Topologically-guided continuous protein crystallization controls bacterial surface layer self-assembly

Bacteria assemble the surface layer (S-layer), a crystalline protein coat surrounding the curved surface, using protein self-assembly. Here authors image native and purified RsaA, the S-layer protein from C. crescentus, and show that protein crystallization alone is sufficient to assemble and mainta...

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Autores principales: Colin J. Comerci, Jonathan Herrmann, Joshua Yoon, Fatemeh Jabbarpour, Xiaofeng Zhou, John F. Nomellini, John Smit, Lucy Shapiro, Soichi Wakatsuki, W. E. Moerner
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/dff21d49c92d4800b6501520ddadea62
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Sumario:Bacteria assemble the surface layer (S-layer), a crystalline protein coat surrounding the curved surface, using protein self-assembly. Here authors image native and purified RsaA, the S-layer protein from C. crescentus, and show that protein crystallization alone is sufficient to assemble and maintain the S-layer in vivo.