MicroRNA-485-5p inhibits glioblastoma progression by suppressing E2F transcription factor 1 under cisplatin treatment

Cisplatin (CDDP) has been widely used for glioblastoma treatment. miR-485-5p and E2F transcription factor 1 (E2F1) dysfunction has been reported in glioblastoma. Nonetheless, whether CDDP affects glioblastoma progression via the miR-485-5p-E2F1 axis requires investigation. The expression of miR-485-...

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Autores principales:	Conggang Huang, Lan Ma, Faliang Duan, Ruixue Li, Yanguo Zhang, Yuan Wang, Ming Luo, Zhuqiang He, Zhihua Luo
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	cisplatin mir-485-5p e2f1 glioblastoma cell growth Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/e03020a3b952492a8815706f5c6c7245
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spelling	oai:doaj.org-article:e03020a3b952492a8815706f5c6c72452021-11-04T15:51:53ZMicroRNA-485-5p inhibits glioblastoma progression by suppressing E2F transcription factor 1 under cisplatin treatment2165-59792165-598710.1080/21655979.2021.1982269https://doaj.org/article/e03020a3b952492a8815706f5c6c72452021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1982269https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Cisplatin (CDDP) has been widely used for glioblastoma treatment. miR-485-5p and E2F transcription factor 1 (E2F1) dysfunction has been reported in glioblastoma. Nonetheless, whether CDDP affects glioblastoma progression via the miR-485-5p-E2F1 axis requires investigation. The expression of miR-485-5p and E2F1 was investigated by quantitative real-time polymerase chain reaction or western blotting in glioblastoma tissues and cell lines. The interaction between miR-485-5p and E2F1 was confirmed using a luciferase assay. The malignancy of glioblastoma was detected using Cell Counting Kit-8, bromodeoxyuridine (BrdU), cell adhesion, flow cytometry, and transwell assays. We identified miR-485-5p downregulation and E2F1 upregulation in glioblastoma, and miR-485-5p inhibited cell growth and elevated cell apoptosis in glioblastoma cells after CDDP treatment. Moreover, miR-485-5p targeting E2F1 repressed cell growth and improved cell apoptosis in glioblastoma cells after CDDP treatment. Our study revealed that CDDP retarded glioblastoma cell development via the miR-485-5p-E2F1 axis, which may be a new direction for glioblastoma therapy.Conggang HuangLan MaFaliang DuanRuixue LiYanguo ZhangYuan WangMing LuoZhuqiang HeZhihua LuoTaylor & Francis Grouparticlecisplatinmir-485-5pe2f1glioblastomacell growthBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 8020-8030 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cisplatin mir-485-5p e2f1 glioblastoma cell growth Biotechnology TP248.13-248.65
spellingShingle	cisplatin mir-485-5p e2f1 glioblastoma cell growth Biotechnology TP248.13-248.65 Conggang Huang Lan Ma Faliang Duan Ruixue Li Yanguo Zhang Yuan Wang Ming Luo Zhuqiang He Zhihua Luo MicroRNA-485-5p inhibits glioblastoma progression by suppressing E2F transcription factor 1 under cisplatin treatment
description	Cisplatin (CDDP) has been widely used for glioblastoma treatment. miR-485-5p and E2F transcription factor 1 (E2F1) dysfunction has been reported in glioblastoma. Nonetheless, whether CDDP affects glioblastoma progression via the miR-485-5p-E2F1 axis requires investigation. The expression of miR-485-5p and E2F1 was investigated by quantitative real-time polymerase chain reaction or western blotting in glioblastoma tissues and cell lines. The interaction between miR-485-5p and E2F1 was confirmed using a luciferase assay. The malignancy of glioblastoma was detected using Cell Counting Kit-8, bromodeoxyuridine (BrdU), cell adhesion, flow cytometry, and transwell assays. We identified miR-485-5p downregulation and E2F1 upregulation in glioblastoma, and miR-485-5p inhibited cell growth and elevated cell apoptosis in glioblastoma cells after CDDP treatment. Moreover, miR-485-5p targeting E2F1 repressed cell growth and improved cell apoptosis in glioblastoma cells after CDDP treatment. Our study revealed that CDDP retarded glioblastoma cell development via the miR-485-5p-E2F1 axis, which may be a new direction for glioblastoma therapy.
format	article
author	Conggang Huang Lan Ma Faliang Duan Ruixue Li Yanguo Zhang Yuan Wang Ming Luo Zhuqiang He Zhihua Luo
author_facet	Conggang Huang Lan Ma Faliang Duan Ruixue Li Yanguo Zhang Yuan Wang Ming Luo Zhuqiang He Zhihua Luo
author_sort	Conggang Huang
title	MicroRNA-485-5p inhibits glioblastoma progression by suppressing E2F transcription factor 1 under cisplatin treatment
title_short	MicroRNA-485-5p inhibits glioblastoma progression by suppressing E2F transcription factor 1 under cisplatin treatment
title_full	MicroRNA-485-5p inhibits glioblastoma progression by suppressing E2F transcription factor 1 under cisplatin treatment
title_fullStr	MicroRNA-485-5p inhibits glioblastoma progression by suppressing E2F transcription factor 1 under cisplatin treatment
title_full_unstemmed	MicroRNA-485-5p inhibits glioblastoma progression by suppressing E2F transcription factor 1 under cisplatin treatment
title_sort	microrna-485-5p inhibits glioblastoma progression by suppressing e2f transcription factor 1 under cisplatin treatment
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/e03020a3b952492a8815706f5c6c7245
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MicroRNA-485-5p inhibits glioblastoma progression by suppressing E2F transcription factor 1 under cisplatin treatment

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