Strain-Specific Liver Metabolite Profiles in Medaka

The relationship between genetic variation and phenotypic traits is often poorly understood since specific genotypes do not always easily translate into associated phenotypes, especially for complex disorders. The genetic background has been shown to affect metabolic pathways and thus contribute to...

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Autores principales:	Hannah Soergel, Felix Loosli, Claudia Muhle-Goll
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	liver multivariate analysis NMR spectroscopy <i>Oryzias latipes</i> univariate analysis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/dfe0577cb3744334b033397934db2761
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id	oai:doaj.org-article:dfe0577cb3744334b033397934db2761
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spelling	oai:doaj.org-article:dfe0577cb3744334b033397934db27612021-11-25T18:20:36ZStrain-Specific Liver Metabolite Profiles in Medaka10.3390/metabo111107442218-1989https://doaj.org/article/dfe0577cb3744334b033397934db27612021-10-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/744https://doaj.org/toc/2218-1989The relationship between genetic variation and phenotypic traits is often poorly understood since specific genotypes do not always easily translate into associated phenotypes, especially for complex disorders. The genetic background has been shown to affect metabolic pathways and thus contribute to variations in the metabolome. Here, we tested the suitability of NMR metabolomics for comparative analysis of fish lines as a first step towards phenotype-genotype association studies. The Japanese rice fish, medaka (<i>Oryzias latipes</i>), is a widely used genetic vertebrate model with several isogenic inbred laboratory strains. We used liver extracts of medaka iCab and HO5 strains as a paradigm to test the feasibility of distinguishing the metabolome of two different inbred strains. Fifteen metabolites could be detected in uni- and multivariate analyses that showed strain-specific levels. Differences could be assigned to specific metabolic pathways. Our results show that NMR spectroscopy is a suitable method to detect variance of the metabolome caused by subtle genetic differences. Thus, it has the potential to address genotype–phenotype associations in medaka, providing an additional level of phenotypic analysis.Hannah SoergelFelix LoosliClaudia Muhle-GollMDPI AGarticlelivermultivariate analysisNMR spectroscopy<i>Oryzias latipes</i>univariate analysisMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 744, p 744 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	liver multivariate analysis NMR spectroscopy <i>Oryzias latipes</i> univariate analysis Microbiology QR1-502
spellingShingle	liver multivariate analysis NMR spectroscopy <i>Oryzias latipes</i> univariate analysis Microbiology QR1-502 Hannah Soergel Felix Loosli Claudia Muhle-Goll Strain-Specific Liver Metabolite Profiles in Medaka
description	The relationship between genetic variation and phenotypic traits is often poorly understood since specific genotypes do not always easily translate into associated phenotypes, especially for complex disorders. The genetic background has been shown to affect metabolic pathways and thus contribute to variations in the metabolome. Here, we tested the suitability of NMR metabolomics for comparative analysis of fish lines as a first step towards phenotype-genotype association studies. The Japanese rice fish, medaka (<i>Oryzias latipes</i>), is a widely used genetic vertebrate model with several isogenic inbred laboratory strains. We used liver extracts of medaka iCab and HO5 strains as a paradigm to test the feasibility of distinguishing the metabolome of two different inbred strains. Fifteen metabolites could be detected in uni- and multivariate analyses that showed strain-specific levels. Differences could be assigned to specific metabolic pathways. Our results show that NMR spectroscopy is a suitable method to detect variance of the metabolome caused by subtle genetic differences. Thus, it has the potential to address genotype–phenotype associations in medaka, providing an additional level of phenotypic analysis.
format	article
author	Hannah Soergel Felix Loosli Claudia Muhle-Goll
author_facet	Hannah Soergel Felix Loosli Claudia Muhle-Goll
author_sort	Hannah Soergel
title	Strain-Specific Liver Metabolite Profiles in Medaka
title_short	Strain-Specific Liver Metabolite Profiles in Medaka
title_full	Strain-Specific Liver Metabolite Profiles in Medaka
title_fullStr	Strain-Specific Liver Metabolite Profiles in Medaka
title_full_unstemmed	Strain-Specific Liver Metabolite Profiles in Medaka
title_sort	strain-specific liver metabolite profiles in medaka
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dfe0577cb3744334b033397934db2761
work_keys_str_mv	AT hannahsoergel strainspecificlivermetaboliteprofilesinmedaka AT felixloosli strainspecificlivermetaboliteprofilesinmedaka AT claudiamuhlegoll strainspecificlivermetaboliteprofilesinmedaka
_version_	1718411351534075904

Strain-Specific Liver Metabolite Profiles in Medaka

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