Circulating Tumor Cell Detection in Lung Cancer Animal Model

Background: Metastasis and recurrence of primary cancer are the main causes of cancer mortality. Disseminated tumor cells refer to cancer cells that cause metastasis from primary cancer to other organs. Several recent studies have suggested that circulating tumor cells (CTCs) are associated with the...

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Autores principales:	Yooyoung Chong, Yong Chae Jung, Euidoo Hwang, Hyun Jin Cho, Min-Woong Kang, Myung Hoon Na
Formato:	article
Lenguaje:	EN
Publicado:	Korean Society for Thoracic & Cardiovascular Surgery 2021
Materias:	animal model lung neoplasms malignant tumor Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e053f15b5e0d4cd4a21d4f152252693b
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spelling	oai:doaj.org-article:e053f15b5e0d4cd4a21d4f152252693b2021-12-03T01:19:47ZCirculating Tumor Cell Detection in Lung Cancer Animal Model10.5090/jcs.21.0442765-16062765-1614https://doaj.org/article/e053f15b5e0d4cd4a21d4f152252693b2021-12-01T00:00:00Zhttps://doaj.org/toc/2765-1606https://doaj.org/toc/2765-1614Background: Metastasis and recurrence of primary cancer are the main causes of cancer mortality. Disseminated tumor cells refer to cancer cells that cause metastasis from primary cancer to other organs. Several recent studies have suggested that circulating tumor cells (CTCs) are associated with the clinical stage, cancer recurrence, cancer metastasis, and prognosis. There are several methods of isolating CTCs from whole blood; in particular, using a membrane filtration system is advantageous due to its cost-effectiveness and availability in clinical settings. In this study, an animal model of lung cancer was established in nude mice using the human large cell lung cancer cell line H460. Methods: Six-week-old nude mice were used. The H460 lung cancer cell line was injected subcutaneously into the nude mice. Blood samples were obtained from the orbital area before cell line injection, 2 weeks after injection, and 2 weeks after tumor excision. Blood samples were filtered using a polycarbonate 12-well Transwell membrane (Corning Inc., Corning, NY, USA). An indirect immunofluorescence assay was performed with the epithelial cell adhesion molecule antibody. The number of stained cells was counted using fluorescence microscopy. Results: The average size of the tumor masses was 35.83 mm. The stained cells were counted before inoculation, 2 weeks after inoculation, and 2 weeks after tumor excision. Cancer cells generally increased after inoculation and decreased after tumor resection. Conclusion: The CTC detection method using the commercial polycarbonate 12-well Transwell (Corning Inc.) membrane is advantageous in terms of cost-effectiveness and convenience.Yooyoung ChongYong Chae JungEuidoo HwangHyun Jin ChoMin-Woong KangMyung Hoon NaKorean Society for Thoracic & Cardiovascular Surgeryarticleanimal modellung neoplasmsmalignant tumorMedicine (General)R5-920ENJournal of Chest Surgery, Vol 54, Iss 6, Pp 460-465 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	animal model lung neoplasms malignant tumor Medicine (General) R5-920
spellingShingle	animal model lung neoplasms malignant tumor Medicine (General) R5-920 Yooyoung Chong Yong Chae Jung Euidoo Hwang Hyun Jin Cho Min-Woong Kang Myung Hoon Na Circulating Tumor Cell Detection in Lung Cancer Animal Model
description	Background: Metastasis and recurrence of primary cancer are the main causes of cancer mortality. Disseminated tumor cells refer to cancer cells that cause metastasis from primary cancer to other organs. Several recent studies have suggested that circulating tumor cells (CTCs) are associated with the clinical stage, cancer recurrence, cancer metastasis, and prognosis. There are several methods of isolating CTCs from whole blood; in particular, using a membrane filtration system is advantageous due to its cost-effectiveness and availability in clinical settings. In this study, an animal model of lung cancer was established in nude mice using the human large cell lung cancer cell line H460. Methods: Six-week-old nude mice were used. The H460 lung cancer cell line was injected subcutaneously into the nude mice. Blood samples were obtained from the orbital area before cell line injection, 2 weeks after injection, and 2 weeks after tumor excision. Blood samples were filtered using a polycarbonate 12-well Transwell membrane (Corning Inc., Corning, NY, USA). An indirect immunofluorescence assay was performed with the epithelial cell adhesion molecule antibody. The number of stained cells was counted using fluorescence microscopy. Results: The average size of the tumor masses was 35.83 mm. The stained cells were counted before inoculation, 2 weeks after inoculation, and 2 weeks after tumor excision. Cancer cells generally increased after inoculation and decreased after tumor resection. Conclusion: The CTC detection method using the commercial polycarbonate 12-well Transwell (Corning Inc.) membrane is advantageous in terms of cost-effectiveness and convenience.
format	article
author	Yooyoung Chong Yong Chae Jung Euidoo Hwang Hyun Jin Cho Min-Woong Kang Myung Hoon Na
author_facet	Yooyoung Chong Yong Chae Jung Euidoo Hwang Hyun Jin Cho Min-Woong Kang Myung Hoon Na
author_sort	Yooyoung Chong
title	Circulating Tumor Cell Detection in Lung Cancer Animal Model
title_short	Circulating Tumor Cell Detection in Lung Cancer Animal Model
title_full	Circulating Tumor Cell Detection in Lung Cancer Animal Model
title_fullStr	Circulating Tumor Cell Detection in Lung Cancer Animal Model
title_full_unstemmed	Circulating Tumor Cell Detection in Lung Cancer Animal Model
title_sort	circulating tumor cell detection in lung cancer animal model
publisher	Korean Society for Thoracic & Cardiovascular Surgery
publishDate	2021
url	https://doaj.org/article/e053f15b5e0d4cd4a21d4f152252693b
work_keys_str_mv	AT yooyoungchong circulatingtumorcelldetectioninlungcanceranimalmodel AT yongchaejung circulatingtumorcelldetectioninlungcanceranimalmodel AT euidoohwang circulatingtumorcelldetectioninlungcanceranimalmodel AT hyunjincho circulatingtumorcelldetectioninlungcanceranimalmodel AT minwoongkang circulatingtumorcelldetectioninlungcanceranimalmodel AT myunghoonna circulatingtumorcelldetectioninlungcanceranimalmodel
_version_	1718374019586064384

Circulating Tumor Cell Detection in Lung Cancer Animal Model

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