Preparation of <i>Komagataeibacter xylinus</i> Inoculum for Bacterial Cellulose Biosynthesis Using Magnetically Assisted External-Loop Airlift Bioreactor

Preparation of <i>Komagataeibacter xylinus</i> Inoculum for Bacterial Cellulose Biosynthesis Using Magnetically Assisted External-Loop Airlift Bioreactor

The aim of this study was to demonstrate the applicability of a novel magnetically assisted external-loop airlift bioreactor (EL-ALB), equipped with rotating magnetic field (RMF) generators for the preparation of <i>Komagataeibacter</i><i>xylinus</i> inoculum during three-cyc...

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Autores principales: Anna Żywicka, Daria Ciecholewska-Juśko, Radosław Drozd, Rafał Rakoczy, Maciej Konopacki, Marian Kordas, Adam Junka, Paweł Migdał, Karol Fijałkowski
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
rotating magnetic field
airlift bioreactor
inoculum
bacterial cellulose
fermentation
Organic chemistry
QD241-441
Acceso en línea:https://doaj.org/article/e011b0035b16479ea5d7283ba5934e57
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spelling oai:doaj.org-article:e011b0035b16479ea5d7283ba5934e572021-11-25T18:48:42ZPreparation of <i>Komagataeibacter xylinus</i> Inoculum for Bacterial Cellulose Biosynthesis Using Magnetically Assisted External-Loop Airlift Bioreactor10.3390/polym132239502073-4360https://doaj.org/article/e011b0035b16479ea5d7283ba5934e572021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/22/3950https://doaj.org/toc/2073-4360The aim of this study was to demonstrate the applicability of a novel magnetically assisted external-loop airlift bioreactor (EL-ALB), equipped with rotating magnetic field (RMF) generators for the preparation of <i>Komagataeibacter</i><i>xylinus</i> inoculum during three-cycle repeated fed-batch cultures, further used for bacterial cellulose (BC) production. The fermentation carried out in the RMF-assisted EL-ALB allowed to obtain an inoculum of more than 200× higher cellular density compared to classical methods of inoculum preparation. The inoculum obtained in the RMF-assisted EL-ALB was characterized by a high and stable metabolic activity during repeated batch fermentation process. The application of the RMF-assisted EL-ALB for <i>K. xylinus</i> inoculum production did not induce the formation of cellulose-deficient mutants. It was also confirmed that the ability of <i>K. xylinus</i> to produce BC was at the same level (7.26 g/L of dry mass), regardless of inoculum age. Additionally, the BC obtained from the inoculum produced in the RMF-assisted EL-ALB was characterized by reproducible water-related properties, mechanical strength, nano-fibrillar structure and total crystallinity index. The lack of any negative impact of inoculum preparation method using RMF-assisted EL-ALB on BC properties is of paramount value for its future applications, including use as a biomaterial in tissue engineering, wound healing, and drug delivery, where especially BC liquid capacity, nanostructure, crystallinity, and mechanical properties play essential roles.Anna ŻywickaDaria Ciecholewska-JuśkoRadosław DrozdRafał RakoczyMaciej KonopackiMarian KordasAdam JunkaPaweł MigdałKarol FijałkowskiMDPI AGarticlerotating magnetic fieldairlift bioreactorinoculumbacterial cellulosefermentationOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3950, p 3950 (2021)
institution DOAJ
collection DOAJ
language EN
topic rotating magnetic field
airlift bioreactor
inoculum
bacterial cellulose
fermentation
Organic chemistry
QD241-441
spellingShingle rotating magnetic field
airlift bioreactor
inoculum
bacterial cellulose
fermentation
Organic chemistry
QD241-441
Anna Żywicka
Daria Ciecholewska-Juśko
Radosław Drozd
Rafał Rakoczy
Maciej Konopacki
Marian Kordas
Adam Junka
Paweł Migdał
Karol Fijałkowski
Preparation of <i>Komagataeibacter xylinus</i> Inoculum for Bacterial Cellulose Biosynthesis Using Magnetically Assisted External-Loop Airlift Bioreactor
description The aim of this study was to demonstrate the applicability of a novel magnetically assisted external-loop airlift bioreactor (EL-ALB), equipped with rotating magnetic field (RMF) generators for the preparation of <i>Komagataeibacter</i><i>xylinus</i> inoculum during three-cycle repeated fed-batch cultures, further used for bacterial cellulose (BC) production. The fermentation carried out in the RMF-assisted EL-ALB allowed to obtain an inoculum of more than 200× higher cellular density compared to classical methods of inoculum preparation. The inoculum obtained in the RMF-assisted EL-ALB was characterized by a high and stable metabolic activity during repeated batch fermentation process. The application of the RMF-assisted EL-ALB for <i>K. xylinus</i> inoculum production did not induce the formation of cellulose-deficient mutants. It was also confirmed that the ability of <i>K. xylinus</i> to produce BC was at the same level (7.26 g/L of dry mass), regardless of inoculum age. Additionally, the BC obtained from the inoculum produced in the RMF-assisted EL-ALB was characterized by reproducible water-related properties, mechanical strength, nano-fibrillar structure and total crystallinity index. The lack of any negative impact of inoculum preparation method using RMF-assisted EL-ALB on BC properties is of paramount value for its future applications, including use as a biomaterial in tissue engineering, wound healing, and drug delivery, where especially BC liquid capacity, nanostructure, crystallinity, and mechanical properties play essential roles.
format article
author Anna Żywicka
Daria Ciecholewska-Juśko
Radosław Drozd
Rafał Rakoczy
Maciej Konopacki
Marian Kordas
Adam Junka
Paweł Migdał
Karol Fijałkowski
author_facet Anna Żywicka
Daria Ciecholewska-Juśko
Radosław Drozd
Rafał Rakoczy
Maciej Konopacki
Marian Kordas
Adam Junka
Paweł Migdał
Karol Fijałkowski
author_sort Anna Żywicka
title Preparation of <i>Komagataeibacter xylinus</i> Inoculum for Bacterial Cellulose Biosynthesis Using Magnetically Assisted External-Loop Airlift Bioreactor
title_short Preparation of <i>Komagataeibacter xylinus</i> Inoculum for Bacterial Cellulose Biosynthesis Using Magnetically Assisted External-Loop Airlift Bioreactor
title_full Preparation of <i>Komagataeibacter xylinus</i> Inoculum for Bacterial Cellulose Biosynthesis Using Magnetically Assisted External-Loop Airlift Bioreactor
title_fullStr Preparation of <i>Komagataeibacter xylinus</i> Inoculum for Bacterial Cellulose Biosynthesis Using Magnetically Assisted External-Loop Airlift Bioreactor
title_full_unstemmed Preparation of <i>Komagataeibacter xylinus</i> Inoculum for Bacterial Cellulose Biosynthesis Using Magnetically Assisted External-Loop Airlift Bioreactor
title_sort preparation of <i>komagataeibacter xylinus</i> inoculum for bacterial cellulose biosynthesis using magnetically assisted external-loop airlift bioreactor
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/e011b0035b16479ea5d7283ba5934e57
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