Visible colorimetric growth indicators of Neisseria gonorrhoeae for low-cost diagnostic applications.

N. gonorrhoeae is one of the most pressing antibiotic resistant threats of our time and low-cost diagnostics that can easily identify antibiotic resistance are desperately needed. However, N. gonorrhoeae responds so uniquely to growth conditions that it cannot be assumed gonorrhea will respond to co...

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Autores principales:	Taylor Mae Oeschger, David Carl Erickson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dfd40ea550b64652a4e039fe245cba13
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spelling	oai:doaj.org-article:dfd40ea550b64652a4e039fe245cba132021-12-02T20:10:28ZVisible colorimetric growth indicators of Neisseria gonorrhoeae for low-cost diagnostic applications.1932-620310.1371/journal.pone.0252961https://doaj.org/article/dfd40ea550b64652a4e039fe245cba132021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252961https://doaj.org/toc/1932-6203N. gonorrhoeae is one of the most pressing antibiotic resistant threats of our time and low-cost diagnostics that can easily identify antibiotic resistance are desperately needed. However, N. gonorrhoeae responds so uniquely to growth conditions that it cannot be assumed gonorrhea will respond to common microbiological methods used for other pathogenic organisms. In this paper, we explore visual colorimetric indicators of N. gonorrhoeae growth that can be seen without a microscope or spectrophotometer. We evaluate growth media, pH indicators, resazurin-based dyes, and tetrazolium-based dyes for their use in simple colorimetric system. Overall, we identified Graver Wade media as the best at supporting robust gonococcal growth while also providing the least background when analyzing results of colorimetric tests. XTT, a tetrazolium-based dye, proved to show to brightest color change over time and not negatively impact the natural growth of N. gonorrhoeae. However, other dyes including PrestoBlue, MTT, and NBT are less expensive than XTT and work well when added after bacterial growth has already occurred. By identifying the specific use cases of these dyes, this research lays the groundwork for future development of a color-based antibiotic susceptibility low-cost test for N. gonorrhoeae.Taylor Mae OeschgerDavid Carl EricksonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252961 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Taylor Mae Oeschger David Carl Erickson Visible colorimetric growth indicators of Neisseria gonorrhoeae for low-cost diagnostic applications.
description	N. gonorrhoeae is one of the most pressing antibiotic resistant threats of our time and low-cost diagnostics that can easily identify antibiotic resistance are desperately needed. However, N. gonorrhoeae responds so uniquely to growth conditions that it cannot be assumed gonorrhea will respond to common microbiological methods used for other pathogenic organisms. In this paper, we explore visual colorimetric indicators of N. gonorrhoeae growth that can be seen without a microscope or spectrophotometer. We evaluate growth media, pH indicators, resazurin-based dyes, and tetrazolium-based dyes for their use in simple colorimetric system. Overall, we identified Graver Wade media as the best at supporting robust gonococcal growth while also providing the least background when analyzing results of colorimetric tests. XTT, a tetrazolium-based dye, proved to show to brightest color change over time and not negatively impact the natural growth of N. gonorrhoeae. However, other dyes including PrestoBlue, MTT, and NBT are less expensive than XTT and work well when added after bacterial growth has already occurred. By identifying the specific use cases of these dyes, this research lays the groundwork for future development of a color-based antibiotic susceptibility low-cost test for N. gonorrhoeae.
format	article
author	Taylor Mae Oeschger David Carl Erickson
author_facet	Taylor Mae Oeschger David Carl Erickson
author_sort	Taylor Mae Oeschger
title	Visible colorimetric growth indicators of Neisseria gonorrhoeae for low-cost diagnostic applications.
title_short	Visible colorimetric growth indicators of Neisseria gonorrhoeae for low-cost diagnostic applications.
title_full	Visible colorimetric growth indicators of Neisseria gonorrhoeae for low-cost diagnostic applications.
title_fullStr	Visible colorimetric growth indicators of Neisseria gonorrhoeae for low-cost diagnostic applications.
title_full_unstemmed	Visible colorimetric growth indicators of Neisseria gonorrhoeae for low-cost diagnostic applications.
title_sort	visible colorimetric growth indicators of neisseria gonorrhoeae for low-cost diagnostic applications.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/dfd40ea550b64652a4e039fe245cba13
work_keys_str_mv	AT taylormaeoeschger visiblecolorimetricgrowthindicatorsofneisseriagonorrhoeaeforlowcostdiagnosticapplications AT davidcarlerickson visiblecolorimetricgrowthindicatorsofneisseriagonorrhoeaeforlowcostdiagnosticapplications
_version_	1718375030245556224

Visible colorimetric growth indicators of Neisseria gonorrhoeae for low-cost diagnostic applications.

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