Polymersomes containing quantum dots for cellular imaging

Marine Camblin,1 Pascal Detampel,1 Helene Kettiger,1 Dalin Wu,2 Vimalkumar Balasubramanian,1,* Jörg Huwyler1,*1Division of Pharmaceutical Technology, 2Department of Chemistry, University of Basel, Basel, Switzerland*These authors contributed equally to this workAbstract: Quantum dots (QDs)...

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Autores principales:	Camblin M, Detampel P, Kettiger H, Wu D, Balasubramanian V, Huwyler J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e010aa94a64345a1a5ad2ddf370a1ed8
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spelling	oai:doaj.org-article:e010aa94a64345a1a5ad2ddf370a1ed82021-12-02T08:07:45ZPolymersomes containing quantum dots for cellular imaging1178-2013https://doaj.org/article/e010aa94a64345a1a5ad2ddf370a1ed82014-05-01T00:00:00Zhttp://www.dovepress.com/polymersomes-containing-quantum-dots-for-cellular-imaging-a16794https://doaj.org/toc/1178-2013 Marine Camblin,1 Pascal Detampel,1 Helene Kettiger,1 Dalin Wu,2 Vimalkumar Balasubramanian,1,* Jörg Huwyler1,1Division of Pharmaceutical Technology, 2Department of Chemistry, University of Basel, Basel, SwitzerlandThese authors contributed equally to this workAbstract: Quantum dots (QDs) are highly fluorescent and stable probes for cellular and molecular imaging. However, poor intracellular delivery, stability, and toxicity of QDs in biological compartments hamper their use in cellular imaging. To overcome these limitations, we developed a simple and effective method to load QDs into polymersomes (Ps) made of poly(dimethylsiloxane)-poly(2-methyloxazoline) (PDMS-PMOXA) diblock copolymers without compromising the characteristics of the QDs. These Ps showed no cellular toxicity and QDs were successfully incorporated into the aqueous compartment of the Ps as confirmed by transmission electron microscopy, fluorescence spectroscopy, and fluorescence correlation spectroscopy. Ps containing QDs showed colloidal stability over a period of 6 weeks if stored in phosphate-buffered saline (PBS) at physiological pH (7.4). Efficient intracellular delivery of Ps containing QDs was achieved in human liver carcinoma cells (HepG2) and was visualized by confocal laser scanning microscopy (CLSM). Ps containing QDs showed a time- and concentration-dependent uptake in HepG2 cells and exhibited better intracellular stability than liposomes. Our results suggest that Ps containing QDs can be used as nanoprobes for cellular imaging.Keywords: quantum dots, polymersomes, cellular imaging, cellular uptakeCamblin MDetampel PKettiger HWu DBalasubramanian VHuwyler JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2287-2298 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Camblin M Detampel P Kettiger H Wu D Balasubramanian V Huwyler J Polymersomes containing quantum dots for cellular imaging
description	Marine Camblin,1 Pascal Detampel,1 Helene Kettiger,1 Dalin Wu,2 Vimalkumar Balasubramanian,1,* Jörg Huwyler1,1Division of Pharmaceutical Technology, 2Department of Chemistry, University of Basel, Basel, SwitzerlandThese authors contributed equally to this workAbstract: Quantum dots (QDs) are highly fluorescent and stable probes for cellular and molecular imaging. However, poor intracellular delivery, stability, and toxicity of QDs in biological compartments hamper their use in cellular imaging. To overcome these limitations, we developed a simple and effective method to load QDs into polymersomes (Ps) made of poly(dimethylsiloxane)-poly(2-methyloxazoline) (PDMS-PMOXA) diblock copolymers without compromising the characteristics of the QDs. These Ps showed no cellular toxicity and QDs were successfully incorporated into the aqueous compartment of the Ps as confirmed by transmission electron microscopy, fluorescence spectroscopy, and fluorescence correlation spectroscopy. Ps containing QDs showed colloidal stability over a period of 6 weeks if stored in phosphate-buffered saline (PBS) at physiological pH (7.4). Efficient intracellular delivery of Ps containing QDs was achieved in human liver carcinoma cells (HepG2) and was visualized by confocal laser scanning microscopy (CLSM). Ps containing QDs showed a time- and concentration-dependent uptake in HepG2 cells and exhibited better intracellular stability than liposomes. Our results suggest that Ps containing QDs can be used as nanoprobes for cellular imaging.Keywords: quantum dots, polymersomes, cellular imaging, cellular uptake
format	article
author	Camblin M Detampel P Kettiger H Wu D Balasubramanian V Huwyler J
author_facet	Camblin M Detampel P Kettiger H Wu D Balasubramanian V Huwyler J
author_sort	Camblin M
title	Polymersomes containing quantum dots for cellular imaging
title_short	Polymersomes containing quantum dots for cellular imaging
title_full	Polymersomes containing quantum dots for cellular imaging
title_fullStr	Polymersomes containing quantum dots for cellular imaging
title_full_unstemmed	Polymersomes containing quantum dots for cellular imaging
title_sort	polymersomes containing quantum dots for cellular imaging
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/e010aa94a64345a1a5ad2ddf370a1ed8
work_keys_str_mv	AT camblinm polymersomescontainingquantumdotsforcellularimaging AT detampelp polymersomescontainingquantumdotsforcellularimaging AT kettigerh polymersomescontainingquantumdotsforcellularimaging AT wud polymersomescontainingquantumdotsforcellularimaging AT balasubramanianv polymersomescontainingquantumdotsforcellularimaging AT huwylerj polymersomescontainingquantumdotsforcellularimaging
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Polymersomes containing quantum dots for cellular imaging

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