Reduction of alternative electron acceptors drives biofilm formation in Shewanella algae

Abstract Shewanella spp. possess a broad respiratory versatility, which contributes to the occupation of hypoxic and anoxic environmental or host-associated niches. Here, we observe a strain-specific induction of biofilm formation in response to supplementation with the anaerobic electron acceptors...

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Autores principales:	Alberto J. Martín-Rodríguez, José A. Reyes-Darias, David Martín-Mora, José M. González, Tino Krell, Ute Römling
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/dfe0cf2af63f458ba7632cd0a262c44b
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spelling	oai:doaj.org-article:dfe0cf2af63f458ba7632cd0a262c44b2021-12-02T10:48:05ZReduction of alternative electron acceptors drives biofilm formation in Shewanella algae10.1038/s41522-020-00177-12055-5008https://doaj.org/article/dfe0cf2af63f458ba7632cd0a262c44b2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41522-020-00177-1https://doaj.org/toc/2055-5008Abstract Shewanella spp. possess a broad respiratory versatility, which contributes to the occupation of hypoxic and anoxic environmental or host-associated niches. Here, we observe a strain-specific induction of biofilm formation in response to supplementation with the anaerobic electron acceptors dimethyl sulfoxide (DMSO) and nitrate in a panel of Shewanella algae isolates. The respiration-driven biofilm response is not observed in DMSO and nitrate reductase deletion mutants of the type strain S. algae CECT 5071, and can be restored upon complementation with the corresponding reductase operon(s) but not by an operon containing a catalytically inactive nitrate reductase. The distinct transcriptional changes, proportional to the effect of these compounds on biofilm formation, include cyclic di-GMP (c-di-GMP) turnover genes. In support, ectopic expression of the c-di-GMP phosphodiesterase YhjH of Salmonella Typhimurium but not its catalytically inactive variant decreased biofilm formation. The respiration-dependent biofilm response of S. algae may permit differential colonization of environmental or host niches.Alberto J. Martín-RodríguezJosé A. Reyes-DariasDavid Martín-MoraJosé M. GonzálezTino KrellUte RömlingNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Alberto J. Martín-Rodríguez José A. Reyes-Darias David Martín-Mora José M. González Tino Krell Ute Römling Reduction of alternative electron acceptors drives biofilm formation in Shewanella algae
description	Abstract Shewanella spp. possess a broad respiratory versatility, which contributes to the occupation of hypoxic and anoxic environmental or host-associated niches. Here, we observe a strain-specific induction of biofilm formation in response to supplementation with the anaerobic electron acceptors dimethyl sulfoxide (DMSO) and nitrate in a panel of Shewanella algae isolates. The respiration-driven biofilm response is not observed in DMSO and nitrate reductase deletion mutants of the type strain S. algae CECT 5071, and can be restored upon complementation with the corresponding reductase operon(s) but not by an operon containing a catalytically inactive nitrate reductase. The distinct transcriptional changes, proportional to the effect of these compounds on biofilm formation, include cyclic di-GMP (c-di-GMP) turnover genes. In support, ectopic expression of the c-di-GMP phosphodiesterase YhjH of Salmonella Typhimurium but not its catalytically inactive variant decreased biofilm formation. The respiration-dependent biofilm response of S. algae may permit differential colonization of environmental or host niches.
format	article
author	Alberto J. Martín-Rodríguez José A. Reyes-Darias David Martín-Mora José M. González Tino Krell Ute Römling
author_facet	Alberto J. Martín-Rodríguez José A. Reyes-Darias David Martín-Mora José M. González Tino Krell Ute Römling
author_sort	Alberto J. Martín-Rodríguez
title	Reduction of alternative electron acceptors drives biofilm formation in Shewanella algae
title_short	Reduction of alternative electron acceptors drives biofilm formation in Shewanella algae
title_full	Reduction of alternative electron acceptors drives biofilm formation in Shewanella algae
title_fullStr	Reduction of alternative electron acceptors drives biofilm formation in Shewanella algae
title_full_unstemmed	Reduction of alternative electron acceptors drives biofilm formation in Shewanella algae
title_sort	reduction of alternative electron acceptors drives biofilm formation in shewanella algae
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dfe0cf2af63f458ba7632cd0a262c44b
work_keys_str_mv	AT albertojmartinrodriguez reductionofalternativeelectronacceptorsdrivesbiofilmformationinshewanellaalgae AT joseareyesdarias reductionofalternativeelectronacceptorsdrivesbiofilmformationinshewanellaalgae AT davidmartinmora reductionofalternativeelectronacceptorsdrivesbiofilmformationinshewanellaalgae AT josemgonzalez reductionofalternativeelectronacceptorsdrivesbiofilmformationinshewanellaalgae AT tinokrell reductionofalternativeelectronacceptorsdrivesbiofilmformationinshewanellaalgae AT uteromling reductionofalternativeelectronacceptorsdrivesbiofilmformationinshewanellaalgae
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Reduction of alternative electron acceptors drives biofilm formation in Shewanella algae

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