Network Pharmacology Study and Experimental Confirmation Revealing the Ameliorative Effects of Decursin on Chemotherapy-Induced Alopecia

Decursin, a pyranocoumarin compound from the root of <i>Angelica gigas</i> Nakai as a main constituent, has been reported to have various biological activities, including anti-inflammatory, anticancer, and antioxidant effects. This study aimed to predict and confirm the pharmacological r...

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Autores principales:	Mi Hye Kim, Sang Jun Park, Woong Mo Yang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Decursin chemotherapy-induced alopecia hair follicles network pharmacology Medicine R Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/e02883e525894ccf85390fbf5dd92d9a
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spelling	oai:doaj.org-article:e02883e525894ccf85390fbf5dd92d9a2021-11-25T18:39:44ZNetwork Pharmacology Study and Experimental Confirmation Revealing the Ameliorative Effects of Decursin on Chemotherapy-Induced Alopecia10.3390/ph141111501424-8247https://doaj.org/article/e02883e525894ccf85390fbf5dd92d9a2021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8247/14/11/1150https://doaj.org/toc/1424-8247Decursin, a pyranocoumarin compound from the root of <i>Angelica gigas</i> Nakai as a main constituent, has been reported to have various biological activities, including anti-inflammatory, anticancer, and antioxidant effects. This study aimed to predict and confirm the pharmacological relevance of Decursin on chemotherapy-induced alopecia (CIA) with the underlying molecular mechanisms. Decursin-targeted genes were compared with the gene set of alopecia and investigated through functional enrichment analysis. CIA was induced in C57BL/6J mice by injection of cyclophosphamide, and 1, 10, and 100 μM of Decursin were topically treated to depilated dorsal skin. KGF<sup>+</sup> expression was detected in the dorsal skin tissues. Based on the predicted results, caspase, PIK3/AKT, and MAPKs protein expressions by Decursin were analyzed in the TNF-α-induced keratinocytes. The Decursin network had 60.20% overlapped genes with the network of alopecia. Biological processes, such as cellular response to chemical stimulus, apoptosis, PI3K-AKT signaling pathway, and MAPK signaling pathway, were derived from the Decursin network. In the Decursin-treated skin, there was morphological hair growth and histological restoration of hair follicles in the CIA mice. The KGF<sup>+</sup> fluorescence and protein expressions were significantly increased by Decursin treatment. In addition, caspase-3, -7, and -8 expressions, induced by TNF-α, were dose-dependently decreased along with the inhibition of PI3K, AKT, ERK, and p38 expressions in Decursin-treated keratinocytes. These findings indicated that Decursin would be a potent therapeutic option for hair loss, in response to chemotherapy.Mi Hye KimSang Jun ParkWoong Mo YangMDPI AGarticleDecursinchemotherapy-induced alopeciahair folliclesnetwork pharmacologyMedicineRPharmacy and materia medicaRS1-441ENPharmaceuticals, Vol 14, Iss 1150, p 1150 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Decursin chemotherapy-induced alopecia hair follicles network pharmacology Medicine R Pharmacy and materia medica RS1-441
spellingShingle	Decursin chemotherapy-induced alopecia hair follicles network pharmacology Medicine R Pharmacy and materia medica RS1-441 Mi Hye Kim Sang Jun Park Woong Mo Yang Network Pharmacology Study and Experimental Confirmation Revealing the Ameliorative Effects of Decursin on Chemotherapy-Induced Alopecia
description	Decursin, a pyranocoumarin compound from the root of <i>Angelica gigas</i> Nakai as a main constituent, has been reported to have various biological activities, including anti-inflammatory, anticancer, and antioxidant effects. This study aimed to predict and confirm the pharmacological relevance of Decursin on chemotherapy-induced alopecia (CIA) with the underlying molecular mechanisms. Decursin-targeted genes were compared with the gene set of alopecia and investigated through functional enrichment analysis. CIA was induced in C57BL/6J mice by injection of cyclophosphamide, and 1, 10, and 100 μM of Decursin were topically treated to depilated dorsal skin. KGF<sup>+</sup> expression was detected in the dorsal skin tissues. Based on the predicted results, caspase, PIK3/AKT, and MAPKs protein expressions by Decursin were analyzed in the TNF-α-induced keratinocytes. The Decursin network had 60.20% overlapped genes with the network of alopecia. Biological processes, such as cellular response to chemical stimulus, apoptosis, PI3K-AKT signaling pathway, and MAPK signaling pathway, were derived from the Decursin network. In the Decursin-treated skin, there was morphological hair growth and histological restoration of hair follicles in the CIA mice. The KGF<sup>+</sup> fluorescence and protein expressions were significantly increased by Decursin treatment. In addition, caspase-3, -7, and -8 expressions, induced by TNF-α, were dose-dependently decreased along with the inhibition of PI3K, AKT, ERK, and p38 expressions in Decursin-treated keratinocytes. These findings indicated that Decursin would be a potent therapeutic option for hair loss, in response to chemotherapy.
format	article
author	Mi Hye Kim Sang Jun Park Woong Mo Yang
author_facet	Mi Hye Kim Sang Jun Park Woong Mo Yang
author_sort	Mi Hye Kim
title	Network Pharmacology Study and Experimental Confirmation Revealing the Ameliorative Effects of Decursin on Chemotherapy-Induced Alopecia
title_short	Network Pharmacology Study and Experimental Confirmation Revealing the Ameliorative Effects of Decursin on Chemotherapy-Induced Alopecia
title_full	Network Pharmacology Study and Experimental Confirmation Revealing the Ameliorative Effects of Decursin on Chemotherapy-Induced Alopecia
title_fullStr	Network Pharmacology Study and Experimental Confirmation Revealing the Ameliorative Effects of Decursin on Chemotherapy-Induced Alopecia
title_full_unstemmed	Network Pharmacology Study and Experimental Confirmation Revealing the Ameliorative Effects of Decursin on Chemotherapy-Induced Alopecia
title_sort	network pharmacology study and experimental confirmation revealing the ameliorative effects of decursin on chemotherapy-induced alopecia
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e02883e525894ccf85390fbf5dd92d9a
work_keys_str_mv	AT mihyekim networkpharmacologystudyandexperimentalconfirmationrevealingtheameliorativeeffectsofdecursinonchemotherapyinducedalopecia AT sangjunpark networkpharmacologystudyandexperimentalconfirmationrevealingtheameliorativeeffectsofdecursinonchemotherapyinducedalopecia AT woongmoyang networkpharmacologystudyandexperimentalconfirmationrevealingtheameliorativeeffectsofdecursinonchemotherapyinducedalopecia
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Network Pharmacology Study and Experimental Confirmation Revealing the Ameliorative Effects of Decursin on Chemotherapy-Induced Alopecia

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