Improved antigen cross-presentation by polyethyleneimine-based nanoparticles

Jian Chen1, Zhengrong Li1, Hong Huang2, Yanzhu Yang2, Qian Ding2, Junhua Mai1, Wei Guo2, Yuhong Xu21School of Life Sciences and Biotechnology, 2School of Pharmacy, Shanghai Jiao Tong University, Shanghai, People&#39;s Republic of ChinaPurpose: In the development of therapeutic vaccines again...

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Autores principales:	Jian Chen, Zhengrong Li, Hong Huang, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e074868796134a75ae17e6f704102217
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spelling	oai:doaj.org-article:e074868796134a75ae17e6f7041022172021-12-02T02:34:27ZImproved antigen cross-presentation by polyethyleneimine-based nanoparticles1176-91141178-2013https://doaj.org/article/e074868796134a75ae17e6f7041022172011-01-01T00:00:00Zhttp://www.dovepress.com/improved-antigen-cross-presentation-by-polyethyleneimine-based-nanopar-a6003https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jian Chen1, Zhengrong Li1, Hong Huang2, Yanzhu Yang2, Qian Ding2, Junhua Mai1, Wei Guo2, Yuhong Xu21School of Life Sciences and Biotechnology, 2School of Pharmacy, Shanghai Jiao Tong University, Shanghai, People&#39;s Republic of ChinaPurpose: In the development of therapeutic vaccines against cancer, it is important to design strategies for antigen cross-presentation to stimulate cell-mediated immune responses against tumor antigens.Methods: We developed a polyethyleneimine (PEI)-based protein antigen delivery system to promote cross-presentation through the major histocompatibility complex (MHC) I pathway using ovalbumin (OVA) as a model antigen. PEIs formed nanoparticles with OVA by electrostatic interactions, as demonstrated by electrophoresis analysis, scanning electron microscopy, and photon correlation spectroscopy analysis.Results: The nanoparticles were used to stimulate mouse bone marrow-derived dendritic cells in vitro and resulted in significantly more OVA257&ndash;264/MHC I complex presentation on dendritic cell surfaces. The activated dendritic cells interacted specifically with RF33.70 to stimulate interleukin-2 secretion. The cross-presentation promoting effect was more prominent in dendritic cells that had been cultured for longer periods of time (13 days). Further studies comparing the antigen presentation efficacies by other polyanionic agents, such as PLL or lysosomotropic agents, suggested that the unique &ldquo;proton sponge effect&rdquo; of PEI facilitated antigen escape from the endosome toward the MHC I pathway.Conclusion: Such a PEI-based nanoparticle system may have the potential to be developed into an effective therapeutic vaccine delivery system.Keywords: cross-presentation, polyethyleneimine, dendritic cells, vaccine Jian ChenZhengrong LiHong Huanget alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 77-84 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Jian Chen Zhengrong Li Hong Huang et al Improved antigen cross-presentation by polyethyleneimine-based nanoparticles
description	Jian Chen1, Zhengrong Li1, Hong Huang2, Yanzhu Yang2, Qian Ding2, Junhua Mai1, Wei Guo2, Yuhong Xu21School of Life Sciences and Biotechnology, 2School of Pharmacy, Shanghai Jiao Tong University, Shanghai, People&#39;s Republic of ChinaPurpose: In the development of therapeutic vaccines against cancer, it is important to design strategies for antigen cross-presentation to stimulate cell-mediated immune responses against tumor antigens.Methods: We developed a polyethyleneimine (PEI)-based protein antigen delivery system to promote cross-presentation through the major histocompatibility complex (MHC) I pathway using ovalbumin (OVA) as a model antigen. PEIs formed nanoparticles with OVA by electrostatic interactions, as demonstrated by electrophoresis analysis, scanning electron microscopy, and photon correlation spectroscopy analysis.Results: The nanoparticles were used to stimulate mouse bone marrow-derived dendritic cells in vitro and resulted in significantly more OVA257&ndash;264/MHC I complex presentation on dendritic cell surfaces. The activated dendritic cells interacted specifically with RF33.70 to stimulate interleukin-2 secretion. The cross-presentation promoting effect was more prominent in dendritic cells that had been cultured for longer periods of time (13 days). Further studies comparing the antigen presentation efficacies by other polyanionic agents, such as PLL or lysosomotropic agents, suggested that the unique &ldquo;proton sponge effect&rdquo; of PEI facilitated antigen escape from the endosome toward the MHC I pathway.Conclusion: Such a PEI-based nanoparticle system may have the potential to be developed into an effective therapeutic vaccine delivery system.Keywords: cross-presentation, polyethyleneimine, dendritic cells, vaccine
format	article
author	Jian Chen Zhengrong Li Hong Huang et al
author_facet	Jian Chen Zhengrong Li Hong Huang et al
author_sort	Jian Chen
title	Improved antigen cross-presentation by polyethyleneimine-based nanoparticles
title_short	Improved antigen cross-presentation by polyethyleneimine-based nanoparticles
title_full	Improved antigen cross-presentation by polyethyleneimine-based nanoparticles
title_fullStr	Improved antigen cross-presentation by polyethyleneimine-based nanoparticles
title_full_unstemmed	Improved antigen cross-presentation by polyethyleneimine-based nanoparticles
title_sort	improved antigen cross-presentation by polyethyleneimine-based nanoparticles
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/e074868796134a75ae17e6f704102217
work_keys_str_mv	AT jianchen improvedantigencrosspresentationbypolyethyleneiminebasednanoparticles AT zhengrongli improvedantigencrosspresentationbypolyethyleneiminebasednanoparticles AT honghuang improvedantigencrosspresentationbypolyethyleneiminebasednanoparticles AT etal improvedantigencrosspresentationbypolyethyleneiminebasednanoparticles
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Improved antigen cross-presentation by polyethyleneimine-based nanoparticles

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