Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina.

<h4>Objective</h4>To undertake a comprehensive analysis of the biochemical tissue composition and passive biomechanical properties of ovine vagina and relate this to the histo-architecture at different reproductive stages as part of the establishment of a large preclinical animal model f...

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Autores principales:	Daniela Ulrich, Sharon L Edwards, Kai Su, Jacinta F White, John A M Ramshaw, Graham Jenkin, Jan Deprest, Anna Rosamilia, Jerome A Werkmeister, Caroline E Gargett
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e08138f39ad74a979a080dff3ec03fd9
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spelling	oai:doaj.org-article:e08138f39ad74a979a080dff3ec03fd92021-11-18T08:24:41ZInfluence of reproductive status on tissue composition and biomechanical properties of ovine vagina.1932-620310.1371/journal.pone.0093172https://doaj.org/article/e08138f39ad74a979a080dff3ec03fd92014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24709913/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>To undertake a comprehensive analysis of the biochemical tissue composition and passive biomechanical properties of ovine vagina and relate this to the histo-architecture at different reproductive stages as part of the establishment of a large preclinical animal model for evaluating regenerative medicine approaches for surgical treatment of pelvic organ prolapse.<h4>Methods</h4>Vaginal tissue was collected from virgin (n = 3), parous (n = 6) and pregnant sheep (n = 6; mean gestation; 132 d; term = 145 d). Tissue histology was analyzed using H+E and Masson's Trichrome staining. Biochemical analysis of the extracellular matrix proteins used a hydroxyproline assay to quantify total collagen, SDS PAGE to measure collagen III/I+III ratios, dimethylmethylene blue to quantify glycosaminoglycans and amino acid analysis to quantify elastin. Uniaxial tensiometry was used to determine the Young's modulus, maximum stress and strain, and permanent strain following cyclic loading.<h4>Results</h4>Vaginal tissue of virgin sheep had the lowest total collagen content and permanent strain. Parous tissue had the highest total collagen and lowest elastin content with concomitant high maximum stress. In contrast, pregnant sheep had the highest elastin and lowest collagen contents, and thickest smooth muscle layer, which was associated with low maximum stress and poor dimensional recovery following repetitive loading.<h4>Conclusion</h4>Pregnant ovine vagina was the most extensible, but the weakest tissue, whereas parous and virgin tissues were strong and elastic. Pregnancy had the greatest impact on tissue composition and biomechanical properties, compatible with significant tissue remodeling as demonstrated in other species. Biochemical changes in tissue protein composition coincide with these altered biomechanical properties.Daniela UlrichSharon L EdwardsKai SuJacinta F WhiteJohn A M RamshawGraham JenkinJan DeprestAnna RosamiliaJerome A WerkmeisterCaroline E GargettPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e93172 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniela Ulrich Sharon L Edwards Kai Su Jacinta F White John A M Ramshaw Graham Jenkin Jan Deprest Anna Rosamilia Jerome A Werkmeister Caroline E Gargett Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina.
description	<h4>Objective</h4>To undertake a comprehensive analysis of the biochemical tissue composition and passive biomechanical properties of ovine vagina and relate this to the histo-architecture at different reproductive stages as part of the establishment of a large preclinical animal model for evaluating regenerative medicine approaches for surgical treatment of pelvic organ prolapse.<h4>Methods</h4>Vaginal tissue was collected from virgin (n = 3), parous (n = 6) and pregnant sheep (n = 6; mean gestation; 132 d; term = 145 d). Tissue histology was analyzed using H+E and Masson's Trichrome staining. Biochemical analysis of the extracellular matrix proteins used a hydroxyproline assay to quantify total collagen, SDS PAGE to measure collagen III/I+III ratios, dimethylmethylene blue to quantify glycosaminoglycans and amino acid analysis to quantify elastin. Uniaxial tensiometry was used to determine the Young's modulus, maximum stress and strain, and permanent strain following cyclic loading.<h4>Results</h4>Vaginal tissue of virgin sheep had the lowest total collagen content and permanent strain. Parous tissue had the highest total collagen and lowest elastin content with concomitant high maximum stress. In contrast, pregnant sheep had the highest elastin and lowest collagen contents, and thickest smooth muscle layer, which was associated with low maximum stress and poor dimensional recovery following repetitive loading.<h4>Conclusion</h4>Pregnant ovine vagina was the most extensible, but the weakest tissue, whereas parous and virgin tissues were strong and elastic. Pregnancy had the greatest impact on tissue composition and biomechanical properties, compatible with significant tissue remodeling as demonstrated in other species. Biochemical changes in tissue protein composition coincide with these altered biomechanical properties.
format	article
author	Daniela Ulrich Sharon L Edwards Kai Su Jacinta F White John A M Ramshaw Graham Jenkin Jan Deprest Anna Rosamilia Jerome A Werkmeister Caroline E Gargett
author_facet	Daniela Ulrich Sharon L Edwards Kai Su Jacinta F White John A M Ramshaw Graham Jenkin Jan Deprest Anna Rosamilia Jerome A Werkmeister Caroline E Gargett
author_sort	Daniela Ulrich
title	Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina.
title_short	Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina.
title_full	Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina.
title_fullStr	Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina.
title_full_unstemmed	Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina.
title_sort	influence of reproductive status on tissue composition and biomechanical properties of ovine vagina.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/e08138f39ad74a979a080dff3ec03fd9
work_keys_str_mv	AT danielaulrich influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT sharonledwards influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT kaisu influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT jacintafwhite influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT johnamramshaw influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT grahamjenkin influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT jandeprest influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT annarosamilia influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT jeromeawerkmeister influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT carolineegargett influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina
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Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina.

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