Carboxylesterase activities as potential biomarkers of pollution in marine pelagic predators

Assessment of chemical exposures in the marine environment is frequently undertaken in sedentary organisms inhabiting coastal environments. However, predatory pelagic fish should be considered sentinel species, as they play an important role in the sustainability of the ecosystems due to their high...

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Autores principales:	David Nos, Joan Navarro, David Macías, Montserrat Solé
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biomarkers Carboxylesterases Dichlorvos Marine predators Tuna Trophic level Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/e0619da28bbf4d9b940f28e7648e286b
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spelling	oai:doaj.org-article:e0619da28bbf4d9b940f28e7648e286b2021-12-01T04:39:14ZCarboxylesterase activities as potential biomarkers of pollution in marine pelagic predators1470-160X10.1016/j.ecolind.2020.107217https://doaj.org/article/e0619da28bbf4d9b940f28e7648e286b2021-03-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1470160X20311560https://doaj.org/toc/1470-160XAssessment of chemical exposures in the marine environment is frequently undertaken in sedentary organisms inhabiting coastal environments. However, predatory pelagic fish should be considered sentinel species, as they play an important role in the sustainability of the ecosystems due to their high position in trophic webs. In this study, carboxylesterase (CE) activities were analysed in four predatory tuna species of commercial interest along the western Mediterranean Sea: little tunny (Euthynnus alletteratus), Atlantic bonito (Sarda sarda), bullet tuna (Auxis rochei) and albacore tuna (Thunnus alalunga). CEs are potential biomarkers of chemical exposure, as they are an important family of enzymes involved in the metabolism of xenobiotic and endogenous compounds. CE measures were taken from the liver of these tuna species using five commercial substrates: 4-nitrophenyl acetate (4NPA), 4-nitrophenyl butyrate (4NPB), 1-naphthyl acetate (1NA), 1-naphthyl butyrate (1NB), and 2-naphthyl acetate (2NA). Butyrate substrates (1NB and 4NPB) yielded the highest hydrolysis rates, and were thus the best substrates for these measures. CE activities differed between species. The larger differences were attained with 1NB-CE, with higher activities seen in bullet tuna, followed by little tunny, Atlantic bonito and albacore tuna. Individual size was identified as one of the main factors modulating CE activities, while there was no evidence for a role for trophic level (measured as δ15N). Using little tunny as sentinel, no geographical differences but inter-annual variation in CE activity was observed. The kinetic parameters and in vitro exposure to the pesticide dichlorvos provided complementary information on the sensitivity of tuna CEs to this model pesticide. Our results propose that the little tunny could be considered a potential bioindicator species in the pelagic realm.David NosJoan NavarroDavid MacíasMontserrat SoléElsevierarticleBiomarkersCarboxylesterasesDichlorvosMarine predatorsTunaTrophic levelEcologyQH540-549.5ENEcological Indicators, Vol 122, Iss , Pp 107217- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biomarkers Carboxylesterases Dichlorvos Marine predators Tuna Trophic level Ecology QH540-549.5
spellingShingle	Biomarkers Carboxylesterases Dichlorvos Marine predators Tuna Trophic level Ecology QH540-549.5 David Nos Joan Navarro David Macías Montserrat Solé Carboxylesterase activities as potential biomarkers of pollution in marine pelagic predators
description	Assessment of chemical exposures in the marine environment is frequently undertaken in sedentary organisms inhabiting coastal environments. However, predatory pelagic fish should be considered sentinel species, as they play an important role in the sustainability of the ecosystems due to their high position in trophic webs. In this study, carboxylesterase (CE) activities were analysed in four predatory tuna species of commercial interest along the western Mediterranean Sea: little tunny (Euthynnus alletteratus), Atlantic bonito (Sarda sarda), bullet tuna (Auxis rochei) and albacore tuna (Thunnus alalunga). CEs are potential biomarkers of chemical exposure, as they are an important family of enzymes involved in the metabolism of xenobiotic and endogenous compounds. CE measures were taken from the liver of these tuna species using five commercial substrates: 4-nitrophenyl acetate (4NPA), 4-nitrophenyl butyrate (4NPB), 1-naphthyl acetate (1NA), 1-naphthyl butyrate (1NB), and 2-naphthyl acetate (2NA). Butyrate substrates (1NB and 4NPB) yielded the highest hydrolysis rates, and were thus the best substrates for these measures. CE activities differed between species. The larger differences were attained with 1NB-CE, with higher activities seen in bullet tuna, followed by little tunny, Atlantic bonito and albacore tuna. Individual size was identified as one of the main factors modulating CE activities, while there was no evidence for a role for trophic level (measured as δ15N). Using little tunny as sentinel, no geographical differences but inter-annual variation in CE activity was observed. The kinetic parameters and in vitro exposure to the pesticide dichlorvos provided complementary information on the sensitivity of tuna CEs to this model pesticide. Our results propose that the little tunny could be considered a potential bioindicator species in the pelagic realm.
format	article
author	David Nos Joan Navarro David Macías Montserrat Solé
author_facet	David Nos Joan Navarro David Macías Montserrat Solé
author_sort	David Nos
title	Carboxylesterase activities as potential biomarkers of pollution in marine pelagic predators
title_short	Carboxylesterase activities as potential biomarkers of pollution in marine pelagic predators
title_full	Carboxylesterase activities as potential biomarkers of pollution in marine pelagic predators
title_fullStr	Carboxylesterase activities as potential biomarkers of pollution in marine pelagic predators
title_full_unstemmed	Carboxylesterase activities as potential biomarkers of pollution in marine pelagic predators
title_sort	carboxylesterase activities as potential biomarkers of pollution in marine pelagic predators
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e0619da28bbf4d9b940f28e7648e286b
work_keys_str_mv	AT davidnos carboxylesteraseactivitiesaspotentialbiomarkersofpollutioninmarinepelagicpredators AT joannavarro carboxylesteraseactivitiesaspotentialbiomarkersofpollutioninmarinepelagicpredators AT davidmacias carboxylesteraseactivitiesaspotentialbiomarkersofpollutioninmarinepelagicpredators AT montserratsole carboxylesteraseactivitiesaspotentialbiomarkersofpollutioninmarinepelagicpredators
_version_	1718405874321457152

Carboxylesterase activities as potential biomarkers of pollution in marine pelagic predators

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