A rapid FACS-based strategy to isolate human gene knockin and knockout clones.

Gene targeting protocols for mammalian cells remain inefficient and labor intensive. Here we describe FASTarget, a rapid, fluorescent cell sorting based strategy to isolate rare gene targeting events in human somatic cells. A fluorescent protein is used as a means for direct selection of targeted cl...

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Autores principales: João F Mata, Telma Lopes, Rui Gardner, Lars E T Jansen
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2012
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Acceso en línea:https://doaj.org/article/e011229d1c35449bad834876954edec2
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