Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia

Hasmiwati, Rusjdi SR, Nofita E. 2018. Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia. Biodiversitas 19: 31-36. Aedes aegypti is distributed widely in West Sumatra as a primary vector of Dengue hemorrhagic fever, especiall...

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Autores principales:	HASMIWATI HASMIWATI, SELFI RENITA RUSJDI, EKA NOFITA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	aedes aegypti insecticide resistance ace-1gene susceptibility test Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e05b4689683f4b38b1306191ae88bb6a
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spelling	oai:doaj.org-article:e05b4689683f4b38b1306191ae88bb6a2021-11-16T14:01:06ZDetection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia1412-033X2085-472210.13057/biodiv/d190105https://doaj.org/article/e05b4689683f4b38b1306191ae88bb6a2018-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2318https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Hasmiwati, Rusjdi SR, Nofita E. 2018. Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia. Biodiversitas 19: 31-36. Aedes aegypti is distributed widely in West Sumatra as a primary vector of Dengue hemorrhagic fever, especially in Padang City. Synthetic insecticide control is one currently used method to prevent mosquito-borne diseases. The extensive, long-term application of Temephos along with inappropriate dosages, have resulted in the development of resistance in Ae. aegypti populations. Mutation of the Ace-1 gene, encoding an acetyl cholinesterase, is one of the mechanisms that confer resistance to organophosphate (OP). The Temephos resistance status of Ae. aegypti in Padang city has not yet been studied. This study aimed to investigate the resistance status of Ae. aegypti and identify any possible mutation (s) of the Ace-1 gene in Padang city. Ae. aegypti samples were collected in five population in Padang city (Jati (JT), Gunung Pangilun (GP), Lubuk Minturun (LM), Korong Gadang (KG), and Bandar Buat (BB)). The larval susceptibility to Temephos was tested by larval bioassays with Temephos pestanal at 0.02 mg/L dosages. Larval susceptibility was determined by mortality percentage values. The relationship between Ace-1 genotypes and the resistant phenotype was analyzed by percentage of genotype frequency. Out of five populations, assessed by larval bioassays, JT and GP were resistant to Temephos; LM, KG, and BB were tolerant. A total of 50 individuals from larval bioassays were genotyped for Ace-1 gene. Our findings showed that Ace-1 was 495 bp in length. Mutation was not found in the G119S location but in the T506T location. Three alleles in T506T location were detected, including a wild type allele, TT (65.21%), and two mutant alleles, TA (26.08%), AA (8.69%). The use of Temephos showed that some Ae. aegypti populations were resistant, others were tolerant, but no population was vulnerable to Temephos. A novel mutation was detected as substitution in T506T location (ACT>ACA).HASMIWATI HASMIWATISELFI RENITA RUSJDIEKA NOFITAMBI & UNS Soloarticleaedes aegyptiinsecticide resistanceace-1genesusceptibility testBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 1, Pp 31-36 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	aedes aegypti insecticide resistance ace-1gene susceptibility test Biology (General) QH301-705.5
spellingShingle	aedes aegypti insecticide resistance ace-1gene susceptibility test Biology (General) QH301-705.5 HASMIWATI HASMIWATI SELFI RENITA RUSJDI EKA NOFITA Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia
description	Hasmiwati, Rusjdi SR, Nofita E. 2018. Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia. Biodiversitas 19: 31-36. Aedes aegypti is distributed widely in West Sumatra as a primary vector of Dengue hemorrhagic fever, especially in Padang City. Synthetic insecticide control is one currently used method to prevent mosquito-borne diseases. The extensive, long-term application of Temephos along with inappropriate dosages, have resulted in the development of resistance in Ae. aegypti populations. Mutation of the Ace-1 gene, encoding an acetyl cholinesterase, is one of the mechanisms that confer resistance to organophosphate (OP). The Temephos resistance status of Ae. aegypti in Padang city has not yet been studied. This study aimed to investigate the resistance status of Ae. aegypti and identify any possible mutation (s) of the Ace-1 gene in Padang city. Ae. aegypti samples were collected in five population in Padang city (Jati (JT), Gunung Pangilun (GP), Lubuk Minturun (LM), Korong Gadang (KG), and Bandar Buat (BB)). The larval susceptibility to Temephos was tested by larval bioassays with Temephos pestanal at 0.02 mg/L dosages. Larval susceptibility was determined by mortality percentage values. The relationship between Ace-1 genotypes and the resistant phenotype was analyzed by percentage of genotype frequency. Out of five populations, assessed by larval bioassays, JT and GP were resistant to Temephos; LM, KG, and BB were tolerant. A total of 50 individuals from larval bioassays were genotyped for Ace-1 gene. Our findings showed that Ace-1 was 495 bp in length. Mutation was not found in the G119S location but in the T506T location. Three alleles in T506T location were detected, including a wild type allele, TT (65.21%), and two mutant alleles, TA (26.08%), AA (8.69%). The use of Temephos showed that some Ae. aegypti populations were resistant, others were tolerant, but no population was vulnerable to Temephos. A novel mutation was detected as substitution in T506T location (ACT>ACA).
format	article
author	HASMIWATI HASMIWATI SELFI RENITA RUSJDI EKA NOFITA
author_facet	HASMIWATI HASMIWATI SELFI RENITA RUSJDI EKA NOFITA
author_sort	HASMIWATI HASMIWATI
title	Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia
title_short	Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia
title_full	Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia
title_fullStr	Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia
title_full_unstemmed	Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia
title_sort	detection of ace-1 gene with insecticides resistance in aedes aegypti populations from dhf-endemic areas in padang, indonesia
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/e05b4689683f4b38b1306191ae88bb6a
work_keys_str_mv	AT hasmiwatihasmiwati detectionoface1genewithinsecticidesresistanceinaedesaegyptipopulationsfromdhfendemicareasinpadangindonesia AT selfirenitarusjdi detectionoface1genewithinsecticidesresistanceinaedesaegyptipopulationsfromdhfendemicareasinpadangindonesia AT ekanofita detectionoface1genewithinsecticidesresistanceinaedesaegyptipopulationsfromdhfendemicareasinpadangindonesia
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Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia

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