Design principles governing chemomechanical coupling of kinesin

Abstract A systematic chemomechanical network model for the molecular motor kinesin is presented in this report. The network model is based on the nucleotide-dependent binding affinity of the heads to an microtubule (MT) and the asymmetries and similarities between the chemical transitions caused by...

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Autor principal:	Tomonari Sumi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e0518f00b30f48bf8d9f4d8fc9cb1d01
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spelling	oai:doaj.org-article:e0518f00b30f48bf8d9f4d8fc9cb1d012021-12-02T12:32:13ZDesign principles governing chemomechanical coupling of kinesin10.1038/s41598-017-01328-92045-2322https://doaj.org/article/e0518f00b30f48bf8d9f4d8fc9cb1d012017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01328-9https://doaj.org/toc/2045-2322Abstract A systematic chemomechanical network model for the molecular motor kinesin is presented in this report. The network model is based on the nucleotide-dependent binding affinity of the heads to an microtubule (MT) and the asymmetries and similarities between the chemical transitions caused by the intramolecular strain between the front and rear heads. The network model allows for multiple chemomechanical cycles and takes into account all possible mechanical transitions between states in which one head is strongly bound and the other head is weakly bound to an MT. The results obtained from the model show the ATP-concentration dependence of the dominant forward stepping cycle and support a gated rear head mechanism in which the forward step is controlled by ATP hydrolysis and the resulting ADP-bound state of the rear head when the ATP level is saturated. When the ATP level is saturated, the energy from ATP hydrolysis is used to concentrate the chemical transition flux to a force-generating state that can produce the power stroke. In contrast, when the ATP level is low, the hydrolysis energy is consumed to avoid states in which the leading head is weakly bound to an MT and to inhibit frequent backward steps upon loading.Tomonari SumiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomonari Sumi Design principles governing chemomechanical coupling of kinesin
description	Abstract A systematic chemomechanical network model for the molecular motor kinesin is presented in this report. The network model is based on the nucleotide-dependent binding affinity of the heads to an microtubule (MT) and the asymmetries and similarities between the chemical transitions caused by the intramolecular strain between the front and rear heads. The network model allows for multiple chemomechanical cycles and takes into account all possible mechanical transitions between states in which one head is strongly bound and the other head is weakly bound to an MT. The results obtained from the model show the ATP-concentration dependence of the dominant forward stepping cycle and support a gated rear head mechanism in which the forward step is controlled by ATP hydrolysis and the resulting ADP-bound state of the rear head when the ATP level is saturated. When the ATP level is saturated, the energy from ATP hydrolysis is used to concentrate the chemical transition flux to a force-generating state that can produce the power stroke. In contrast, when the ATP level is low, the hydrolysis energy is consumed to avoid states in which the leading head is weakly bound to an MT and to inhibit frequent backward steps upon loading.
format	article
author	Tomonari Sumi
author_facet	Tomonari Sumi
author_sort	Tomonari Sumi
title	Design principles governing chemomechanical coupling of kinesin
title_short	Design principles governing chemomechanical coupling of kinesin
title_full	Design principles governing chemomechanical coupling of kinesin
title_fullStr	Design principles governing chemomechanical coupling of kinesin
title_full_unstemmed	Design principles governing chemomechanical coupling of kinesin
title_sort	design principles governing chemomechanical coupling of kinesin
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e0518f00b30f48bf8d9f4d8fc9cb1d01
work_keys_str_mv	AT tomonarisumi designprinciplesgoverningchemomechanicalcouplingofkinesin
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Design principles governing chemomechanical coupling of kinesin

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