Physiological and proteomic analyses revealed the response mechanisms of two different drought-resistant maize varieties

Abstract Background Drought stress severely limits maize seedling growth and crop yield. Previous studies have elucidated the mechanisms by which maize acquires drought resistance and contends with water deficiency. However, the link between the physiological and molecular variations among maize cul...

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Autores principales:	Hongjie Li, Mei Yang, Chengfeng Zhao, Yifan Wang, Renhe Zhang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Drought tolerance iTRAQ Maize Photosynthesis Botany QK1-989
Acceso en línea:	https://doaj.org/article/e0089444a02149278a8338894b24a1c0
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spelling	oai:doaj.org-article:e0089444a02149278a8338894b24a1c02021-11-07T12:09:20ZPhysiological and proteomic analyses revealed the response mechanisms of two different drought-resistant maize varieties10.1186/s12870-021-03295-w1471-2229https://doaj.org/article/e0089444a02149278a8338894b24a1c02021-11-01T00:00:00Zhttps://doi.org/10.1186/s12870-021-03295-whttps://doaj.org/toc/1471-2229Abstract Background Drought stress severely limits maize seedling growth and crop yield. Previous studies have elucidated the mechanisms by which maize acquires drought resistance and contends with water deficiency. However, the link between the physiological and molecular variations among maize cultivars are unknown. Here, physiological and proteomic analyses were conducted to compare the stress responses of two maize cultivars with contrasting drought stress tolerance. Results The physiological analysis showed that the drought-tolerant SD609 maize variety maintains relatively high photochemical efficiency by enhancing its protective cyclic electron flow (CEF) mechanism and antioxidative enzymes activities. Proteomics analysis revealed that 198 and 102 proteins were differentially expressed in SD609 and the drought-sensitive SD902 cultivar, respectively. GO and KEGG enrichments indicated that SD609 upregulated proteins associated with photosynthesis, antioxidants/detoxifying enzymes, molecular chaperones and metabolic enzymes. Upregulation of the proteins related to PSII repair and photoprotection improved photochemical capacity in SD609 subjected to moderate drought stress. In SD902, however, only the molecular chaperones and sucrose synthesis pathways were induced and they failed to protect the impaired photosystem. Further analysis demonstrated that proteins related to the electron transport chain (ETC) and redox homeostasis as well as heat shock proteins (HSPs) may be important in protecting plants from drought stress. Conclusions Our experiments explored the mechanism of drought tolerance and clarified the interconnections between the physiological and proteomic factors contributing to it. In summary, our findings aid in further understanding of the drought tolerance mechanisms in maize.Hongjie LiMei YangChengfeng ZhaoYifan WangRenhe ZhangBMCarticleDrought toleranceiTRAQMaizePhotosynthesisBotanyQK1-989ENBMC Plant Biology, Vol 21, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Drought tolerance iTRAQ Maize Photosynthesis Botany QK1-989
spellingShingle	Drought tolerance iTRAQ Maize Photosynthesis Botany QK1-989 Hongjie Li Mei Yang Chengfeng Zhao Yifan Wang Renhe Zhang Physiological and proteomic analyses revealed the response mechanisms of two different drought-resistant maize varieties
description	Abstract Background Drought stress severely limits maize seedling growth and crop yield. Previous studies have elucidated the mechanisms by which maize acquires drought resistance and contends with water deficiency. However, the link between the physiological and molecular variations among maize cultivars are unknown. Here, physiological and proteomic analyses were conducted to compare the stress responses of two maize cultivars with contrasting drought stress tolerance. Results The physiological analysis showed that the drought-tolerant SD609 maize variety maintains relatively high photochemical efficiency by enhancing its protective cyclic electron flow (CEF) mechanism and antioxidative enzymes activities. Proteomics analysis revealed that 198 and 102 proteins were differentially expressed in SD609 and the drought-sensitive SD902 cultivar, respectively. GO and KEGG enrichments indicated that SD609 upregulated proteins associated with photosynthesis, antioxidants/detoxifying enzymes, molecular chaperones and metabolic enzymes. Upregulation of the proteins related to PSII repair and photoprotection improved photochemical capacity in SD609 subjected to moderate drought stress. In SD902, however, only the molecular chaperones and sucrose synthesis pathways were induced and they failed to protect the impaired photosystem. Further analysis demonstrated that proteins related to the electron transport chain (ETC) and redox homeostasis as well as heat shock proteins (HSPs) may be important in protecting plants from drought stress. Conclusions Our experiments explored the mechanism of drought tolerance and clarified the interconnections between the physiological and proteomic factors contributing to it. In summary, our findings aid in further understanding of the drought tolerance mechanisms in maize.
format	article
author	Hongjie Li Mei Yang Chengfeng Zhao Yifan Wang Renhe Zhang
author_facet	Hongjie Li Mei Yang Chengfeng Zhao Yifan Wang Renhe Zhang
author_sort	Hongjie Li
title	Physiological and proteomic analyses revealed the response mechanisms of two different drought-resistant maize varieties
title_short	Physiological and proteomic analyses revealed the response mechanisms of two different drought-resistant maize varieties
title_full	Physiological and proteomic analyses revealed the response mechanisms of two different drought-resistant maize varieties
title_fullStr	Physiological and proteomic analyses revealed the response mechanisms of two different drought-resistant maize varieties
title_full_unstemmed	Physiological and proteomic analyses revealed the response mechanisms of two different drought-resistant maize varieties
title_sort	physiological and proteomic analyses revealed the response mechanisms of two different drought-resistant maize varieties
publisher	BMC
publishDate	2021
url	https://doaj.org/article/e0089444a02149278a8338894b24a1c0
work_keys_str_mv	AT hongjieli physiologicalandproteomicanalysesrevealedtheresponsemechanismsoftwodifferentdroughtresistantmaizevarieties AT meiyang physiologicalandproteomicanalysesrevealedtheresponsemechanismsoftwodifferentdroughtresistantmaizevarieties AT chengfengzhao physiologicalandproteomicanalysesrevealedtheresponsemechanismsoftwodifferentdroughtresistantmaizevarieties AT yifanwang physiologicalandproteomicanalysesrevealedtheresponsemechanismsoftwodifferentdroughtresistantmaizevarieties AT renhezhang physiologicalandproteomicanalysesrevealedtheresponsemechanismsoftwodifferentdroughtresistantmaizevarieties
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Physiological and proteomic analyses revealed the response mechanisms of two different drought-resistant maize varieties

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