Preparation and properties of BSA-loaded microspheres based on multi-(amino acid) copolymer for protein delivery

Xingtao Chen,1 Guoyue Lv,1 Jue Zhang,2 Songchao Tang,2 Yonggang Yan,1 Zhaoying Wu,2 Jiacan Su,2 Jie Wei2 1College of Physical Science and Technology, Sichuan University, Chengdu, 2Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shang...

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Autores principales:	Chen X, Lv G, Zhang J, Tang S, Yan Y, Wu Z, Su J, Wei J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e02982af4b574dcd9a4dcc0a2b2fc07c
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spelling	oai:doaj.org-article:e02982af4b574dcd9a4dcc0a2b2fc07c2021-12-02T07:28:30ZPreparation and properties of BSA-loaded microspheres based on multi-(amino acid) copolymer for protein delivery1178-2013https://doaj.org/article/e02982af4b574dcd9a4dcc0a2b2fc07c2014-05-01T00:00:00Zhttp://www.dovepress.com/preparation-and-properties-of-bsa-loaded-microspheres-based-on-multi-a-a16703https://doaj.org/toc/1178-2013 Xingtao Chen,1 Guoyue Lv,1 Jue Zhang,2 Songchao Tang,2 Yonggang Yan,1 Zhaoying Wu,2 Jiacan Su,2 Jie Wei2 1College of Physical Science and Technology, Sichuan University, Chengdu, 2Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People’s Republic of China Abstract: A multi-(amino acid) copolymer (MAC) based on ω-aminocaproic acid, γ-aminobutyric acid, L-alanine, L-lysine, L-glutamate, and hydroxyproline was synthetized, and MAC microspheres encapsulating bovine serum albumin (BSA) were prepared by a double-emulsion solvent extraction method. The experimental results show that various preparation parameters including surfactant ratio of Tween 80 to Span 80, surfactant concentration, benzyl alcohol in the external water phase, and polymer concentration had obvious effects on the particle size, morphology, and encapsulation efficiency of the BSA-loaded microspheres. The sizes of BSA-loaded microspheres ranged from 60.2 µm to 79.7 µm, showing different degrees of porous structure. The encapsulation efficiency of BSA-loaded microspheres also ranged from 38.8% to 50.8%. BSA release from microspheres showed the classic biphasic profile, which was governed by diffusion and polymer erosion. The initial burst release of BSA from microspheres at the first week followed by constant slow release for the next 7 weeks were observed. BSA-loaded microspheres could degrade gradually in phosphate buffered saline buffer with pH value maintained at around 7.1 during 8 weeks incubation, suggesting that microsphere degradation did not cause a dramatic pH drop in phosphate buffered saline buffer because no acidic degradation products were released from the microspheres. Therefore, the MAC microspheres might have great potential as carriers for protein delivery. Keywords: poly (amino acid) copolymer, release, degradationChen XLv GZhang JTang SYan YWu ZSu JWei JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1957-1965 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Chen X Lv G Zhang J Tang S Yan Y Wu Z Su J Wei J Preparation and properties of BSA-loaded microspheres based on multi-(amino acid) copolymer for protein delivery
description	Xingtao Chen,1 Guoyue Lv,1 Jue Zhang,2 Songchao Tang,2 Yonggang Yan,1 Zhaoying Wu,2 Jiacan Su,2 Jie Wei2 1College of Physical Science and Technology, Sichuan University, Chengdu, 2Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People’s Republic of China Abstract: A multi-(amino acid) copolymer (MAC) based on ω-aminocaproic acid, γ-aminobutyric acid, L-alanine, L-lysine, L-glutamate, and hydroxyproline was synthetized, and MAC microspheres encapsulating bovine serum albumin (BSA) were prepared by a double-emulsion solvent extraction method. The experimental results show that various preparation parameters including surfactant ratio of Tween 80 to Span 80, surfactant concentration, benzyl alcohol in the external water phase, and polymer concentration had obvious effects on the particle size, morphology, and encapsulation efficiency of the BSA-loaded microspheres. The sizes of BSA-loaded microspheres ranged from 60.2 µm to 79.7 µm, showing different degrees of porous structure. The encapsulation efficiency of BSA-loaded microspheres also ranged from 38.8% to 50.8%. BSA release from microspheres showed the classic biphasic profile, which was governed by diffusion and polymer erosion. The initial burst release of BSA from microspheres at the first week followed by constant slow release for the next 7 weeks were observed. BSA-loaded microspheres could degrade gradually in phosphate buffered saline buffer with pH value maintained at around 7.1 during 8 weeks incubation, suggesting that microsphere degradation did not cause a dramatic pH drop in phosphate buffered saline buffer because no acidic degradation products were released from the microspheres. Therefore, the MAC microspheres might have great potential as carriers for protein delivery. Keywords: poly (amino acid) copolymer, release, degradation
format	article
author	Chen X Lv G Zhang J Tang S Yan Y Wu Z Su J Wei J
author_facet	Chen X Lv G Zhang J Tang S Yan Y Wu Z Su J Wei J
author_sort	Chen X
title	Preparation and properties of BSA-loaded microspheres based on multi-(amino acid) copolymer for protein delivery
title_short	Preparation and properties of BSA-loaded microspheres based on multi-(amino acid) copolymer for protein delivery
title_full	Preparation and properties of BSA-loaded microspheres based on multi-(amino acid) copolymer for protein delivery
title_fullStr	Preparation and properties of BSA-loaded microspheres based on multi-(amino acid) copolymer for protein delivery
title_full_unstemmed	Preparation and properties of BSA-loaded microspheres based on multi-(amino acid) copolymer for protein delivery
title_sort	preparation and properties of bsa-loaded microspheres based on multi-(amino acid) copolymer for protein delivery
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/e02982af4b574dcd9a4dcc0a2b2fc07c
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Preparation and properties of BSA-loaded microspheres based on multi-(amino acid) copolymer for protein delivery

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