DMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes

Abstract As an environmental pollutant and carcinogen, 7,12-dimethylbenz[a]anthracene (DMBA) can destroy ovarian follicles at all developmental stages in rodents. However, the underlying molecular mechanism remains obscure. In the present study, we aim to address how DMBA affects the in vitro matura...

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Autores principales:	Zhi-Qiang Song, Xuan Li, Yan-Kui Wang, Zhi-Qiang Du, Cai-Xia Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e075581e2ba64f75ae996da3c9e8ba2a
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spelling	oai:doaj.org-article:e075581e2ba64f75ae996da3c9e8ba2a2021-12-02T16:07:00ZDMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes10.1038/s41598-017-01870-62045-2322https://doaj.org/article/e075581e2ba64f75ae996da3c9e8ba2a2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01870-6https://doaj.org/toc/2045-2322Abstract As an environmental pollutant and carcinogen, 7,12-dimethylbenz[a]anthracene (DMBA) can destroy ovarian follicles at all developmental stages in rodents. However, the underlying molecular mechanism remains obscure. In the present study, we aim to address how DMBA affects the in vitro maturation and development of porcine oocytes. We discovered that for 20 μM DMBA-treated cumulus-oocyte complexes (COCs), the rate of oocyte germinal vesicle breakdown (GVBD) was significantly altered, and the extrusion rate of first polar body was increased. Moreover, oocytes from 20 μM DMBA-treated COCs had significant down-regulation of H3K9me3 and H3K27me3, up-regulation of H3K36me3, higher incidence of DNA double strand breaks (DSBs) and early apoptosis. In striking contrast, none of these changes happened to 20 μM DMBA-treated cumulus-denuded oocytes (CDOs). Furthermore, 20 μM DMBA treatment increased the reactive oxygen species (ROS) level, decreased mitochondrial membrane potential (Δ Ψm), and inhibited developmental competence for oocytes from both COC and CDO groups. Collectively, our data indicate DMBA could act on cumulus cells via the gap junction to disturb the synchronization of nuclear and ooplasmic maturation, and reduce the developmental competence of oocytes.Zhi-Qiang SongXuan LiYan-Kui WangZhi-Qiang DuCai-Xia YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhi-Qiang Song Xuan Li Yan-Kui Wang Zhi-Qiang Du Cai-Xia Yang DMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes
description	Abstract As an environmental pollutant and carcinogen, 7,12-dimethylbenz[a]anthracene (DMBA) can destroy ovarian follicles at all developmental stages in rodents. However, the underlying molecular mechanism remains obscure. In the present study, we aim to address how DMBA affects the in vitro maturation and development of porcine oocytes. We discovered that for 20 μM DMBA-treated cumulus-oocyte complexes (COCs), the rate of oocyte germinal vesicle breakdown (GVBD) was significantly altered, and the extrusion rate of first polar body was increased. Moreover, oocytes from 20 μM DMBA-treated COCs had significant down-regulation of H3K9me3 and H3K27me3, up-regulation of H3K36me3, higher incidence of DNA double strand breaks (DSBs) and early apoptosis. In striking contrast, none of these changes happened to 20 μM DMBA-treated cumulus-denuded oocytes (CDOs). Furthermore, 20 μM DMBA treatment increased the reactive oxygen species (ROS) level, decreased mitochondrial membrane potential (Δ Ψm), and inhibited developmental competence for oocytes from both COC and CDO groups. Collectively, our data indicate DMBA could act on cumulus cells via the gap junction to disturb the synchronization of nuclear and ooplasmic maturation, and reduce the developmental competence of oocytes.
format	article
author	Zhi-Qiang Song Xuan Li Yan-Kui Wang Zhi-Qiang Du Cai-Xia Yang
author_facet	Zhi-Qiang Song Xuan Li Yan-Kui Wang Zhi-Qiang Du Cai-Xia Yang
author_sort	Zhi-Qiang Song
title	DMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes
title_short	DMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes
title_full	DMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes
title_fullStr	DMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes
title_full_unstemmed	DMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes
title_sort	dmba acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e075581e2ba64f75ae996da3c9e8ba2a
work_keys_str_mv	AT zhiqiangsong dmbaactsoncumuluscellstodesynchronizenuclearandcytoplasmicmaturationofpigoocytes AT xuanli dmbaactsoncumuluscellstodesynchronizenuclearandcytoplasmicmaturationofpigoocytes AT yankuiwang dmbaactsoncumuluscellstodesynchronizenuclearandcytoplasmicmaturationofpigoocytes AT zhiqiangdu dmbaactsoncumuluscellstodesynchronizenuclearandcytoplasmicmaturationofpigoocytes AT caixiayang dmbaactsoncumuluscellstodesynchronizenuclearandcytoplasmicmaturationofpigoocytes
_version_	1718384811803934720

DMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes

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