Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes

Global seed vaults are important, as they conserve plant genetic resources for future breeding to improve crop yield and quality and to overcome biotic and abiotic stresses. However, little is known about the impact of standard storage procedures, such as seed drying and cold storage on the seed bac...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ankush Chandel, Ross Mann, Jatinder Kaur, Sally Norton, Jacqueline Edwards, German Spangenberg, Timothy Sawbridge
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	seed vault storage strategies seed bacterial microbiomes conservation culturability Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e03ad3bf0437494e8219a28c1090f555
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e03ad3bf0437494e8219a28c1090f555
record_format	dspace
spelling	oai:doaj.org-article:e03ad3bf0437494e8219a28c1090f5552021-12-03T07:14:51ZImplications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes1664-302X10.3389/fmicb.2021.784796https://doaj.org/article/e03ad3bf0437494e8219a28c1090f5552021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.784796/fullhttps://doaj.org/toc/1664-302XGlobal seed vaults are important, as they conserve plant genetic resources for future breeding to improve crop yield and quality and to overcome biotic and abiotic stresses. However, little is known about the impact of standard storage procedures, such as seed drying and cold storage on the seed bacterial community, and the ability to recover seed-associated bacteria after storage. In this study, soybean [Glycine max (L.) Merr.] seeds were analyzed to characterize changes in the bacterial community composition and culturability under varying storage conditions. The G. max bacterial microbiome was analyzed from undried seed, dried seed, and seed stored for 0, 3, 6, and 14months. Storage temperatures consisted of −20°C, 4°C, and room temperature (RT), with −20°C being commonly used in seed storage vaults globally. The seed microbiome of G. max was dominated by Gammaproteobacteria under all conditions. Undried seed was dominated by Pantoea (33.9%) and Pseudomonas (51.1%); however, following drying, the abundance of Pseudomonas declined significantly (0.9%), Pantoea increased significantly (73.6%), and four genera previously identified including Pajaroellobacter, Nesterenkonia, env.OPS_17, and Acidibacter were undetectable. Subsequent storage at RT, 4, or −20°C maintained high-abundance Genera at the majority of time points, although RT caused greater fluctuations in abundances. For many of the low-abundance Genera, storage at −20°C resulted in their gradual disappearance, whereas storage at 4°C or RT resulted in their more rapid disappearance. The changes in seed bacterial composition were reflected by cultured bacterial taxa obtained from the stored G. max seed. The main taxa were largely culturable and had similar relative abundance, while many, but not all, of the low-abundance taxa were also culturable. Overall, these results indicate that the initial seed drying affects the seed bacterial composition, suggesting that microbial isolation prior to seed drying is recommended to conserve these microbes. The standard seed storage condition of −20°C is most suitable for conservation of the bacterial seed microbiome, as this storage temperature slows down the loss of seed bacterial diversity over longer time periods, particularly low-abundance taxa.Ankush ChandelAnkush ChandelRoss MannJatinder KaurSally NortonJacqueline EdwardsJacqueline EdwardsGerman SpangenbergGerman SpangenbergTimothy SawbridgeTimothy SawbridgeFrontiers Media S.A.articleseed vaultstorage strategiesseed bacterial microbiomesconservationculturabilityMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	seed vault storage strategies seed bacterial microbiomes conservation culturability Microbiology QR1-502
spellingShingle	seed vault storage strategies seed bacterial microbiomes conservation culturability Microbiology QR1-502 Ankush Chandel Ankush Chandel Ross Mann Jatinder Kaur Sally Norton Jacqueline Edwards Jacqueline Edwards German Spangenberg German Spangenberg Timothy Sawbridge Timothy Sawbridge Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes
description	Global seed vaults are important, as they conserve plant genetic resources for future breeding to improve crop yield and quality and to overcome biotic and abiotic stresses. However, little is known about the impact of standard storage procedures, such as seed drying and cold storage on the seed bacterial community, and the ability to recover seed-associated bacteria after storage. In this study, soybean [Glycine max (L.) Merr.] seeds were analyzed to characterize changes in the bacterial community composition and culturability under varying storage conditions. The G. max bacterial microbiome was analyzed from undried seed, dried seed, and seed stored for 0, 3, 6, and 14months. Storage temperatures consisted of −20°C, 4°C, and room temperature (RT), with −20°C being commonly used in seed storage vaults globally. The seed microbiome of G. max was dominated by Gammaproteobacteria under all conditions. Undried seed was dominated by Pantoea (33.9%) and Pseudomonas (51.1%); however, following drying, the abundance of Pseudomonas declined significantly (0.9%), Pantoea increased significantly (73.6%), and four genera previously identified including Pajaroellobacter, Nesterenkonia, env.OPS_17, and Acidibacter were undetectable. Subsequent storage at RT, 4, or −20°C maintained high-abundance Genera at the majority of time points, although RT caused greater fluctuations in abundances. For many of the low-abundance Genera, storage at −20°C resulted in their gradual disappearance, whereas storage at 4°C or RT resulted in their more rapid disappearance. The changes in seed bacterial composition were reflected by cultured bacterial taxa obtained from the stored G. max seed. The main taxa were largely culturable and had similar relative abundance, while many, but not all, of the low-abundance taxa were also culturable. Overall, these results indicate that the initial seed drying affects the seed bacterial composition, suggesting that microbial isolation prior to seed drying is recommended to conserve these microbes. The standard seed storage condition of −20°C is most suitable for conservation of the bacterial seed microbiome, as this storage temperature slows down the loss of seed bacterial diversity over longer time periods, particularly low-abundance taxa.
format	article
author	Ankush Chandel Ankush Chandel Ross Mann Jatinder Kaur Sally Norton Jacqueline Edwards Jacqueline Edwards German Spangenberg German Spangenberg Timothy Sawbridge Timothy Sawbridge
author_facet	Ankush Chandel Ankush Chandel Ross Mann Jatinder Kaur Sally Norton Jacqueline Edwards Jacqueline Edwards German Spangenberg German Spangenberg Timothy Sawbridge Timothy Sawbridge
author_sort	Ankush Chandel
title	Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes
title_short	Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes
title_full	Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes
title_fullStr	Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes
title_full_unstemmed	Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes
title_sort	implications of seed vault storage strategies for conservation of seed bacterial microbiomes
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e03ad3bf0437494e8219a28c1090f555
work_keys_str_mv	AT ankushchandel implicationsofseedvaultstoragestrategiesforconservationofseedbacterialmicrobiomes AT ankushchandel implicationsofseedvaultstoragestrategiesforconservationofseedbacterialmicrobiomes AT rossmann implicationsofseedvaultstoragestrategiesforconservationofseedbacterialmicrobiomes AT jatinderkaur implicationsofseedvaultstoragestrategiesforconservationofseedbacterialmicrobiomes AT sallynorton implicationsofseedvaultstoragestrategiesforconservationofseedbacterialmicrobiomes AT jacquelineedwards implicationsofseedvaultstoragestrategiesforconservationofseedbacterialmicrobiomes AT jacquelineedwards implicationsofseedvaultstoragestrategiesforconservationofseedbacterialmicrobiomes AT germanspangenberg implicationsofseedvaultstoragestrategiesforconservationofseedbacterialmicrobiomes AT germanspangenberg implicationsofseedvaultstoragestrategiesforconservationofseedbacterialmicrobiomes AT timothysawbridge implicationsofseedvaultstoragestrategiesforconservationofseedbacterialmicrobiomes AT timothysawbridge implicationsofseedvaultstoragestrategiesforconservationofseedbacterialmicrobiomes
_version_	1718373845390327808

Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes

Ejemplares similares