Comparing the Expressions of Vitamin D Receptor, Cell Proliferation, and Apoptosis in Gastric Mucosa With Gastritis, Intestinal Metaplasia, or Adenocarcinoma Change

Background: This study aimed to compare the expression of vitamin D receptor (VDR), cell proliferation, and apoptosis in the gastric mucosa of patients with gastritis, intestinal metaplasia (IM), and adenocarcinoma using artificial intelligence.Material and Methods: This study retrospectively enroll...

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Autores principales:	Li-Wei Chen, Liang-Che Chang, Chung-Ching Hua, Tzu-Chien Cheng, Chin-Chan Lee
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	adenocarcinoma stomach neoplasm cell proliferation apoptosis artificial intelligence Ki67 antigen Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e02bc8fd82974885ba818ac4418139ab
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spelling	oai:doaj.org-article:e02bc8fd82974885ba818ac4418139ab2021-11-22T06:29:16ZComparing the Expressions of Vitamin D Receptor, Cell Proliferation, and Apoptosis in Gastric Mucosa With Gastritis, Intestinal Metaplasia, or Adenocarcinoma Change2296-858X10.3389/fmed.2021.766061https://doaj.org/article/e02bc8fd82974885ba818ac4418139ab2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmed.2021.766061/fullhttps://doaj.org/toc/2296-858XBackground: This study aimed to compare the expression of vitamin D receptor (VDR), cell proliferation, and apoptosis in the gastric mucosa of patients with gastritis, intestinal metaplasia (IM), and adenocarcinoma using artificial intelligence.Material and Methods: This study retrospectively enrolled patients at the Keelung Chang Gung Memorial Hospital from November of 2016 to June, 2017, who were diagnosed with gastric adenocarcinoma. The inclusion criteria were patients' pathologic reports that revealed all compartments of Helicobacter pylori infection, gastritis, IM, and adenocarcinoma simultaneously in the same gastric sample. Tissue slides after immunohistochemical (IHC) staining were transformed into digital images using a scanner and counted using computer software (QuPath and ImageJ). IHC staining included PA1-711 antibody for VDR, Ki67 antigen for proliferation, and M30 antibody CK18 for apoptosis.Results: Twenty-nine patients were included in the IHC staining quantitative analysis. The mean age was 69.1 ± 11.3 y/o. Most (25/29, 86.2%) patients had poorly differentiated adenocarcinoma. The mean expression of Ki67 and CK18 increased progressively from gastritis and IM to adenocarcinoma, with statistical significance (P < 0.05). VDR expression did not correlate with Ki67 or CK18 expression. Survival time was only correlated with tumor stage (correlation coefficient = −0.423, P value < 0.05), but was not correlated with the expression of VDR, Ki67, and CK18.Conclusion: Ki67 expression and CK18 expression progressively increased in the areas of gastritis, IM, and adenocarcinoma. No correlation between VDR expression and Ki67 or CK18 expression was found in this study.Li-Wei ChenLi-Wei ChenLiang-Che ChangChung-Ching HuaTzu-Chien ChengChin-Chan LeeChin-Chan LeeFrontiers Media S.A.articleadenocarcinomastomach neoplasmcell proliferationapoptosisartificial intelligenceKi67 antigenMedicine (General)R5-920ENFrontiers in Medicine, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adenocarcinoma stomach neoplasm cell proliferation apoptosis artificial intelligence Ki67 antigen Medicine (General) R5-920
spellingShingle	adenocarcinoma stomach neoplasm cell proliferation apoptosis artificial intelligence Ki67 antigen Medicine (General) R5-920 Li-Wei Chen Li-Wei Chen Liang-Che Chang Chung-Ching Hua Tzu-Chien Cheng Chin-Chan Lee Chin-Chan Lee Comparing the Expressions of Vitamin D Receptor, Cell Proliferation, and Apoptosis in Gastric Mucosa With Gastritis, Intestinal Metaplasia, or Adenocarcinoma Change
description	Background: This study aimed to compare the expression of vitamin D receptor (VDR), cell proliferation, and apoptosis in the gastric mucosa of patients with gastritis, intestinal metaplasia (IM), and adenocarcinoma using artificial intelligence.Material and Methods: This study retrospectively enrolled patients at the Keelung Chang Gung Memorial Hospital from November of 2016 to June, 2017, who were diagnosed with gastric adenocarcinoma. The inclusion criteria were patients' pathologic reports that revealed all compartments of Helicobacter pylori infection, gastritis, IM, and adenocarcinoma simultaneously in the same gastric sample. Tissue slides after immunohistochemical (IHC) staining were transformed into digital images using a scanner and counted using computer software (QuPath and ImageJ). IHC staining included PA1-711 antibody for VDR, Ki67 antigen for proliferation, and M30 antibody CK18 for apoptosis.Results: Twenty-nine patients were included in the IHC staining quantitative analysis. The mean age was 69.1 ± 11.3 y/o. Most (25/29, 86.2%) patients had poorly differentiated adenocarcinoma. The mean expression of Ki67 and CK18 increased progressively from gastritis and IM to adenocarcinoma, with statistical significance (P < 0.05). VDR expression did not correlate with Ki67 or CK18 expression. Survival time was only correlated with tumor stage (correlation coefficient = −0.423, P value < 0.05), but was not correlated with the expression of VDR, Ki67, and CK18.Conclusion: Ki67 expression and CK18 expression progressively increased in the areas of gastritis, IM, and adenocarcinoma. No correlation between VDR expression and Ki67 or CK18 expression was found in this study.
format	article
author	Li-Wei Chen Li-Wei Chen Liang-Che Chang Chung-Ching Hua Tzu-Chien Cheng Chin-Chan Lee Chin-Chan Lee
author_facet	Li-Wei Chen Li-Wei Chen Liang-Che Chang Chung-Ching Hua Tzu-Chien Cheng Chin-Chan Lee Chin-Chan Lee
author_sort	Li-Wei Chen
title	Comparing the Expressions of Vitamin D Receptor, Cell Proliferation, and Apoptosis in Gastric Mucosa With Gastritis, Intestinal Metaplasia, or Adenocarcinoma Change
title_short	Comparing the Expressions of Vitamin D Receptor, Cell Proliferation, and Apoptosis in Gastric Mucosa With Gastritis, Intestinal Metaplasia, or Adenocarcinoma Change
title_full	Comparing the Expressions of Vitamin D Receptor, Cell Proliferation, and Apoptosis in Gastric Mucosa With Gastritis, Intestinal Metaplasia, or Adenocarcinoma Change
title_fullStr	Comparing the Expressions of Vitamin D Receptor, Cell Proliferation, and Apoptosis in Gastric Mucosa With Gastritis, Intestinal Metaplasia, or Adenocarcinoma Change
title_full_unstemmed	Comparing the Expressions of Vitamin D Receptor, Cell Proliferation, and Apoptosis in Gastric Mucosa With Gastritis, Intestinal Metaplasia, or Adenocarcinoma Change
title_sort	comparing the expressions of vitamin d receptor, cell proliferation, and apoptosis in gastric mucosa with gastritis, intestinal metaplasia, or adenocarcinoma change
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e02bc8fd82974885ba818ac4418139ab
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Comparing the Expressions of Vitamin D Receptor, Cell Proliferation, and Apoptosis in Gastric Mucosa With Gastritis, Intestinal Metaplasia, or Adenocarcinoma Change

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