Confining Trypanosoma brucei in emulsion droplets reveals population variabilities in division rates and improves in vitro cultivation

Abstract Trypanosome parasites are infecting mammals in Sub-Saharan Africa and are transmitted between hosts through bites of the tsetse fly. The transmission from the insect vector to the mammal host causes a number of metabolic and physiological changes. A fraction of the population continuously a...

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Autores principales:	Simone H. Oldenburg, Lionel Buisson, Thomas Beneyton, Deniz Pekin, Magali Thonnus, Frédéric Bringaud, Loïc Rivière, Jean-Christophe Baret
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e0705906537b426d891347e2de812905
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spelling	oai:doaj.org-article:e0705906537b426d891347e2de8129052021-12-02T18:02:15ZConfining Trypanosoma brucei in emulsion droplets reveals population variabilities in division rates and improves in vitro cultivation10.1038/s41598-021-97356-72045-2322https://doaj.org/article/e0705906537b426d891347e2de8129052021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97356-7https://doaj.org/toc/2045-2322Abstract Trypanosome parasites are infecting mammals in Sub-Saharan Africa and are transmitted between hosts through bites of the tsetse fly. The transmission from the insect vector to the mammal host causes a number of metabolic and physiological changes. A fraction of the population continuously adapt to the immune system of the host, indicating heterogeneity at the population level. Yet, the cell to cell variability in populations is mostly unknown. We develop here an analytical method for quantitative measurements at the single cell level based on encapsulation and cultivation of single-cell Trypanosoma brucei in emulsion droplets. We first show that mammalian stage trypanosomes survive for several hours to days in droplets, with an influence of droplet size on both survival and growth. We unravel various growth patterns within a population and find that droplet cultivation of trypanosomes results in 10-fold higher cell densities of the highest dividing cell variants compared to standard cultivation techniques. Some variants reach final cell titers in droplets closer to what is observed in nature than standard culture, of practical interest for cell production. Droplet microfluidics is therefore a promising tool for trypanosome cultivation and analysis with further potential for high-throughput single cell trypanosome analysis.Simone H. OldenburgLionel BuissonThomas BeneytonDeniz PekinMagali ThonnusFrédéric BringaudLoïc RivièreJean-Christophe BaretNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simone H. Oldenburg Lionel Buisson Thomas Beneyton Deniz Pekin Magali Thonnus Frédéric Bringaud Loïc Rivière Jean-Christophe Baret Confining Trypanosoma brucei in emulsion droplets reveals population variabilities in division rates and improves in vitro cultivation
description	Abstract Trypanosome parasites are infecting mammals in Sub-Saharan Africa and are transmitted between hosts through bites of the tsetse fly. The transmission from the insect vector to the mammal host causes a number of metabolic and physiological changes. A fraction of the population continuously adapt to the immune system of the host, indicating heterogeneity at the population level. Yet, the cell to cell variability in populations is mostly unknown. We develop here an analytical method for quantitative measurements at the single cell level based on encapsulation and cultivation of single-cell Trypanosoma brucei in emulsion droplets. We first show that mammalian stage trypanosomes survive for several hours to days in droplets, with an influence of droplet size on both survival and growth. We unravel various growth patterns within a population and find that droplet cultivation of trypanosomes results in 10-fold higher cell densities of the highest dividing cell variants compared to standard cultivation techniques. Some variants reach final cell titers in droplets closer to what is observed in nature than standard culture, of practical interest for cell production. Droplet microfluidics is therefore a promising tool for trypanosome cultivation and analysis with further potential for high-throughput single cell trypanosome analysis.
format	article
author	Simone H. Oldenburg Lionel Buisson Thomas Beneyton Deniz Pekin Magali Thonnus Frédéric Bringaud Loïc Rivière Jean-Christophe Baret
author_facet	Simone H. Oldenburg Lionel Buisson Thomas Beneyton Deniz Pekin Magali Thonnus Frédéric Bringaud Loïc Rivière Jean-Christophe Baret
author_sort	Simone H. Oldenburg
title	Confining Trypanosoma brucei in emulsion droplets reveals population variabilities in division rates and improves in vitro cultivation
title_short	Confining Trypanosoma brucei in emulsion droplets reveals population variabilities in division rates and improves in vitro cultivation
title_full	Confining Trypanosoma brucei in emulsion droplets reveals population variabilities in division rates and improves in vitro cultivation
title_fullStr	Confining Trypanosoma brucei in emulsion droplets reveals population variabilities in division rates and improves in vitro cultivation
title_full_unstemmed	Confining Trypanosoma brucei in emulsion droplets reveals population variabilities in division rates and improves in vitro cultivation
title_sort	confining trypanosoma brucei in emulsion droplets reveals population variabilities in division rates and improves in vitro cultivation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e0705906537b426d891347e2de812905
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Confining Trypanosoma brucei in emulsion droplets reveals population variabilities in division rates and improves in vitro cultivation

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