Differential Expression Patterns of TDP-43 in Single Moderate versus Repetitive Mild Traumatic Brain Injury in Mice

Traumatic brain injury (TBI) is a disabling disorder and a major cause of death and disability in the world. Both single and repetitive traumas affect the brain acutely but can also lead to chronic neurodegenerative changes. Clinical studies have shown some dissimilarities in transactive response DN...

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Autores principales:	Tamara Janković, Petra Dolenec, Jelena Rajič Bumber, Nika Gržeta, Jasna Kriz, Gordana Župan, Kristina Pilipović
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mice microglia NF-κ B postsynaptic density protein 95 traumatic brain injury transactive response DNA-binding protein 43 Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/dfebb96dfcc04465a1797aa429e0c190
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spelling	oai:doaj.org-article:dfebb96dfcc04465a1797aa429e0c1902021-11-25T17:54:22ZDifferential Expression Patterns of TDP-43 in Single Moderate versus Repetitive Mild Traumatic Brain Injury in Mice10.3390/ijms2222122111422-00671661-6596https://doaj.org/article/dfebb96dfcc04465a1797aa429e0c1902021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12211https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Traumatic brain injury (TBI) is a disabling disorder and a major cause of death and disability in the world. Both single and repetitive traumas affect the brain acutely but can also lead to chronic neurodegenerative changes. Clinical studies have shown some dissimilarities in transactive response DNA binding protein 43 (TDP-43) expression patterns following single versus repetitive TBI. We explored the acute cortical post-traumatic changes of TDP-43 using the lateral fluid percussion injury (LFPI) model of single moderate TBI in adult male mice and investigated the association of TDP-43 with post-traumatic neuroinflammation and synaptic plasticity. In the ipsilateral cortices of animals following LFPI, we found changes in the cytoplasmic and nuclear levels of TDP-43 and the decreased expression of postsynaptic protein 95 within the first 3 d post-injury. Subacute pathological changes of TDP-43 in the hippocampi of animals following LFPI and in mice exposed to repetitive mild TBI (rmTBI) were studied. Changes in the hippocampal TDP-43 expression patterns at 14 d following different brain trauma procedures showed pathological alterations only after single moderate, but not following rmTBI. Hippocampal LFPI-induced TDP-43 pathology was not accompanied by the microglial reaction, contrary to the findings after rmTBI, suggesting that different types of brain trauma may cause diverse pathophysiological changes in the brain, specifically related to the TDP-43 protein as well as to the microglial reaction. Taken together, our findings may contribute to a better understanding of the pathophysiological events following brain trauma.Tamara JankovićPetra DolenecJelena Rajič BumberNika GržetaJasna KrizGordana ŽupanKristina PilipovićMDPI AGarticlemicemicrogliaNF-κ Bpostsynaptic density protein 95traumatic brain injurytransactive response DNA-binding protein 43Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12211, p 12211 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mice microglia NF-κ B postsynaptic density protein 95 traumatic brain injury transactive response DNA-binding protein 43 Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	mice microglia NF-κ B postsynaptic density protein 95 traumatic brain injury transactive response DNA-binding protein 43 Biology (General) QH301-705.5 Chemistry QD1-999 Tamara Janković Petra Dolenec Jelena Rajič Bumber Nika Gržeta Jasna Kriz Gordana Župan Kristina Pilipović Differential Expression Patterns of TDP-43 in Single Moderate versus Repetitive Mild Traumatic Brain Injury in Mice
description	Traumatic brain injury (TBI) is a disabling disorder and a major cause of death and disability in the world. Both single and repetitive traumas affect the brain acutely but can also lead to chronic neurodegenerative changes. Clinical studies have shown some dissimilarities in transactive response DNA binding protein 43 (TDP-43) expression patterns following single versus repetitive TBI. We explored the acute cortical post-traumatic changes of TDP-43 using the lateral fluid percussion injury (LFPI) model of single moderate TBI in adult male mice and investigated the association of TDP-43 with post-traumatic neuroinflammation and synaptic plasticity. In the ipsilateral cortices of animals following LFPI, we found changes in the cytoplasmic and nuclear levels of TDP-43 and the decreased expression of postsynaptic protein 95 within the first 3 d post-injury. Subacute pathological changes of TDP-43 in the hippocampi of animals following LFPI and in mice exposed to repetitive mild TBI (rmTBI) were studied. Changes in the hippocampal TDP-43 expression patterns at 14 d following different brain trauma procedures showed pathological alterations only after single moderate, but not following rmTBI. Hippocampal LFPI-induced TDP-43 pathology was not accompanied by the microglial reaction, contrary to the findings after rmTBI, suggesting that different types of brain trauma may cause diverse pathophysiological changes in the brain, specifically related to the TDP-43 protein as well as to the microglial reaction. Taken together, our findings may contribute to a better understanding of the pathophysiological events following brain trauma.
format	article
author	Tamara Janković Petra Dolenec Jelena Rajič Bumber Nika Gržeta Jasna Kriz Gordana Župan Kristina Pilipović
author_facet	Tamara Janković Petra Dolenec Jelena Rajič Bumber Nika Gržeta Jasna Kriz Gordana Župan Kristina Pilipović
author_sort	Tamara Janković
title	Differential Expression Patterns of TDP-43 in Single Moderate versus Repetitive Mild Traumatic Brain Injury in Mice
title_short	Differential Expression Patterns of TDP-43 in Single Moderate versus Repetitive Mild Traumatic Brain Injury in Mice
title_full	Differential Expression Patterns of TDP-43 in Single Moderate versus Repetitive Mild Traumatic Brain Injury in Mice
title_fullStr	Differential Expression Patterns of TDP-43 in Single Moderate versus Repetitive Mild Traumatic Brain Injury in Mice
title_full_unstemmed	Differential Expression Patterns of TDP-43 in Single Moderate versus Repetitive Mild Traumatic Brain Injury in Mice
title_sort	differential expression patterns of tdp-43 in single moderate versus repetitive mild traumatic brain injury in mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dfebb96dfcc04465a1797aa429e0c190
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Differential Expression Patterns of TDP-43 in Single Moderate versus Repetitive Mild Traumatic Brain Injury in Mice

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