Characterization of Staphylococcal Cassette Chromosome <italic toggle="yes">mec</italic> Elements from Methicillin-Resistant <italic toggle="yes">Staphylococcus pseudintermedius</italic> Infections in Australian Animals

ABSTRACT We examined the oxacillin resistance phenotype and genomic structure of staphylococcal cassette chromosome mec (SCCmec) elements from 77 veterinary methicillin-resistant Staphylococcus pseudintermedius (MRSP) isolates. Isolates were characterized by oxacillin broth microdilution, whole-geno...

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Autores principales:	Kate A. Worthing, Sybille Schwendener, Vincent Perreten, Sugiyono Saputra, Geoffrey W. Coombs, Stanley Pang, Mark R. Davies, Sam Abraham, Darren J. Trott, Jacqueline M. Norris
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	SCCmec Staphylococcus pseudintermedius antimicrobial resistance methicillin resistance veterinary zoonotic infections Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e06bebcbd9b84e09855a497931e1d4cd
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spelling	oai:doaj.org-article:e06bebcbd9b84e09855a497931e1d4cd2021-11-15T15:22:22ZCharacterization of Staphylococcal Cassette Chromosome <italic toggle="yes">mec</italic> Elements from Methicillin-Resistant <italic toggle="yes">Staphylococcus pseudintermedius</italic> Infections in Australian Animals10.1128/mSphere.00491-182379-5042https://doaj.org/article/e06bebcbd9b84e09855a497931e1d4cd2018-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00491-18https://doaj.org/toc/2379-5042ABSTRACT We examined the oxacillin resistance phenotype and genomic structure of staphylococcal cassette chromosome mec (SCCmec) elements from 77 veterinary methicillin-resistant Staphylococcus pseudintermedius (MRSP) isolates. Isolates were characterized by oxacillin broth microdilution, whole-genome sequencing, and bioformatics analysis. Five previously described SCCmec elements, and a sixth novel element, were identified: SCCmec III (also known as II-III), ΨSCCmec57395, and SCCmecNA45 (a SCCmec VII variant), all previously described in MRSP, and SCCmec IVg and SCCmec VT, previously described in both methicillin-resistant Staphylococcus aureus (MRSA) and MRSP. The sixth element was novel and found among nine geographically clustered isolates. This novel pseudostaphylococcal cassette chromosome (ΨSCCmecKW21) contained a class A mec gene complex but lacked ccr genes. It also harbored heavy metal (cadmium) resistance determinants. The median oxacillin MIC values among ΨSCCmecKW21, SCCmec III, and SCCmec VT isolates were significantly higher than those determined for the SCCmecNA45 VII variant isolates and ΨSCCmec57395 and SCCmec IVg isolates. ΨSCCmecKW21 was found exclusively in sequence type 497 (ST497), an MRSP clone that is locally successful in Victoria, Australia. Future studies are necessary to determine if this clone has disseminated further afield and if ΨSCCmecKW21 has moved into other MRSP lineages or staphylococcal species. IMPORTANCE Staphylococcus pseudintermedius is a significant veterinary pathogen and occasional cause of infections in humans. β-Lactams are an important group of antimicrobials used to treat staphylococcal infections in humans and animals. However, when staphylococci become methicillin resistant via the acquisition of a mobile genetic element called staphylococcal cassette chromosome mec (SCCmec), they become resistant to all β-lactams. This study detected a novel SCCmec element among a cluster of methicillin-resistant S. pseudintermedius isolates from animals in Australia. It also detected SCCmec elements in S. pseudintermedius that had high similarity to those identified in methicillin-resistant Staphylococcus aureus, demonstrating how human and animal pathogens can share the same resistance determinants.Kate A. WorthingSybille SchwendenerVincent PerretenSugiyono SaputraGeoffrey W. CoombsStanley PangMark R. DaviesSam AbrahamDarren J. TrottJacqueline M. NorrisAmerican Society for MicrobiologyarticleSCCmecStaphylococcus pseudintermediusantimicrobial resistancemethicillin resistanceveterinaryzoonotic infectionsMicrobiologyQR1-502ENmSphere, Vol 3, Iss 6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	SCCmec Staphylococcus pseudintermedius antimicrobial resistance methicillin resistance veterinary zoonotic infections Microbiology QR1-502
spellingShingle	SCCmec Staphylococcus pseudintermedius antimicrobial resistance methicillin resistance veterinary zoonotic infections Microbiology QR1-502 Kate A. Worthing Sybille Schwendener Vincent Perreten Sugiyono Saputra Geoffrey W. Coombs Stanley Pang Mark R. Davies Sam Abraham Darren J. Trott Jacqueline M. Norris Characterization of Staphylococcal Cassette Chromosome <italic toggle="yes">mec</italic> Elements from Methicillin-Resistant <italic toggle="yes">Staphylococcus pseudintermedius</italic> Infections in Australian Animals
description	ABSTRACT We examined the oxacillin resistance phenotype and genomic structure of staphylococcal cassette chromosome mec (SCCmec) elements from 77 veterinary methicillin-resistant Staphylococcus pseudintermedius (MRSP) isolates. Isolates were characterized by oxacillin broth microdilution, whole-genome sequencing, and bioformatics analysis. Five previously described SCCmec elements, and a sixth novel element, were identified: SCCmec III (also known as II-III), ΨSCCmec57395, and SCCmecNA45 (a SCCmec VII variant), all previously described in MRSP, and SCCmec IVg and SCCmec VT, previously described in both methicillin-resistant Staphylococcus aureus (MRSA) and MRSP. The sixth element was novel and found among nine geographically clustered isolates. This novel pseudostaphylococcal cassette chromosome (ΨSCCmecKW21) contained a class A mec gene complex but lacked ccr genes. It also harbored heavy metal (cadmium) resistance determinants. The median oxacillin MIC values among ΨSCCmecKW21, SCCmec III, and SCCmec VT isolates were significantly higher than those determined for the SCCmecNA45 VII variant isolates and ΨSCCmec57395 and SCCmec IVg isolates. ΨSCCmecKW21 was found exclusively in sequence type 497 (ST497), an MRSP clone that is locally successful in Victoria, Australia. Future studies are necessary to determine if this clone has disseminated further afield and if ΨSCCmecKW21 has moved into other MRSP lineages or staphylococcal species. IMPORTANCE Staphylococcus pseudintermedius is a significant veterinary pathogen and occasional cause of infections in humans. β-Lactams are an important group of antimicrobials used to treat staphylococcal infections in humans and animals. However, when staphylococci become methicillin resistant via the acquisition of a mobile genetic element called staphylococcal cassette chromosome mec (SCCmec), they become resistant to all β-lactams. This study detected a novel SCCmec element among a cluster of methicillin-resistant S. pseudintermedius isolates from animals in Australia. It also detected SCCmec elements in S. pseudintermedius that had high similarity to those identified in methicillin-resistant Staphylococcus aureus, demonstrating how human and animal pathogens can share the same resistance determinants.
format	article
author	Kate A. Worthing Sybille Schwendener Vincent Perreten Sugiyono Saputra Geoffrey W. Coombs Stanley Pang Mark R. Davies Sam Abraham Darren J. Trott Jacqueline M. Norris
author_facet	Kate A. Worthing Sybille Schwendener Vincent Perreten Sugiyono Saputra Geoffrey W. Coombs Stanley Pang Mark R. Davies Sam Abraham Darren J. Trott Jacqueline M. Norris
author_sort	Kate A. Worthing
title	Characterization of Staphylococcal Cassette Chromosome <italic toggle="yes">mec</italic> Elements from Methicillin-Resistant <italic toggle="yes">Staphylococcus pseudintermedius</italic> Infections in Australian Animals
title_short	Characterization of Staphylococcal Cassette Chromosome <italic toggle="yes">mec</italic> Elements from Methicillin-Resistant <italic toggle="yes">Staphylococcus pseudintermedius</italic> Infections in Australian Animals
title_full	Characterization of Staphylococcal Cassette Chromosome <italic toggle="yes">mec</italic> Elements from Methicillin-Resistant <italic toggle="yes">Staphylococcus pseudintermedius</italic> Infections in Australian Animals
title_fullStr	Characterization of Staphylococcal Cassette Chromosome <italic toggle="yes">mec</italic> Elements from Methicillin-Resistant <italic toggle="yes">Staphylococcus pseudintermedius</italic> Infections in Australian Animals
title_full_unstemmed	Characterization of Staphylococcal Cassette Chromosome <italic toggle="yes">mec</italic> Elements from Methicillin-Resistant <italic toggle="yes">Staphylococcus pseudintermedius</italic> Infections in Australian Animals
title_sort	characterization of staphylococcal cassette chromosome <italic toggle="yes">mec</italic> elements from methicillin-resistant <italic toggle="yes">staphylococcus pseudintermedius</italic> infections in australian animals
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/e06bebcbd9b84e09855a497931e1d4cd
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Characterization of Staphylococcal Cassette Chromosome <italic toggle="yes">mec</italic> Elements from Methicillin-Resistant <italic toggle="yes">Staphylococcus pseudintermedius</italic> Infections in Australian Animals

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