of medications was maintained. Abundant growth factors were induced within the bioactive membrane stimulated by the applied scaffold. Finally, satisfactory bone healing potential was observed on radiological examination and biomechanical evaluation.Discussion: The developed composite scaffold may facilitate bone healing by inducing bioactive membrane formation and yielding high concentrations of antibiotics and BMP-2 during the Masquelet procedure.Keywords: Masquelet procedure, composite scaffold, three-dimensional printing, co-axial electrospinningYu YHLee DHsu YHChou YCUeng SWNChen CKLiu SJDove Medical Pressarticlemasquelet procedurecomposite scaffoldthree-dimensional printingco-axial electrospinningMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 913-925 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	masquelet procedure composite scaffold three-dimensional printing co-axial electrospinning Medicine (General) R5-920
spellingShingle	masquelet procedure composite scaffold three-dimensional printing co-axial electrospinning Medicine (General) R5-920 Yu YH Lee D Hsu YH Chou YC Ueng SWN Chen CK Liu SJ A Three-Dimensional Printed Polycaprolactone Scaffold Combined with Co-Axially Electrospun Vancomycin/Ceftazidime/Bone Morphological Protein-2 Sheath-Core Nanofibers for the Repair of Segmental Bone Defects During the Masquelet Procedure
description	Yi-Hsun Yu,1,2 Demei Lee,2 Yung-Heng Hsu,1,2 Ying-Chao Chou,1,2 Steve WN Ueng,1 Che-Kang Chen,2 Shih-Jung Liu1,2 1Department of Orthopedic Surgery, Musculoskeletal Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan; 2Department of Mechanical Engineering, Chang Gung University, Taoyuan, TaiwanCorrespondence: Shih-Jung LiuBiomaterials Lab, Department of Mechanical Engineering, Chang Gung University, No. 259 Wen-Hwa 1st Road, Guishan District, Taoyuan 333, TaiwanTel +886 3 211 8166Fax +886 3 211 8558Email shihjung@mail.cgu.edu.twIntroduction: Masquelet proposed a new solution for the healing of segmental bone defects, thus minimizing the disadvantages associated with traditional bone grafting. However, a major factor leading to the failure of this technique pertains to be the residual infection. Accordingly, we developed an antibiotic- and osteo-inductive agent-loaded composite scaffold to solve this problem.Methods: A mesh-like polycaprolactone scaffold was prepared using a lab-exploited solution-type three-dimensional printer, and hybrid sheath-core structured poly(lactic-co-glycolic-acid) nanofibers were fabricated using co-axial electrospinning technology. Vancomycin, ceftazidime, and bone morphological protein (BMP)-2 were employed. The in vitro and in vivo (rabbit fracture model) release patterns of applied agents from the composite scaffold were investigated.Results: The results revealed that the drug-eluting composite scaffold enabled the sustainable release of the medications for at least 30 days in vitro. Animal tests demonstrated that a high concentration of medications was maintained. Abundant growth factors were induced within the bioactive membrane stimulated by the applied scaffold. Finally, satisfactory bone healing potential was observed on radiological examination and biomechanical evaluation.Discussion: The developed composite scaffold may facilitate bone healing by inducing bioactive membrane formation and yielding high concentrations of antibiotics and BMP-2 during the Masquelet procedure.Keywords: Masquelet procedure, composite scaffold, three-dimensional printing, co-axial electrospinning
format	article
author	Yu YH Lee D Hsu YH Chou YC Ueng SWN Chen CK Liu SJ
author_facet	Yu YH Lee D Hsu YH Chou YC Ueng SWN Chen CK Liu SJ
author_sort	Yu YH
title	A Three-Dimensional Printed Polycaprolactone Scaffold Combined with Co-Axially Electrospun Vancomycin/Ceftazidime/Bone Morphological Protein-2 Sheath-Core Nanofibers for the Repair of Segmental Bone Defects During the Masquelet Procedure
title_short	A Three-Dimensional Printed Polycaprolactone Scaffold Combined with Co-Axially Electrospun Vancomycin/Ceftazidime/Bone Morphological Protein-2 Sheath-Core Nanofibers for the Repair of Segmental Bone Defects During the Masquelet Procedure
title_full	A Three-Dimensional Printed Polycaprolactone Scaffold Combined with Co-Axially Electrospun Vancomycin/Ceftazidime/Bone Morphological Protein-2 Sheath-Core Nanofibers for the Repair of Segmental Bone Defects During the Masquelet Procedure
title_fullStr	A Three-Dimensional Printed Polycaprolactone Scaffold Combined with Co-Axially Electrospun Vancomycin/Ceftazidime/Bone Morphological Protein-2 Sheath-Core Nanofibers for the Repair of Segmental Bone Defects During the Masquelet Procedure
title_full_unstemmed	A Three-Dimensional Printed Polycaprolactone Scaffold Combined with Co-Axially Electrospun Vancomycin/Ceftazidime/Bone Morphological Protein-2 Sheath-Core Nanofibers for the Repair of Segmental Bone Defects During the Masquelet Procedure
title_sort	three-dimensional printed polycaprolactone scaffold combined with co-axially electrospun vancomycin/ceftazidime/bone morphological protein-2 sheath-core nanofibers for the repair of segmental bone defects during the masquelet procedure
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/dfd3b4a1df3f447da61224b2bcef9ef1
work_keys_str_mv	AT yuyh athreedimensionalprintedpolycaprolactonescaffoldcombinedwithcoaxiallyelectrospunvancomycinceftazidimebonemorphologicalprotein2sheathcorenanofibersfortherepairofsegmentalbonedefectsduringthemasqueletprocedure AT leed athreedimensionalprintedpolycaprolactonescaffoldcombinedwithcoaxiallyelectrospunvancomycinceftazidimebonemorphologicalprotein2sheathcorenanofibersfortherepairofsegmentalbonedefectsduringthemasqueletprocedure AT hsuyh athreedimensionalprintedpolycaprolactonescaffoldcombinedwithcoaxiallyelectrospunvancomycinceftazidimebonemorphologicalprotein2sheathcorenanofibersfortherepairofsegmentalbonedefectsduringthemasqueletprocedure AT chouyc athreedimensionalprintedpolycaprolactonescaffoldcombinedwithcoaxiallyelectrospunvancomycinceftazidimebonemorphologicalprotein2sheathcorenanofibersfortherepairofsegmentalbonedefectsduringthemasqueletprocedure AT uengswn athreedimensionalprintedpolycaprolactonescaffoldcombinedwithcoaxiallyelectrospunvancomycinceftazidimebonemorphologicalprotein2sheathcorenanofibersfortherepairofsegmentalbonedefectsduringthemasqueletprocedure AT chenck athreedimensionalprintedpolycaprolactonescaffoldcombinedwithcoaxiallyelectrospunvancomycinceftazidimebonemorphologicalprotein2sheathcorenanofibersfortherepairofsegmentalbonedefectsduringthemasqueletprocedure AT liusj athreedimensionalprintedpolycaprolactonescaffoldcombinedwithcoaxiallyelectrospunvancomycinceftazidimebonemorphologicalprotein2sheathcorenanofibersfortherepairofsegmentalbonedefectsduringthemasqueletprocedure AT yuyh threedimensionalprintedpolycaprolactonescaffoldcombinedwithcoaxiallyelectrospunvancomycinceftazidimebonemorphologicalprotein2sheathcorenanofibersfortherepairofsegmentalbonedefectsduringthemasqueletprocedure AT leed threedimensionalprintedpolycaprolactonescaffoldcombinedwithcoaxiallyelectrospunvancomycinceftazidimebonemorphologicalprotein2sheathcorenanofibersfortherepairofsegmentalbonedefectsduringthemasqueletprocedure AT hsuyh threedimensionalprintedpolycaprolactonescaffoldcombinedwithcoaxiallyelectrospunvancomycinceftazidimebonemorphologicalprotein2sheathcorenanofibersfortherepairofsegmentalbonedefectsduringthemasqueletprocedure AT chouyc threedimensionalprintedpolycaprolactonescaffoldcombinedwithcoaxiallyelectrospunvancomycinceftazidimebonemorphologicalprotein2sheathcorenanofibersfortherepairofsegmentalbonedefectsduringthemasqueletprocedure AT uengswn threedimensionalprintedpolycaprolactonescaffoldcombinedwithcoaxiallyelectrospunvancomycinceftazidimebonemorphologicalprotein2sheathcorenanofibersfortherepairofsegmentalbonedefectsduringthemasqueletprocedure AT chenck threedimensionalprintedpolycaprolactonescaffoldcombinedwithcoaxiallyelectrospunvancomycinceftazidimebonemorphologicalprotein2sheathcorenanofibersfortherepairofsegmentalbonedefectsduringthemasqueletprocedure AT liusj threedimensionalprintedpolycaprolactonescaffoldcombinedwithcoaxiallyelectrospunvancomycinceftazidimebonemorphologicalprotein2sheathcorenanofibersfortherepairofsegmentalbonedefectsduringthemasqueletprocedure
_version_	1718399487243714560

A Three-Dimensional Printed Polycaprolactone Scaffold Combined with Co-Axially Electrospun Vancomycin/Ceftazidime/Bone Morphological Protein-2 Sheath-Core Nanofibers for the Repair of Segmental Bone Defects During the Masquelet Procedure

Ejemplares similares