Survival of animal and human-associated Listeria monocytogenes in drinking water and biofilms

Land slugs are occasionally observed as contaminants in groundwater wells and drinking water treatment plants including storage tanks. Slugs may feed on carrion and feces, and they are potential vectors of pathogens such as Listeria monocytogenes. We isolated L. monocytogenes from the pest slug Ario...

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Autores principales:	Louise Appel Bjergbæk, Martin Hesselsoe, Sussie Pagh, Peter Roslev
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	drinking water and biofilms escherichia coli listeria monocytogenes slugs survival Water supply for domestic and industrial purposes TD201-500 River, lake, and water-supply engineering (General) TC401-506
Acceso en línea:	https://doaj.org/article/e0160bf01a0d46a7a1c5ca4f6bd09c28
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spelling	oai:doaj.org-article:e0160bf01a0d46a7a1c5ca4f6bd09c282021-11-23T18:56:00ZSurvival of animal and human-associated Listeria monocytogenes in drinking water and biofilms1606-97491607-079810.2166/ws.2021.116https://doaj.org/article/e0160bf01a0d46a7a1c5ca4f6bd09c282021-11-01T00:00:00Zhttp://ws.iwaponline.com/content/21/7/3515https://doaj.org/toc/1606-9749https://doaj.org/toc/1607-0798Land slugs are occasionally observed as contaminants in groundwater wells and drinking water treatment plants including storage tanks. Slugs may feed on carrion and feces, and they are potential vectors of pathogens such as Listeria monocytogenes. We isolated L. monocytogenes from the pest slug Arion vulgaris and examined the persistence and survival of human- and slug-derived L. monocytogenes in groundwater-based drinking water and biofilms. L. monocytogenes survival was evaluated using cultivation and cultivation-independent techniques. L. monocytogenes remained culturable for 35–47 days in drinking water with first-order decay rates between 0.314 and 0.457 h−1 (T99 ≥10 days). Attachment of L. monocytogenes to filter sand delayed washout from drinking water filters and increased persistence 2–3-fold. Indigenous biofilms stimulated initial surface attachment 10–100-fold but L. monocytogenes declined more rapidly in drinking water biofilms compared with virgin filters not colonized by microorganisms. Grazing by protozoa likely attenuated L. monocytogenes survival in some drinking water biofilms. A comparable survival pattern was observed for L. monocytogenes and the fecal indicator bacterium E. coli. The study suggests that live L. monocytogenes can persist for weeks as sessile organisms in groundwater-based drinking water supplies and may subsequently be released to the drinking water. HIGHLIGHTS This is likely the first study examining the persistence of Listeria monocytogenes from humans and slugs in drinking water and biofilms.; Land slugs can contaminante groundwater wells, drinking water treatment plants and water storage tanks. Slugs feed on carrion and feces, and are potential vectors of L. monocytogenes.; Live L. monocytogenes can survive for weeks in drinking water and filter material may increase persistence.;Louise Appel BjergbækMartin HesselsoeSussie PaghPeter RoslevIWA Publishingarticledrinking water and biofilmsescherichia colilisteria monocytogenesslugssurvivalWater supply for domestic and industrial purposesTD201-500River, lake, and water-supply engineering (General)TC401-506ENWater Supply, Vol 21, Iss 7, Pp 3515-3525 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	drinking water and biofilms escherichia coli listeria monocytogenes slugs survival Water supply for domestic and industrial purposes TD201-500 River, lake, and water-supply engineering (General) TC401-506
spellingShingle	drinking water and biofilms escherichia coli listeria monocytogenes slugs survival Water supply for domestic and industrial purposes TD201-500 River, lake, and water-supply engineering (General) TC401-506 Louise Appel Bjergbæk Martin Hesselsoe Sussie Pagh Peter Roslev Survival of animal and human-associated Listeria monocytogenes in drinking water and biofilms
description	Land slugs are occasionally observed as contaminants in groundwater wells and drinking water treatment plants including storage tanks. Slugs may feed on carrion and feces, and they are potential vectors of pathogens such as Listeria monocytogenes. We isolated L. monocytogenes from the pest slug Arion vulgaris and examined the persistence and survival of human- and slug-derived L. monocytogenes in groundwater-based drinking water and biofilms. L. monocytogenes survival was evaluated using cultivation and cultivation-independent techniques. L. monocytogenes remained culturable for 35–47 days in drinking water with first-order decay rates between 0.314 and 0.457 h−1 (T99 ≥10 days). Attachment of L. monocytogenes to filter sand delayed washout from drinking water filters and increased persistence 2–3-fold. Indigenous biofilms stimulated initial surface attachment 10–100-fold but L. monocytogenes declined more rapidly in drinking water biofilms compared with virgin filters not colonized by microorganisms. Grazing by protozoa likely attenuated L. monocytogenes survival in some drinking water biofilms. A comparable survival pattern was observed for L. monocytogenes and the fecal indicator bacterium E. coli. The study suggests that live L. monocytogenes can persist for weeks as sessile organisms in groundwater-based drinking water supplies and may subsequently be released to the drinking water. HIGHLIGHTS This is likely the first study examining the persistence of Listeria monocytogenes from humans and slugs in drinking water and biofilms.; Land slugs can contaminante groundwater wells, drinking water treatment plants and water storage tanks. Slugs feed on carrion and feces, and are potential vectors of L. monocytogenes.; Live L. monocytogenes can survive for weeks in drinking water and filter material may increase persistence.;
format	article
author	Louise Appel Bjergbæk Martin Hesselsoe Sussie Pagh Peter Roslev
author_facet	Louise Appel Bjergbæk Martin Hesselsoe Sussie Pagh Peter Roslev
author_sort	Louise Appel Bjergbæk
title	Survival of animal and human-associated Listeria monocytogenes in drinking water and biofilms
title_short	Survival of animal and human-associated Listeria monocytogenes in drinking water and biofilms
title_full	Survival of animal and human-associated Listeria monocytogenes in drinking water and biofilms
title_fullStr	Survival of animal and human-associated Listeria monocytogenes in drinking water and biofilms
title_full_unstemmed	Survival of animal and human-associated Listeria monocytogenes in drinking water and biofilms
title_sort	survival of animal and human-associated listeria monocytogenes in drinking water and biofilms
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/e0160bf01a0d46a7a1c5ca4f6bd09c28
work_keys_str_mv	AT louiseappelbjergbæk survivalofanimalandhumanassociatedlisteriamonocytogenesindrinkingwaterandbiofilms AT martinhesselsoe survivalofanimalandhumanassociatedlisteriamonocytogenesindrinkingwaterandbiofilms AT sussiepagh survivalofanimalandhumanassociatedlisteriamonocytogenesindrinkingwaterandbiofilms AT peterroslev survivalofanimalandhumanassociatedlisteriamonocytogenesindrinkingwaterandbiofilms
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Survival of animal and human-associated Listeria monocytogenes in drinking water and biofilms

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