Proteomic analysis and qRT-PCR verification of temperature response to Arthrospira (Spirulina) platensis.

Arthrospira (Spirulina) platensis (ASP) is a representative filamentous, non-N2-fixing cyanobacterium that has great potential to enhance the food supply and possesses several valuable physiological features. ASP tolerates high and low temperatures along with highly alkaline and salty environments,...

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Autores principales:	Wang Huili, Zhao Xiaokai, Lin Meili, Randy A Dahlgren, Chen Wei, Zhou Jaiopeng, Xu Chengyang, Jin Chunlei, Xu Yi, Wang Xuedong, Ding Li, Bao Qiyu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e052696018f743458439e81ded94c935
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spelling	oai:doaj.org-article:e052696018f743458439e81ded94c9352021-11-18T08:42:08ZProteomic analysis and qRT-PCR verification of temperature response to Arthrospira (Spirulina) platensis.1932-620310.1371/journal.pone.0083485https://doaj.org/article/e052696018f743458439e81ded94c9352013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349519/?tool=EBIhttps://doaj.org/toc/1932-6203Arthrospira (Spirulina) platensis (ASP) is a representative filamentous, non-N2-fixing cyanobacterium that has great potential to enhance the food supply and possesses several valuable physiological features. ASP tolerates high and low temperatures along with highly alkaline and salty environments, and can strongly resist oxidation and irradiation. Based on genomic sequencing of ASP, we compared the protein expression profiles of this organism under different temperature conditions (15°C, 35°Cand 45°C) using 2-DE and peptide mass fingerprinting techniques. A total of 122 proteins having a significant differential expression response to temperature were retrieved. Of the positively expressed proteins, the homologies of 116 ASP proteins were found in Arthrospira (81 proteins in Arthrospira platensis str. Paraca and 35 in Arthrospira maxima CS-328). The other 6 proteins have high homology with other microorganisms. We classified the 122 differentially expressed positive proteins into 14 functions using the COG database, and characterized their respective KEGG metabolism pathways. The results demonstrated that these differentially expressed proteins are mainly involved in post-translational modification (protein turnover, chaperones), energy metabolism (photosynthesis, respiratory electron transport), translation (ribosomal structure and biogenesis) and carbohydrate transport and metabolism. Others proteins were related to amino acid transport and metabolism, cell envelope biogenesis, coenzyme metabolism and signal transduction mechanisms. Results implied that these proteins can perform predictable roles in rendering ASP resistance against low and high temperatures. Subsequently, we determined the transcription level of 38 genes in vivo in response to temperature and identified them by qRT-PCR. We found that the 26 differentially expressed proteins, representing 68.4% of the total target genes, maintained consistency between transcription and translation levels. The remaining 12 genes showed inconsistent protein expression with transcription level and accounted for 31.6% of the total target genes.Wang HuiliZhao XiaokaiLin MeiliRandy A DahlgrenChen WeiZhou JaiopengXu ChengyangJin ChunleiXu YiWang XuedongDing LiBao QiyuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e83485 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wang Huili Zhao Xiaokai Lin Meili Randy A Dahlgren Chen Wei Zhou Jaiopeng Xu Chengyang Jin Chunlei Xu Yi Wang Xuedong Ding Li Bao Qiyu Proteomic analysis and qRT-PCR verification of temperature response to Arthrospira (Spirulina) platensis.
description	Arthrospira (Spirulina) platensis (ASP) is a representative filamentous, non-N2-fixing cyanobacterium that has great potential to enhance the food supply and possesses several valuable physiological features. ASP tolerates high and low temperatures along with highly alkaline and salty environments, and can strongly resist oxidation and irradiation. Based on genomic sequencing of ASP, we compared the protein expression profiles of this organism under different temperature conditions (15°C, 35°Cand 45°C) using 2-DE and peptide mass fingerprinting techniques. A total of 122 proteins having a significant differential expression response to temperature were retrieved. Of the positively expressed proteins, the homologies of 116 ASP proteins were found in Arthrospira (81 proteins in Arthrospira platensis str. Paraca and 35 in Arthrospira maxima CS-328). The other 6 proteins have high homology with other microorganisms. We classified the 122 differentially expressed positive proteins into 14 functions using the COG database, and characterized their respective KEGG metabolism pathways. The results demonstrated that these differentially expressed proteins are mainly involved in post-translational modification (protein turnover, chaperones), energy metabolism (photosynthesis, respiratory electron transport), translation (ribosomal structure and biogenesis) and carbohydrate transport and metabolism. Others proteins were related to amino acid transport and metabolism, cell envelope biogenesis, coenzyme metabolism and signal transduction mechanisms. Results implied that these proteins can perform predictable roles in rendering ASP resistance against low and high temperatures. Subsequently, we determined the transcription level of 38 genes in vivo in response to temperature and identified them by qRT-PCR. We found that the 26 differentially expressed proteins, representing 68.4% of the total target genes, maintained consistency between transcription and translation levels. The remaining 12 genes showed inconsistent protein expression with transcription level and accounted for 31.6% of the total target genes.
format	article
author	Wang Huili Zhao Xiaokai Lin Meili Randy A Dahlgren Chen Wei Zhou Jaiopeng Xu Chengyang Jin Chunlei Xu Yi Wang Xuedong Ding Li Bao Qiyu
author_facet	Wang Huili Zhao Xiaokai Lin Meili Randy A Dahlgren Chen Wei Zhou Jaiopeng Xu Chengyang Jin Chunlei Xu Yi Wang Xuedong Ding Li Bao Qiyu
author_sort	Wang Huili
title	Proteomic analysis and qRT-PCR verification of temperature response to Arthrospira (Spirulina) platensis.
title_short	Proteomic analysis and qRT-PCR verification of temperature response to Arthrospira (Spirulina) platensis.
title_full	Proteomic analysis and qRT-PCR verification of temperature response to Arthrospira (Spirulina) platensis.
title_fullStr	Proteomic analysis and qRT-PCR verification of temperature response to Arthrospira (Spirulina) platensis.
title_full_unstemmed	Proteomic analysis and qRT-PCR verification of temperature response to Arthrospira (Spirulina) platensis.
title_sort	proteomic analysis and qrt-pcr verification of temperature response to arthrospira (spirulina) platensis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e052696018f743458439e81ded94c935
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Proteomic analysis and qRT-PCR verification of temperature response to Arthrospira (Spirulina) platensis.

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