A rapid FACS-based strategy to isolate human gene knockin and knockout clones.
Gene targeting protocols for mammalian cells remain inefficient and labor intensive. Here we describe FASTarget, a rapid, fluorescent cell sorting based strategy to isolate rare gene targeting events in human somatic cells. A fluorescent protein is used as a means for direct selection of targeted cl...
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Autores principales: | João F Mata, Telma Lopes, Rui Gardner, Lars E T Jansen |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2012
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Materias: | |
Acceso en línea: | https://doaj.org/article/e011229d1c35449bad834876954edec2 |
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