A gain series method for accurate EMCCD calibration

Abstract Calibration of the gain and digital conversion factor of an EMCCD is necessary for accurate photon counting. We present a new method to quickly calibrate multiple gain settings of an EMCCD camera. Acquiring gain-series calibration data and analyzing the resulting images with the EMCCD noise...

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Autores principales:	Duncan P. Ryan, Megan K. Dunlap, Martin P. Gelfand, James H. Werner, Alan K. Van Orden, Peter M. Goodwin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e055f243014945a487addb17f815ad91
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spelling	oai:doaj.org-article:e055f243014945a487addb17f815ad912021-12-02T18:50:03ZA gain series method for accurate EMCCD calibration10.1038/s41598-021-97759-62045-2322https://doaj.org/article/e055f243014945a487addb17f815ad912021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97759-6https://doaj.org/toc/2045-2322Abstract Calibration of the gain and digital conversion factor of an EMCCD is necessary for accurate photon counting. We present a new method to quickly calibrate multiple gain settings of an EMCCD camera. Acquiring gain-series calibration data and analyzing the resulting images with the EMCCD noise model more accurately estimates the gain response of the camera. Furthermore, we develop a method to compare the results from different calibration approaches. Gain-series calibration outperforms all other methods in this self-consistency test.Duncan P. RyanMegan K. DunlapMartin P. GelfandJames H. WernerAlan K. Van OrdenPeter M. GoodwinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Duncan P. Ryan Megan K. Dunlap Martin P. Gelfand James H. Werner Alan K. Van Orden Peter M. Goodwin A gain series method for accurate EMCCD calibration
description	Abstract Calibration of the gain and digital conversion factor of an EMCCD is necessary for accurate photon counting. We present a new method to quickly calibrate multiple gain settings of an EMCCD camera. Acquiring gain-series calibration data and analyzing the resulting images with the EMCCD noise model more accurately estimates the gain response of the camera. Furthermore, we develop a method to compare the results from different calibration approaches. Gain-series calibration outperforms all other methods in this self-consistency test.
format	article
author	Duncan P. Ryan Megan K. Dunlap Martin P. Gelfand James H. Werner Alan K. Van Orden Peter M. Goodwin
author_facet	Duncan P. Ryan Megan K. Dunlap Martin P. Gelfand James H. Werner Alan K. Van Orden Peter M. Goodwin
author_sort	Duncan P. Ryan
title	A gain series method for accurate EMCCD calibration
title_short	A gain series method for accurate EMCCD calibration
title_full	A gain series method for accurate EMCCD calibration
title_fullStr	A gain series method for accurate EMCCD calibration
title_full_unstemmed	A gain series method for accurate EMCCD calibration
title_sort	gain series method for accurate emccd calibration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e055f243014945a487addb17f815ad91
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A gain series method for accurate EMCCD calibration

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