Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer Cell Lines AsPC-1 and BxPC-3

Pancreatic cancer, due to its asymptomatic development and drug-resistance, is difficult to cure. As many metallic and carbon-based nanomaterials have shown anticancer properties, we decided to investigate their potential use as anticancer agents against human pancreatic adenocarcinoma. The objectiv...

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Autores principales:	Barbara Wójcik, Ewa Sawosz, Jarosław Szczepaniak, Barbara Strojny, Malwina Sosnowska, Karolina Daniluk, Marlena Zielińska-Górska, Jaśmina Bałaban, André Chwalibog, Mateusz Wierzbicki
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	metallic nanoparticles carbon-based nanomaterials pancreatic cancer in vitro cytotoxicity Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/e00ff28972e74f568586c7a6a80749fb
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spelling	oai:doaj.org-article:e00ff28972e74f568586c7a6a80749fb2021-11-25T17:53:25ZEffects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer Cell Lines AsPC-1 and BxPC-310.3390/ijms2222121001422-00671661-6596https://doaj.org/article/e00ff28972e74f568586c7a6a80749fb2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12100https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Pancreatic cancer, due to its asymptomatic development and drug-resistance, is difficult to cure. As many metallic and carbon-based nanomaterials have shown anticancer properties, we decided to investigate their potential use as anticancer agents against human pancreatic adenocarcinoma. The objective of the study was to evaluate the toxic properties of the following nanomaterials: silver (Ag), gold (Au), platinum (Pt), graphene oxide (GO), diamond (ND), and fullerenol (C<sub>60</sub>(OH)<sub>40</sub>) against the cell lines BxPC-3, AsPC-1, HFFF-2, and HS-5. The potential cytotoxic properties were evaluated by the assessment of the cell morphology, cell viability, and cell membrane damage. The cancer cell responses to GO and ND were analysed by determination of changes in the levels of 40 different pro-inflammatory proteins. Our studies revealed that the highest cytotoxicity was obtained after the ND treatment. Moreover, BxPC-3 cells were more sensitive to ND than AsPC-1 cells due to the ND-induced ROS production. Furthermore, in both of the cancer cell lines, ND caused an increased level of IL-8 and a decreased level of TIMP-2, whereas GO caused only decreased levels of TIMP-2 and ICAM-1 proteins. This work provides important data on the toxicity of various nanoparticles against pancreatic adenocarcinoma cell lines.Barbara WójcikEwa SawoszJarosław SzczepaniakBarbara StrojnyMalwina SosnowskaKarolina DanilukMarlena Zielińska-GórskaJaśmina BałabanAndré ChwalibogMateusz WierzbickiMDPI AGarticlemetallic nanoparticlescarbon-based nanomaterialspancreatic cancerin vitrocytotoxicityBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12100, p 12100 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	metallic nanoparticles carbon-based nanomaterials pancreatic cancer in vitro cytotoxicity Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	metallic nanoparticles carbon-based nanomaterials pancreatic cancer in vitro cytotoxicity Biology (General) QH301-705.5 Chemistry QD1-999 Barbara Wójcik Ewa Sawosz Jarosław Szczepaniak Barbara Strojny Malwina Sosnowska Karolina Daniluk Marlena Zielińska-Górska Jaśmina Bałaban André Chwalibog Mateusz Wierzbicki Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer Cell Lines AsPC-1 and BxPC-3
description	Pancreatic cancer, due to its asymptomatic development and drug-resistance, is difficult to cure. As many metallic and carbon-based nanomaterials have shown anticancer properties, we decided to investigate their potential use as anticancer agents against human pancreatic adenocarcinoma. The objective of the study was to evaluate the toxic properties of the following nanomaterials: silver (Ag), gold (Au), platinum (Pt), graphene oxide (GO), diamond (ND), and fullerenol (C<sub>60</sub>(OH)<sub>40</sub>) against the cell lines BxPC-3, AsPC-1, HFFF-2, and HS-5. The potential cytotoxic properties were evaluated by the assessment of the cell morphology, cell viability, and cell membrane damage. The cancer cell responses to GO and ND were analysed by determination of changes in the levels of 40 different pro-inflammatory proteins. Our studies revealed that the highest cytotoxicity was obtained after the ND treatment. Moreover, BxPC-3 cells were more sensitive to ND than AsPC-1 cells due to the ND-induced ROS production. Furthermore, in both of the cancer cell lines, ND caused an increased level of IL-8 and a decreased level of TIMP-2, whereas GO caused only decreased levels of TIMP-2 and ICAM-1 proteins. This work provides important data on the toxicity of various nanoparticles against pancreatic adenocarcinoma cell lines.
format	article
author	Barbara Wójcik Ewa Sawosz Jarosław Szczepaniak Barbara Strojny Malwina Sosnowska Karolina Daniluk Marlena Zielińska-Górska Jaśmina Bałaban André Chwalibog Mateusz Wierzbicki
author_facet	Barbara Wójcik Ewa Sawosz Jarosław Szczepaniak Barbara Strojny Malwina Sosnowska Karolina Daniluk Marlena Zielińska-Górska Jaśmina Bałaban André Chwalibog Mateusz Wierzbicki
author_sort	Barbara Wójcik
title	Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer Cell Lines AsPC-1 and BxPC-3
title_short	Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer Cell Lines AsPC-1 and BxPC-3
title_full	Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer Cell Lines AsPC-1 and BxPC-3
title_fullStr	Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer Cell Lines AsPC-1 and BxPC-3
title_full_unstemmed	Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer Cell Lines AsPC-1 and BxPC-3
title_sort	effects of metallic and carbon-based nanomaterials on human pancreatic cancer cell lines aspc-1 and bxpc-3
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e00ff28972e74f568586c7a6a80749fb
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Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer Cell Lines AsPC-1 and BxPC-3

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