Influence of cationic lipid concentration on properties of lipid–polymer hybrid nanospheres for gene delivery

Influence of cationic lipid concentration on properties of lipid–polymer hybrid nanospheres for gene delivery

Rajendran JC Bose,1,2 Yoshie Arai,1 Jong Chan Ahn,1 Hansoo Park,2 Soo-Hong Lee11Department of Biomedical Science, College of Life Science, CHA University, Seongnam, 2Department of Integrative Engineering, Chung-Ang University, Seoul, South Korea Abstract: Nanoparticles have been widely used for nonv...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Bose RJC, Arai Y, Ahn JC, Park H, Lee SH
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/e04f95a2e45a4a0f9c0deaa8590df00e
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:e04f95a2e45a4a0f9c0deaa8590df00e
record_format dspace
spelling oai:doaj.org-article:e04f95a2e45a4a0f9c0deaa8590df00e2021-12-02T02:21:11ZInfluence of cationic lipid concentration on properties of lipid–polymer hybrid nanospheres for gene delivery1178-2013https://doaj.org/article/e04f95a2e45a4a0f9c0deaa8590df00e2015-09-01T00:00:00Zhttps://www.dovepress.com/influence-of-cationic-lipid-concentration-on-properties-of-lipidndashp-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Rajendran JC Bose,1,2 Yoshie Arai,1 Jong Chan Ahn,1 Hansoo Park,2 Soo-Hong Lee11Department of Biomedical Science, College of Life Science, CHA University, Seongnam, 2Department of Integrative Engineering, Chung-Ang University, Seoul, South Korea Abstract: Nanoparticles have been widely used for nonviral gene delivery. Recently, cationic hybrid nanoparticles consisting of two different materials were suggested as a promising delivery vehicle. In this study, nanospheres with a poly(D,l-lactic-co-glycolic acid) (PLGA) core and cationic lipid shell were prepared, and the effect of cationic lipid concentrations on the properties of lipid polymer hybrid nanocarriers investigated. Lipid–polymer hybrid nanospheres (LPHNSs) were fabricated by the emulsion-solvent evaporation method using different concentrations of cationic lipids and characterized for size, surface charge, stability, plasmid DNA-binding capacity, cytotoxicity, and transfection efficiency. All LPHNSs had narrow size distribution with positive surface charges (ζ-potential 52–60 mV), and showed excellent plasmid DNA-binding capacity. In vitro cytotoxicity measurements with HEK293T, HeLa, HaCaT, and HepG2 cells also showed that LPHNSs exhibited less cytotoxicity than conventional transfection agents, such as Lipofectamine and polyethyleneimine–PLGA. As cationic lipid concentrations increased, the particle size of LPHNSs decreased while their ζ-potential increased. In addition, the in vitro transfection efficiency of LPHNSs increased as lipid concentration increased. Keywords: core–shell hybrid nanospheres, lipid concentration, surface modification, low cytotoxicity, transfection efficiencyBose RJCArai YAhn JCPark HLee SHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 5367-5382 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Bose RJC
Arai Y
Ahn JC
Park H
Lee SH
Influence of cationic lipid concentration on properties of lipid–polymer hybrid nanospheres for gene delivery
description Rajendran JC Bose,1,2 Yoshie Arai,1 Jong Chan Ahn,1 Hansoo Park,2 Soo-Hong Lee11Department of Biomedical Science, College of Life Science, CHA University, Seongnam, 2Department of Integrative Engineering, Chung-Ang University, Seoul, South Korea Abstract: Nanoparticles have been widely used for nonviral gene delivery. Recently, cationic hybrid nanoparticles consisting of two different materials were suggested as a promising delivery vehicle. In this study, nanospheres with a poly(D,l-lactic-co-glycolic acid) (PLGA) core and cationic lipid shell were prepared, and the effect of cationic lipid concentrations on the properties of lipid polymer hybrid nanocarriers investigated. Lipid–polymer hybrid nanospheres (LPHNSs) were fabricated by the emulsion-solvent evaporation method using different concentrations of cationic lipids and characterized for size, surface charge, stability, plasmid DNA-binding capacity, cytotoxicity, and transfection efficiency. All LPHNSs had narrow size distribution with positive surface charges (ζ-potential 52–60 mV), and showed excellent plasmid DNA-binding capacity. In vitro cytotoxicity measurements with HEK293T, HeLa, HaCaT, and HepG2 cells also showed that LPHNSs exhibited less cytotoxicity than conventional transfection agents, such as Lipofectamine and polyethyleneimine–PLGA. As cationic lipid concentrations increased, the particle size of LPHNSs decreased while their ζ-potential increased. In addition, the in vitro transfection efficiency of LPHNSs increased as lipid concentration increased. Keywords: core–shell hybrid nanospheres, lipid concentration, surface modification, low cytotoxicity, transfection efficiency
format article
author Bose RJC
Arai Y
Ahn JC
Park H
Lee SH
author_facet Bose RJC
Arai Y
Ahn JC
Park H
Lee SH
author_sort Bose RJC
title Influence of cationic lipid concentration on properties of lipid–polymer hybrid nanospheres for gene delivery
title_short Influence of cationic lipid concentration on properties of lipid–polymer hybrid nanospheres for gene delivery
title_full Influence of cationic lipid concentration on properties of lipid–polymer hybrid nanospheres for gene delivery
title_fullStr Influence of cationic lipid concentration on properties of lipid–polymer hybrid nanospheres for gene delivery
title_full_unstemmed Influence of cationic lipid concentration on properties of lipid–polymer hybrid nanospheres for gene delivery
title_sort influence of cationic lipid concentration on properties of lipid–polymer hybrid nanospheres for gene delivery
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/e04f95a2e45a4a0f9c0deaa8590df00e
work_keys_str_mv AT boserjc influenceofcationiclipidconcentrationonpropertiesoflipidndashpolymerhybridnanospheresforgenedelivery
AT araiy influenceofcationiclipidconcentrationonpropertiesoflipidndashpolymerhybridnanospheresforgenedelivery
AT ahnjc influenceofcationiclipidconcentrationonpropertiesoflipidndashpolymerhybridnanospheresforgenedelivery
AT parkh influenceofcationiclipidconcentrationonpropertiesoflipidndashpolymerhybridnanospheresforgenedelivery
AT leesh influenceofcationiclipidconcentrationonpropertiesoflipidndashpolymerhybridnanospheresforgenedelivery
_version_ 1718402560485752832

Ejemplares similares