A man-made ATP-binding protein evolved independent of nature causes abnormal growth in bacterial cells.

Recent advances in de novo protein evolution have made it possible to create synthetic proteins from unbiased libraries that fold into stable tertiary structures with predefined functions. However, it is not known whether such proteins will be functional when expressed inside living cells or how a h...

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Autores principales:	Joshua M Stomel, James W Wilson, Megan A León, Phillip Stafford, John C Chaput
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e0215494bed24e8781ac1772fa2da4c3
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id	oai:doaj.org-article:e0215494bed24e8781ac1772fa2da4c3
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spelling	oai:doaj.org-article:e0215494bed24e8781ac1772fa2da4c32021-11-25T06:19:50ZA man-made ATP-binding protein evolved independent of nature causes abnormal growth in bacterial cells.1932-620310.1371/journal.pone.0007385https://doaj.org/article/e0215494bed24e8781ac1772fa2da4c32009-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19812699/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Recent advances in de novo protein evolution have made it possible to create synthetic proteins from unbiased libraries that fold into stable tertiary structures with predefined functions. However, it is not known whether such proteins will be functional when expressed inside living cells or how a host organism would respond to an encounter with a non-biological protein. Here, we examine the physiology and morphology of Escherichia coli cells engineered to express a synthetic ATP-binding protein evolved entirely from non-biological origins. We show that this man-made protein disrupts the normal energetic balance of the cell by altering the levels of intracellular ATP. This disruption cascades into a series of events that ultimately limit reproductive competency by inhibiting cell division. We now describe a detailed investigation into the synthetic biology of this man-made protein in a living bacterial organism, and the effect that this protein has on normal cell physiology.Joshua M StomelJames W WilsonMegan A LeónPhillip StaffordJohn C ChaputPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 10, p e7385 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joshua M Stomel James W Wilson Megan A León Phillip Stafford John C Chaput A man-made ATP-binding protein evolved independent of nature causes abnormal growth in bacterial cells.
description	Recent advances in de novo protein evolution have made it possible to create synthetic proteins from unbiased libraries that fold into stable tertiary structures with predefined functions. However, it is not known whether such proteins will be functional when expressed inside living cells or how a host organism would respond to an encounter with a non-biological protein. Here, we examine the physiology and morphology of Escherichia coli cells engineered to express a synthetic ATP-binding protein evolved entirely from non-biological origins. We show that this man-made protein disrupts the normal energetic balance of the cell by altering the levels of intracellular ATP. This disruption cascades into a series of events that ultimately limit reproductive competency by inhibiting cell division. We now describe a detailed investigation into the synthetic biology of this man-made protein in a living bacterial organism, and the effect that this protein has on normal cell physiology.
format	article
author	Joshua M Stomel James W Wilson Megan A León Phillip Stafford John C Chaput
author_facet	Joshua M Stomel James W Wilson Megan A León Phillip Stafford John C Chaput
author_sort	Joshua M Stomel
title	A man-made ATP-binding protein evolved independent of nature causes abnormal growth in bacterial cells.
title_short	A man-made ATP-binding protein evolved independent of nature causes abnormal growth in bacterial cells.
title_full	A man-made ATP-binding protein evolved independent of nature causes abnormal growth in bacterial cells.
title_fullStr	A man-made ATP-binding protein evolved independent of nature causes abnormal growth in bacterial cells.
title_full_unstemmed	A man-made ATP-binding protein evolved independent of nature causes abnormal growth in bacterial cells.
title_sort	man-made atp-binding protein evolved independent of nature causes abnormal growth in bacterial cells.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/e0215494bed24e8781ac1772fa2da4c3
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A man-made ATP-binding protein evolved independent of nature causes abnormal growth in bacterial cells.

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