In vitro evaluation of folic acid-conjugated redox-responsive mesoporous silica nanoparticles for the delivery of cisplatin

Merlis P Alvarez-Berríos,1 Juan L Vivero-Escoto2,3 1Department of Science and Technology, Inter American University of Puerto Rico, Ponce, Puerto Rico, 2Department of Chemistry, 3Center for Biomedical Engineering and Science, University of North Carolina at Charlotte, Charlotte, NC, USA...

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Autores principales:	Alvarez-Berríos MP, Vivero-Escoto JL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Cancer treatment cisplatin prodrug intracellular delivery folic acid mesoporous silica. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e019296394f946839ef640a63eb6f14c
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spelling	oai:doaj.org-article:e019296394f946839ef640a63eb6f14c2021-12-02T03:05:14ZIn vitro evaluation of folic acid-conjugated redox-responsive mesoporous silica nanoparticles for the delivery of cisplatin1178-2013https://doaj.org/article/e019296394f946839ef640a63eb6f14c2016-11-01T00:00:00Zhttps://www.dovepress.com/in-vitro-evaluation-of-folic-acid-conjugated-redox-responsive-mesoporo-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Merlis P Alvarez-Berríos,1 Juan L Vivero-Escoto2,3 1Department of Science and Technology, Inter American University of Puerto Rico, Ponce, Puerto Rico, 2Department of Chemistry, 3Center for Biomedical Engineering and Science, University of North Carolina at Charlotte, Charlotte, NC, USA Abstract: The use of cisplatin(IV) prodrugs for the delivery of cisplatin have gained significant attention, because of their low toxicity and reactivity. Recent studies have shown that targeted cisplatin(IV)-prodrug nanoparticle-based delivery systems can improve the internalization of the cisplatin(IV) prodrug. We hypothesized that folic acid-conjugated mesoporous silica nanoparticles (MSNs) containing cisplatin(IV) prodrug could target cancer cells that overexpress the folate receptor and deliver the active cisplatin drug upon intracellular reduction. To prove this hypothesis, internalization and localization studies in HeLa cancer cells were performed using flow cytometry and confocal microscopy. The ability of MSNs to escape from the endolysosomal compartments, the formation of DNA adducts, and the cytotoxic effects of the MSNs were also evaluated. Our results confirmed that this MSN-based delivery platform was capable of delivering cisplatin into the cytosol of HeLa cells, inducing DNA adducts and subsequent cell death. Keywords: cancer treatment, cisplatin prodrug, intracellular delivery, folic acid, mesoporous silicaAlvarez-Berríos MPVivero-Escoto JLDove Medical PressarticleCancer treatmentcisplatin prodrugintracellular deliveryfolic acidmesoporous silica.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 6251-6265 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cancer treatment cisplatin prodrug intracellular delivery folic acid mesoporous silica. Medicine (General) R5-920
spellingShingle	Cancer treatment cisplatin prodrug intracellular delivery folic acid mesoporous silica. Medicine (General) R5-920 Alvarez-Berríos MP Vivero-Escoto JL In vitro evaluation of folic acid-conjugated redox-responsive mesoporous silica nanoparticles for the delivery of cisplatin
description	Merlis P Alvarez-Berríos,1 Juan L Vivero-Escoto2,3 1Department of Science and Technology, Inter American University of Puerto Rico, Ponce, Puerto Rico, 2Department of Chemistry, 3Center for Biomedical Engineering and Science, University of North Carolina at Charlotte, Charlotte, NC, USA Abstract: The use of cisplatin(IV) prodrugs for the delivery of cisplatin have gained significant attention, because of their low toxicity and reactivity. Recent studies have shown that targeted cisplatin(IV)-prodrug nanoparticle-based delivery systems can improve the internalization of the cisplatin(IV) prodrug. We hypothesized that folic acid-conjugated mesoporous silica nanoparticles (MSNs) containing cisplatin(IV) prodrug could target cancer cells that overexpress the folate receptor and deliver the active cisplatin drug upon intracellular reduction. To prove this hypothesis, internalization and localization studies in HeLa cancer cells were performed using flow cytometry and confocal microscopy. The ability of MSNs to escape from the endolysosomal compartments, the formation of DNA adducts, and the cytotoxic effects of the MSNs were also evaluated. Our results confirmed that this MSN-based delivery platform was capable of delivering cisplatin into the cytosol of HeLa cells, inducing DNA adducts and subsequent cell death. Keywords: cancer treatment, cisplatin prodrug, intracellular delivery, folic acid, mesoporous silica
format	article
author	Alvarez-Berríos MP Vivero-Escoto JL
author_facet	Alvarez-Berríos MP Vivero-Escoto JL
author_sort	Alvarez-Berríos MP
title	In vitro evaluation of folic acid-conjugated redox-responsive mesoporous silica nanoparticles for the delivery of cisplatin
title_short	In vitro evaluation of folic acid-conjugated redox-responsive mesoporous silica nanoparticles for the delivery of cisplatin
title_full	In vitro evaluation of folic acid-conjugated redox-responsive mesoporous silica nanoparticles for the delivery of cisplatin
title_fullStr	In vitro evaluation of folic acid-conjugated redox-responsive mesoporous silica nanoparticles for the delivery of cisplatin
title_full_unstemmed	In vitro evaluation of folic acid-conjugated redox-responsive mesoporous silica nanoparticles for the delivery of cisplatin
title_sort	in vitro evaluation of folic acid-conjugated redox-responsive mesoporous silica nanoparticles for the delivery of cisplatin
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/e019296394f946839ef640a63eb6f14c
work_keys_str_mv	AT alvarezberriosmp invitroevaluationoffolicacidconjugatedredoxresponsivemesoporoussilicananoparticlesforthedeliveryofcisplatin AT viveroescotojl invitroevaluationoffolicacidconjugatedredoxresponsivemesoporoussilicananoparticlesforthedeliveryofcisplatin
_version_	1718402012759982080

In vitro evaluation of folic acid-conjugated redox-responsive mesoporous silica nanoparticles for the delivery of cisplatin

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