Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogens

The nucleotidyl cyclase toxin exoenzyme Y (ExoY), which is secreted by the human pathogens Pseudomonas aeruginosa and Vibrio vulnificus is activated by actin. Here, the authors present the cryo-EM structures of PaExoY bound to F-actin and VvExoY in complex with G-actin-profilin. These structures tog...

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Autores principales:	Alexander Belyy, Felipe Merino, Undine Mechold, Stefan Raunser
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e02948cd5c5f46128fc092474e87ab6c
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spelling	oai:doaj.org-article:e02948cd5c5f46128fc092474e87ab6c2021-11-21T12:35:57ZMechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogens10.1038/s41467-021-26889-22041-1723https://doaj.org/article/e02948cd5c5f46128fc092474e87ab6c2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-26889-2https://doaj.org/toc/2041-1723The nucleotidyl cyclase toxin exoenzyme Y (ExoY), which is secreted by the human pathogens Pseudomonas aeruginosa and Vibrio vulnificus is activated by actin. Here, the authors present the cryo-EM structures of PaExoY bound to F-actin and VvExoY in complex with G-actin-profilin. These structures together with molecular dynamics simulations and enzymatic assays provide insights into the activation mechanism for both bacterial cyclase toxin families that interact with either F- or G-actin.Alexander BelyyFelipe MerinoUndine MecholdStefan RaunserNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Alexander Belyy Felipe Merino Undine Mechold Stefan Raunser Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogens
description	The nucleotidyl cyclase toxin exoenzyme Y (ExoY), which is secreted by the human pathogens Pseudomonas aeruginosa and Vibrio vulnificus is activated by actin. Here, the authors present the cryo-EM structures of PaExoY bound to F-actin and VvExoY in complex with G-actin-profilin. These structures together with molecular dynamics simulations and enzymatic assays provide insights into the activation mechanism for both bacterial cyclase toxin families that interact with either F- or G-actin.
format	article
author	Alexander Belyy Felipe Merino Undine Mechold Stefan Raunser
author_facet	Alexander Belyy Felipe Merino Undine Mechold Stefan Raunser
author_sort	Alexander Belyy
title	Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogens
title_short	Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogens
title_full	Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogens
title_fullStr	Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogens
title_full_unstemmed	Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogens
title_sort	mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e02948cd5c5f46128fc092474e87ab6c
work_keys_str_mv	AT alexanderbelyy mechanismofactindependentactivationofnucleotidylcyclasetoxinsfrombacterialhumanpathogens AT felipemerino mechanismofactindependentactivationofnucleotidylcyclasetoxinsfrombacterialhumanpathogens AT undinemechold mechanismofactindependentactivationofnucleotidylcyclasetoxinsfrombacterialhumanpathogens AT stefanraunser mechanismofactindependentactivationofnucleotidylcyclasetoxinsfrombacterialhumanpathogens
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogens

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