Plasma amino acid concentrations during experimental hyperinsulinemia in 2 laminitis models

Abstract Background Endocrinopathic laminitis develops in association with insulin dysregulation, but the role of insulin in the pathogenesis remains unclear. Hyperinsulinemia can cause hypoaminoacidemia, which is associated with integumentary lesions in other species and therefore warrants investig...

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Autores principales:	Simon M. Stokes, Darko Stefanovski, François‐René Bertin, Carlos E. Medina‐Torres, James K. Belknap, Andrew W. vanEps
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	amino acids endocrinopathic horse hypoaminoacidemia insulin Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/e02688ff27ee459e8a6ce672a2f7d64a
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spelling	oai:doaj.org-article:e02688ff27ee459e8a6ce672a2f7d64a2021-11-30T17:01:05ZPlasma amino acid concentrations during experimental hyperinsulinemia in 2 laminitis models1939-16760891-664010.1111/jvim.16095https://doaj.org/article/e02688ff27ee459e8a6ce672a2f7d64a2021-05-01T00:00:00Zhttps://doi.org/10.1111/jvim.16095https://doaj.org/toc/0891-6640https://doaj.org/toc/1939-1676Abstract Background Endocrinopathic laminitis develops in association with insulin dysregulation, but the role of insulin in the pathogenesis remains unclear. Hyperinsulinemia can cause hypoaminoacidemia, which is associated with integumentary lesions in other species and therefore warrants investigation as a potential mechanism in laminitis. Objective Evaluate plasma amino acid concentrations in the euglycemic‐hyperinsulinemic clamp (EHC) and prolonged glucose infusion (PGI) laminitis models. Animals Sixteen Standardbred horses. Methods Prospective experimental study. Plasma amino acid concentrations were measured in samples collected every 6 hours from horses that underwent a 48‐hour EHC (n = 8) or 66‐hour PGI (n = 8) after a 24‐ or 6‐hour baseline period in EHC and PGI groups, respectively. Results Fifteen of the 20 measured amino acid concentrations decreased over time in both EHC and PGI horses (P < 0.001). The median percentage change from baseline for these amino acids was: histidine (EHC: 41.5%; PGI: 43.9%), glutamine (EHC: 51.8%; PGI: 35.3%), arginine (EHC: 51.4%; PGI: 41%), glutamic acid (EHC: 52.4%; PGI: 31.7%), threonine (EHC: 62.8%; PGI: 25.2%), alanine (EHC: 48.9%; PGI: 19.5%), proline (EHC: 56.2%; PGI: 30.3%), cystine (EHC: 34.9%; PGI: 31.2%), lysine (EHC: 46.4%; PGI: 27.8%), tyrosine (EHC: 27.5%; PGI: 16.9%), methionine (EHC: 69.3%; PGI: 50.8%), valine (EHC: 50.8%; PGI: 34.4%), isoleucine (EHC: 60.8%; PGI: 38.7%), leucine (EHC: 48.2%; PGI: 36.6%), and phenylalanine (EHC: 16.6%; PGI: 12.1%). Conclusions and Clinical Importance Hypoaminoacidemia develops in EHC and PGI laminitis models. The role of hypoaminoacidemia in the development of hyperinsulinemia‐associated laminitis warrants further investigation.Simon M. StokesDarko StefanovskiFrançois‐René BertinCarlos E. Medina‐TorresJames K. BelknapAndrew W. vanEpsWileyarticleamino acidsendocrinopathichorsehypoaminoacidemiainsulinVeterinary medicineSF600-1100ENJournal of Veterinary Internal Medicine, Vol 35, Iss 3, Pp 1589-1596 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	amino acids endocrinopathic horse hypoaminoacidemia insulin Veterinary medicine SF600-1100
spellingShingle	amino acids endocrinopathic horse hypoaminoacidemia insulin Veterinary medicine SF600-1100 Simon M. Stokes Darko Stefanovski François‐René Bertin Carlos E. Medina‐Torres James K. Belknap Andrew W. vanEps Plasma amino acid concentrations during experimental hyperinsulinemia in 2 laminitis models
description	Abstract Background Endocrinopathic laminitis develops in association with insulin dysregulation, but the role of insulin in the pathogenesis remains unclear. Hyperinsulinemia can cause hypoaminoacidemia, which is associated with integumentary lesions in other species and therefore warrants investigation as a potential mechanism in laminitis. Objective Evaluate plasma amino acid concentrations in the euglycemic‐hyperinsulinemic clamp (EHC) and prolonged glucose infusion (PGI) laminitis models. Animals Sixteen Standardbred horses. Methods Prospective experimental study. Plasma amino acid concentrations were measured in samples collected every 6 hours from horses that underwent a 48‐hour EHC (n = 8) or 66‐hour PGI (n = 8) after a 24‐ or 6‐hour baseline period in EHC and PGI groups, respectively. Results Fifteen of the 20 measured amino acid concentrations decreased over time in both EHC and PGI horses (P < 0.001). The median percentage change from baseline for these amino acids was: histidine (EHC: 41.5%; PGI: 43.9%), glutamine (EHC: 51.8%; PGI: 35.3%), arginine (EHC: 51.4%; PGI: 41%), glutamic acid (EHC: 52.4%; PGI: 31.7%), threonine (EHC: 62.8%; PGI: 25.2%), alanine (EHC: 48.9%; PGI: 19.5%), proline (EHC: 56.2%; PGI: 30.3%), cystine (EHC: 34.9%; PGI: 31.2%), lysine (EHC: 46.4%; PGI: 27.8%), tyrosine (EHC: 27.5%; PGI: 16.9%), methionine (EHC: 69.3%; PGI: 50.8%), valine (EHC: 50.8%; PGI: 34.4%), isoleucine (EHC: 60.8%; PGI: 38.7%), leucine (EHC: 48.2%; PGI: 36.6%), and phenylalanine (EHC: 16.6%; PGI: 12.1%). Conclusions and Clinical Importance Hypoaminoacidemia develops in EHC and PGI laminitis models. The role of hypoaminoacidemia in the development of hyperinsulinemia‐associated laminitis warrants further investigation.
format	article
author	Simon M. Stokes Darko Stefanovski François‐René Bertin Carlos E. Medina‐Torres James K. Belknap Andrew W. vanEps
author_facet	Simon M. Stokes Darko Stefanovski François‐René Bertin Carlos E. Medina‐Torres James K. Belknap Andrew W. vanEps
author_sort	Simon M. Stokes
title	Plasma amino acid concentrations during experimental hyperinsulinemia in 2 laminitis models
title_short	Plasma amino acid concentrations during experimental hyperinsulinemia in 2 laminitis models
title_full	Plasma amino acid concentrations during experimental hyperinsulinemia in 2 laminitis models
title_fullStr	Plasma amino acid concentrations during experimental hyperinsulinemia in 2 laminitis models
title_full_unstemmed	Plasma amino acid concentrations during experimental hyperinsulinemia in 2 laminitis models
title_sort	plasma amino acid concentrations during experimental hyperinsulinemia in 2 laminitis models
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/e02688ff27ee459e8a6ce672a2f7d64a
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Plasma amino acid concentrations during experimental hyperinsulinemia in 2 laminitis models

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