Functional characteristics of a novel odorant binding protein in the legume pod borer, Maruca vitrata

Abstract Insect olfaction system plays a key role in the foraging food, pollination, mating, oviposition, reproduction and other insect physiological behavior. Odorant binding protein are widely found in the various olfactory sensilla of different insect antennae and involved in chemical signals dis...

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Autores principales:	Hui Ai, Yuying Liu, Guangyan Long, Yuan Yuan, Shaopei Huang, Yan Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e075543ae0014371b1e5b2323efcb224
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spelling	oai:doaj.org-article:e075543ae0014371b1e5b2323efcb2242021-12-02T18:34:20ZFunctional characteristics of a novel odorant binding protein in the legume pod borer, Maruca vitrata10.1038/s41598-021-93382-72045-2322https://doaj.org/article/e075543ae0014371b1e5b2323efcb2242021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93382-7https://doaj.org/toc/2045-2322Abstract Insect olfaction system plays a key role in the foraging food, pollination, mating, oviposition, reproduction and other insect physiological behavior. Odorant binding protein are widely found in the various olfactory sensilla of different insect antennae and involved in chemical signals discrimination from natural environment. In this study, a novel OBP gene, MvitOBP3 is identified from the legume pod borer, Maruca vitrata, which it mainly harms important legume vegetables including cowpea, soybean and lablab bean. Real-time PCR results demonstrated that MvitOBP3 gene was abundantly expressed in the antennal tissue of M. vitrata, while low levels were distributed in the head, thorax, abdomen, leg and wing of adult moths. The recombinant OBP3 protein was purified using the prokaryotic expression and affinity chromatography system. Fluorescence competitive binding experiments indicated that that MvitOBP3 protein exhibited greater binding affinities with host-plant flower volatiles including Butanoic acid butyl ester, Limonene, 1H-indol-4-ol and 2-methyl-3-phenylpropanal, highlighting they may have attractant activities for the oviposition of female moths on the legume vegetables. Moreover, protein homology modeling and molecular docking analysis revealed that there are six amino acid sites of MvitOBP3 involved in the binding of the host-plant volatiles. These findings will further promote to understand the key role of odorant binding protein during host perception and oviposition of M. vitrata moths, which improve the efficiency of semiochemical-based prevention and monitoring for this pest in the legume vegetables field.Hui AiYuying LiuGuangyan LongYuan YuanShaopei HuangYan ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hui Ai Yuying Liu Guangyan Long Yuan Yuan Shaopei Huang Yan Chen Functional characteristics of a novel odorant binding protein in the legume pod borer, Maruca vitrata
description	Abstract Insect olfaction system plays a key role in the foraging food, pollination, mating, oviposition, reproduction and other insect physiological behavior. Odorant binding protein are widely found in the various olfactory sensilla of different insect antennae and involved in chemical signals discrimination from natural environment. In this study, a novel OBP gene, MvitOBP3 is identified from the legume pod borer, Maruca vitrata, which it mainly harms important legume vegetables including cowpea, soybean and lablab bean. Real-time PCR results demonstrated that MvitOBP3 gene was abundantly expressed in the antennal tissue of M. vitrata, while low levels were distributed in the head, thorax, abdomen, leg and wing of adult moths. The recombinant OBP3 protein was purified using the prokaryotic expression and affinity chromatography system. Fluorescence competitive binding experiments indicated that that MvitOBP3 protein exhibited greater binding affinities with host-plant flower volatiles including Butanoic acid butyl ester, Limonene, 1H-indol-4-ol and 2-methyl-3-phenylpropanal, highlighting they may have attractant activities for the oviposition of female moths on the legume vegetables. Moreover, protein homology modeling and molecular docking analysis revealed that there are six amino acid sites of MvitOBP3 involved in the binding of the host-plant volatiles. These findings will further promote to understand the key role of odorant binding protein during host perception and oviposition of M. vitrata moths, which improve the efficiency of semiochemical-based prevention and monitoring for this pest in the legume vegetables field.
format	article
author	Hui Ai Yuying Liu Guangyan Long Yuan Yuan Shaopei Huang Yan Chen
author_facet	Hui Ai Yuying Liu Guangyan Long Yuan Yuan Shaopei Huang Yan Chen
author_sort	Hui Ai
title	Functional characteristics of a novel odorant binding protein in the legume pod borer, Maruca vitrata
title_short	Functional characteristics of a novel odorant binding protein in the legume pod borer, Maruca vitrata
title_full	Functional characteristics of a novel odorant binding protein in the legume pod borer, Maruca vitrata
title_fullStr	Functional characteristics of a novel odorant binding protein in the legume pod borer, Maruca vitrata
title_full_unstemmed	Functional characteristics of a novel odorant binding protein in the legume pod borer, Maruca vitrata
title_sort	functional characteristics of a novel odorant binding protein in the legume pod borer, maruca vitrata
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e075543ae0014371b1e5b2323efcb224
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Functional characteristics of a novel odorant binding protein in the legume pod borer, Maruca vitrata

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