Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives

Abstract Wood extractives, solvent-soluble fractions of woody biomass, are considered to be a factor impeding or excluding fungal colonization on the freshly harvested conifers. Among wood decay fungi, the basidiomycete Phlebiopsis gigantea has evolved a unique enzyme system to efficiently transform...

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Autores principales:	Mana Iwata, Ana Gutiérrez, Gisela Marques, Grzegorz Sabat, Philip J. Kersten, Daniel Cullen, Jennifer M. Bhatnagar, Jagjit Yadav, Anna Lipzen, Yuko Yoshinaga, Aditi Sharma, Catherine Adam, Christopher Daum, Vivian Ng, Igor V. Grigoriev, Chiaki Hori
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e01ccf914d544e53acf51a7c0278c73f
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spelling	oai:doaj.org-article:e01ccf914d544e53acf51a7c0278c73f2021-12-02T17:41:27ZOmics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives10.1038/s41598-021-91756-52045-2322https://doaj.org/article/e01ccf914d544e53acf51a7c0278c73f2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91756-5https://doaj.org/toc/2045-2322Abstract Wood extractives, solvent-soluble fractions of woody biomass, are considered to be a factor impeding or excluding fungal colonization on the freshly harvested conifers. Among wood decay fungi, the basidiomycete Phlebiopsis gigantea has evolved a unique enzyme system to efficiently transform or degrade conifer extractives but little is known about the mechanism(s). In this study, to clarify the mechanism(s) of softwood degradation, we examined the transcriptome, proteome, and metabolome of P. gigantea when grown on defined media containing microcrystalline cellulose and pine sapwood extractives. Beyond the conventional enzymes often associated with cellulose, hemicellulose and lignin degradation, an array of enzymes implicated in the metabolism of softwood lipophilic extractives such as fatty and resin acids, steroids and glycerides was significantly up-regulated. Among these, a highly expressed and inducible lipase is likely responsible for lipophilic extractive degradation, based on its extracellular location and our characterization of the recombinant enzyme. Our results provide insight into physiological roles of extractives in the interaction between wood and fungi.Mana IwataAna GutiérrezGisela MarquesGrzegorz SabatPhilip J. KerstenDaniel CullenJennifer M. BhatnagarJagjit YadavAnna LipzenYuko YoshinagaAditi SharmaCatherine AdamChristopher DaumVivian NgIgor V. GrigorievChiaki HoriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mana Iwata Ana Gutiérrez Gisela Marques Grzegorz Sabat Philip J. Kersten Daniel Cullen Jennifer M. Bhatnagar Jagjit Yadav Anna Lipzen Yuko Yoshinaga Aditi Sharma Catherine Adam Christopher Daum Vivian Ng Igor V. Grigoriev Chiaki Hori Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives
description	Abstract Wood extractives, solvent-soluble fractions of woody biomass, are considered to be a factor impeding or excluding fungal colonization on the freshly harvested conifers. Among wood decay fungi, the basidiomycete Phlebiopsis gigantea has evolved a unique enzyme system to efficiently transform or degrade conifer extractives but little is known about the mechanism(s). In this study, to clarify the mechanism(s) of softwood degradation, we examined the transcriptome, proteome, and metabolome of P. gigantea when grown on defined media containing microcrystalline cellulose and pine sapwood extractives. Beyond the conventional enzymes often associated with cellulose, hemicellulose and lignin degradation, an array of enzymes implicated in the metabolism of softwood lipophilic extractives such as fatty and resin acids, steroids and glycerides was significantly up-regulated. Among these, a highly expressed and inducible lipase is likely responsible for lipophilic extractive degradation, based on its extracellular location and our characterization of the recombinant enzyme. Our results provide insight into physiological roles of extractives in the interaction between wood and fungi.
format	article
author	Mana Iwata Ana Gutiérrez Gisela Marques Grzegorz Sabat Philip J. Kersten Daniel Cullen Jennifer M. Bhatnagar Jagjit Yadav Anna Lipzen Yuko Yoshinaga Aditi Sharma Catherine Adam Christopher Daum Vivian Ng Igor V. Grigoriev Chiaki Hori
author_facet	Mana Iwata Ana Gutiérrez Gisela Marques Grzegorz Sabat Philip J. Kersten Daniel Cullen Jennifer M. Bhatnagar Jagjit Yadav Anna Lipzen Yuko Yoshinaga Aditi Sharma Catherine Adam Christopher Daum Vivian Ng Igor V. Grigoriev Chiaki Hori
author_sort	Mana Iwata
title	Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives
title_short	Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives
title_full	Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives
title_fullStr	Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives
title_full_unstemmed	Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives
title_sort	omics analyses and biochemical study of phlebiopsis gigantea elucidate its degradation strategy of wood extractives
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e01ccf914d544e53acf51a7c0278c73f
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Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives

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