Emodin-Conjugated PEGylation of Fe3O4 Nanoparticles for FI/MRI Dual-Modal Imaging and Therapy in Pancreatic Cancer

Shuai Ren, Lina Song, Ying Tian, Li Zhu, Kai Guo, Huifeng Zhang, Zhongqiu Wang Department of Radiology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, 210029, People’s Republic of ChinaCorrespondence: Shuai Ren...

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Autores principales:	Ren S, Song L, Tian Y, Zhu L, Guo K, Zhang H, Wang Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	pancreatic cancer emodin magnetic nanoparticles passive targeting Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e05808e5a6334dd281371cd65694f70b
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spelling	oai:doaj.org-article:e05808e5a6334dd281371cd65694f70b2021-12-02T19:32:41ZEmodin-Conjugated PEGylation of Fe3O4 Nanoparticles for FI/MRI Dual-Modal Imaging and Therapy in Pancreatic Cancer1178-2013https://doaj.org/article/e05808e5a6334dd281371cd65694f70b2021-11-01T00:00:00Zhttps://www.dovepress.com/emodin-conjugated-pegylation-of-fe3o4-nanoparticles-for-fimri-dual-mod-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Shuai Ren, Lina Song, Ying Tian, Li Zhu, Kai Guo, Huifeng Zhang, Zhongqiu Wang Department of Radiology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, 210029, People’s Republic of ChinaCorrespondence: Shuai Ren; Zhongqiu WangDepartment of Radiology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, No. 155 Hanzhong Road, Nanjing, Jiangsu Province, 210029, People’s Republic of ChinaEmail sren001@163.com; zhongqiuwang0815@163.comBackground: Pancreatic cancer (PC) remains a difficult tumor to diagnose and treat. It is often diagnosed as advanced by reason of the anatomical structure of the deep retroperitoneal layer of the pancreas, lack of typical symptoms and effective screening methods to detect this malignancy, resulting in a low survival rate. Emodin (EMO) is an economical natural product with effective treatment and few side effects of cancer treatment. Magnetic nanoparticles (MNPs) can achieve multiplexed imaging and targeted therapy by loading a wide range of functional materials such as fluorescent dyes and therapeutic agents.Purpose: The purpose of this study was to design and evaluate a multifunctional theranostic nanoplatform for PC diagnosis and treatment.Methods: In this study, we successfully developed EMO-loaded, Cy7-functionalized, PEG-coated Fe3O4 (Fe3O4-PEG-Cy7-EMO). Characteristics including morphology, hydrodynamic size, zeta potentials, stability, and magnetic properties of Fe3O4-PEG-Cy7-EMO were evaluated. Fluorescence imaging (FI)/magnetic resonance imaging (MRI) and therapeutic treatment were examined in vitro and in vivo.Results: Fe3O4-PEG-Cy7-EMO nanoparticles had a core size of 9.9 ± 1.2 nm, which showed long-time stability and FI/MRI properties. Bio-transmission electron microscopy (bio-TEM) results showed that Fe3O4-PEG-Cy7-EMO nanoparticles were endocytosed into BxPC-3 cells, while few were observed in hTERT-HPNE cells. Prussian blue staining also confirmed that BxPC-3 cells have a stronger phagocytic ability as compared to hTERT-HPNE cells. Additionally, Fe3O4-PEG-Cy7-EMO had a stronger inhibition effect on BxPC-3 cells than Fe3O4-PEG and EMO. The hemolysis experiment proved that Fe3O4-PEG-Cy7-EMO can be used in vivo experiments. In vivo analysis demonstrated that Fe3O4-PEG-Cy7-EMO enabled FI/MRI dual-modal imaging and targeted therapy in pancreatic tumor xenografted mice.Conclusion: Fe3O4-PEG-Cy7-EMO may serve as a potential theranostic nanoplatform for PC.Keywords: pancreatic cancer, emodin, magnetic nanoparticles, passive targetingRen SSong LTian YZhu LGuo KZhang HWang ZDove Medical Pressarticlepancreatic canceremodinmagnetic nanoparticlespassive targetingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 7463-7478 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pancreatic cancer emodin magnetic nanoparticles passive targeting Medicine (General) R5-920
spellingShingle	pancreatic cancer emodin magnetic nanoparticles passive targeting Medicine (General) R5-920 Ren S Song L Tian Y Zhu L Guo K Zhang H Wang Z Emodin-Conjugated PEGylation of Fe3O4 Nanoparticles for FI/MRI Dual-Modal Imaging and Therapy in Pancreatic Cancer
description	Shuai Ren, Lina Song, Ying Tian, Li Zhu, Kai Guo, Huifeng Zhang, Zhongqiu Wang Department of Radiology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, 210029, People’s Republic of ChinaCorrespondence: Shuai Ren; Zhongqiu WangDepartment of Radiology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, No. 155 Hanzhong Road, Nanjing, Jiangsu Province, 210029, People’s Republic of ChinaEmail sren001@163.com; zhongqiuwang0815@163.comBackground: Pancreatic cancer (PC) remains a difficult tumor to diagnose and treat. It is often diagnosed as advanced by reason of the anatomical structure of the deep retroperitoneal layer of the pancreas, lack of typical symptoms and effective screening methods to detect this malignancy, resulting in a low survival rate. Emodin (EMO) is an economical natural product with effective treatment and few side effects of cancer treatment. Magnetic nanoparticles (MNPs) can achieve multiplexed imaging and targeted therapy by loading a wide range of functional materials such as fluorescent dyes and therapeutic agents.Purpose: The purpose of this study was to design and evaluate a multifunctional theranostic nanoplatform for PC diagnosis and treatment.Methods: In this study, we successfully developed EMO-loaded, Cy7-functionalized, PEG-coated Fe3O4 (Fe3O4-PEG-Cy7-EMO). Characteristics including morphology, hydrodynamic size, zeta potentials, stability, and magnetic properties of Fe3O4-PEG-Cy7-EMO were evaluated. Fluorescence imaging (FI)/magnetic resonance imaging (MRI) and therapeutic treatment were examined in vitro and in vivo.Results: Fe3O4-PEG-Cy7-EMO nanoparticles had a core size of 9.9 ± 1.2 nm, which showed long-time stability and FI/MRI properties. Bio-transmission electron microscopy (bio-TEM) results showed that Fe3O4-PEG-Cy7-EMO nanoparticles were endocytosed into BxPC-3 cells, while few were observed in hTERT-HPNE cells. Prussian blue staining also confirmed that BxPC-3 cells have a stronger phagocytic ability as compared to hTERT-HPNE cells. Additionally, Fe3O4-PEG-Cy7-EMO had a stronger inhibition effect on BxPC-3 cells than Fe3O4-PEG and EMO. The hemolysis experiment proved that Fe3O4-PEG-Cy7-EMO can be used in vivo experiments. In vivo analysis demonstrated that Fe3O4-PEG-Cy7-EMO enabled FI/MRI dual-modal imaging and targeted therapy in pancreatic tumor xenografted mice.Conclusion: Fe3O4-PEG-Cy7-EMO may serve as a potential theranostic nanoplatform for PC.Keywords: pancreatic cancer, emodin, magnetic nanoparticles, passive targeting
format	article
author	Ren S Song L Tian Y Zhu L Guo K Zhang H Wang Z
author_facet	Ren S Song L Tian Y Zhu L Guo K Zhang H Wang Z
author_sort	Ren S
title	Emodin-Conjugated PEGylation of Fe3O4 Nanoparticles for FI/MRI Dual-Modal Imaging and Therapy in Pancreatic Cancer
title_short	Emodin-Conjugated PEGylation of Fe3O4 Nanoparticles for FI/MRI Dual-Modal Imaging and Therapy in Pancreatic Cancer
title_full	Emodin-Conjugated PEGylation of Fe3O4 Nanoparticles for FI/MRI Dual-Modal Imaging and Therapy in Pancreatic Cancer
title_fullStr	Emodin-Conjugated PEGylation of Fe3O4 Nanoparticles for FI/MRI Dual-Modal Imaging and Therapy in Pancreatic Cancer
title_full_unstemmed	Emodin-Conjugated PEGylation of Fe3O4 Nanoparticles for FI/MRI Dual-Modal Imaging and Therapy in Pancreatic Cancer
title_sort	emodin-conjugated pegylation of fe3o4 nanoparticles for fi/mri dual-modal imaging and therapy in pancreatic cancer
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/e05808e5a6334dd281371cd65694f70b
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Emodin-Conjugated PEGylation of Fe3O4 Nanoparticles for FI/MRI Dual-Modal Imaging and Therapy in Pancreatic Cancer

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