Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection

Xuena Zhu,1 Mehenur Sarwar,1 Qiaoli Yue,2 Chunying Chen,3 Chen-Zhong Li1,4 1Nanobioengineering/Bioelectronics Laboratory, Department of Biomedical Engineering, Florida International University, Miami, FL, USA; 2Department of Chemistry, College of Chemistry and Chemical Engineering, Liao Chen Univer...

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Autores principales:	Zhu X, Sarwar M, Yue Q, Chen C, Li CZ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	8-OHdG Immunostrip Point-of-care POCTs Biosensor DNA oxidative damage Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e0009cd7011d4fd7be47640005455548
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spelling	oai:doaj.org-article:e0009cd7011d4fd7be476400054555482021-12-02T03:11:45ZBiosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection1178-2013https://doaj.org/article/e0009cd7011d4fd7be476400054555482017-02-01T00:00:00Zhttps://www.dovepress.com/biosensing-of-dna-oxidative-damage-a-model-of-using-glucose-meter-for--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xuena Zhu,1 Mehenur Sarwar,1 Qiaoli Yue,2 Chunying Chen,3 Chen-Zhong Li1,4 1Nanobioengineering/Bioelectronics Laboratory, Department of Biomedical Engineering, Florida International University, Miami, FL, USA; 2Department of Chemistry, College of Chemistry and Chemical Engineering, Liao Chen University, Shandong, 3CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, 4Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an, People’s Republic of China Abstract: Non-glucose biomarker-DNA oxidative damage biomarker 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been successfully detected using a smartphone-enabled glucose meter. Through a series of immune reactions and enzymatic reactions on a solid lateral flow platform, 8-OHdG concentration has been converted to a relative amount of glucose, and therefore can be detected by conventional glucose meter directly. The device was able to detect 8-OHdG concentrations in phosphate buffer saline as low as 1.73 ng mL-1 with a dynamic range of 1–200 ng mL-1. Considering the inherent advantages of the personal glucose meter, the demonstration of this device, therefore, should provide new opportunities for the monitoring of a wide range of biomarkers and various target analytes in connection with different molecular recognition events. Keywords: 8-OHdG, immunostrip, point-of-care, POCTs, biosensor, DNA oxidative damage Zhu XSarwar MYue QChen CLi CZDove Medical Pressarticle8-OHdGImmunostripPoint-of-carePOCTsBiosensorDNA oxidative damageMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 979-987 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	8-OHdG Immunostrip Point-of-care POCTs Biosensor DNA oxidative damage Medicine (General) R5-920
spellingShingle	8-OHdG Immunostrip Point-of-care POCTs Biosensor DNA oxidative damage Medicine (General) R5-920 Zhu X Sarwar M Yue Q Chen C Li CZ Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection
description	Xuena Zhu,1 Mehenur Sarwar,1 Qiaoli Yue,2 Chunying Chen,3 Chen-Zhong Li1,4 1Nanobioengineering/Bioelectronics Laboratory, Department of Biomedical Engineering, Florida International University, Miami, FL, USA; 2Department of Chemistry, College of Chemistry and Chemical Engineering, Liao Chen University, Shandong, 3CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, 4Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an, People’s Republic of China Abstract: Non-glucose biomarker-DNA oxidative damage biomarker 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been successfully detected using a smartphone-enabled glucose meter. Through a series of immune reactions and enzymatic reactions on a solid lateral flow platform, 8-OHdG concentration has been converted to a relative amount of glucose, and therefore can be detected by conventional glucose meter directly. The device was able to detect 8-OHdG concentrations in phosphate buffer saline as low as 1.73 ng mL-1 with a dynamic range of 1–200 ng mL-1. Considering the inherent advantages of the personal glucose meter, the demonstration of this device, therefore, should provide new opportunities for the monitoring of a wide range of biomarkers and various target analytes in connection with different molecular recognition events. Keywords: 8-OHdG, immunostrip, point-of-care, POCTs, biosensor, DNA oxidative damage
format	article
author	Zhu X Sarwar M Yue Q Chen C Li CZ
author_facet	Zhu X Sarwar M Yue Q Chen C Li CZ
author_sort	Zhu X
title	Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection
title_short	Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection
title_full	Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection
title_fullStr	Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection
title_full_unstemmed	Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection
title_sort	biosensing of dna oxidative damage: a model of using glucose meter for non-glucose biomarker detection
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/e0009cd7011d4fd7be47640005455548
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Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection

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