Stable Reference Gene Selection for qRT-PCR Normalization in Strawberry (<i>Fragaria × ananassa</i>) Leaves under Different Stress and Light-Quality Conditions

Selecting an appropriate reference gene is of crucial importance for improving the accuracy of qRT-PCR analyses. In this study, strawberry (<i>Fragaria ananassa</i>) seedlings were subjected to different environmental conditions including heat, cold, drought, salt, white-light, blue-ligh...

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Autores principales:	Yuntian Ye, Yang Lu, Guangyi Wang, Yongqiang Liu, Yunting Zhang, Haoru Tang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	strawberry reference genes qRT-PCR analysis expression stability Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/e0847a8d5fc34697b2a3cad25da9f119
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spelling	oai:doaj.org-article:e0847a8d5fc34697b2a3cad25da9f1192021-11-25T17:47:18ZStable Reference Gene Selection for qRT-PCR Normalization in Strawberry (<i>Fragaria × ananassa</i>) Leaves under Different Stress and Light-Quality Conditions10.3390/horticulturae71104522311-7524https://doaj.org/article/e0847a8d5fc34697b2a3cad25da9f1192021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/452https://doaj.org/toc/2311-7524Selecting an appropriate reference gene is of crucial importance for improving the accuracy of qRT-PCR analyses. In this study, strawberry (<i>Fragaria ananassa</i>) seedlings were subjected to different environmental conditions including heat, cold, drought, salt, white-light, blue-light, and red-light treatments. The expression levels of seven candidate reference genes, including <i>Fa18S</i>, <i>FaGAPDH</i>, <i>FaPIRUV</i>, <i>FaDBP</i>, <i>FaHISTH4</i>, <i>FaACTIN1</i>, and <i>FaACTIN2</i>, in the strawberry leaves were measured by qRT-PCR. Then, four programs (geNorm, NormFinder, BestKeeper, and RefFinder) were employed as tools to evaluate the expression stability of the candidate reference genes. The results showed that the expression stability of the reference genes varied under different conditions. For the cold stress and white-light treatments, <i>FaACTIN2</i> was evaluated to be the most stable reference gene. <i>FaGAPDH</i> should be used as the reference gene under salt-stress condition and red-light treatment. For the data normalization under drought-stress treatment, <i>FaDBP</i> is the recommended reference gene with the highest expression stability. <i>FaHISTH4</i> was observed to be the best reference gene for data normalization under heat stress and blue-light treatment. This work provides information on selecting reference genes for accurate gene expression analyses of target genes in strawberry leaves under various abiotic stress and light-quality conditions.Yuntian YeYang LuGuangyi WangYongqiang LiuYunting ZhangHaoru TangMDPI AGarticlestrawberryreference genesqRT-PCR analysisexpression stabilityPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 452, p 452 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	strawberry reference genes qRT-PCR analysis expression stability Plant culture SB1-1110
spellingShingle	strawberry reference genes qRT-PCR analysis expression stability Plant culture SB1-1110 Yuntian Ye Yang Lu Guangyi Wang Yongqiang Liu Yunting Zhang Haoru Tang Stable Reference Gene Selection for qRT-PCR Normalization in Strawberry (<i>Fragaria × ananassa</i>) Leaves under Different Stress and Light-Quality Conditions
description	Selecting an appropriate reference gene is of crucial importance for improving the accuracy of qRT-PCR analyses. In this study, strawberry (<i>Fragaria ananassa</i>) seedlings were subjected to different environmental conditions including heat, cold, drought, salt, white-light, blue-light, and red-light treatments. The expression levels of seven candidate reference genes, including <i>Fa18S</i>, <i>FaGAPDH</i>, <i>FaPIRUV</i>, <i>FaDBP</i>, <i>FaHISTH4</i>, <i>FaACTIN1</i>, and <i>FaACTIN2</i>, in the strawberry leaves were measured by qRT-PCR. Then, four programs (geNorm, NormFinder, BestKeeper, and RefFinder) were employed as tools to evaluate the expression stability of the candidate reference genes. The results showed that the expression stability of the reference genes varied under different conditions. For the cold stress and white-light treatments, <i>FaACTIN2</i> was evaluated to be the most stable reference gene. <i>FaGAPDH</i> should be used as the reference gene under salt-stress condition and red-light treatment. For the data normalization under drought-stress treatment, <i>FaDBP</i> is the recommended reference gene with the highest expression stability. <i>FaHISTH4</i> was observed to be the best reference gene for data normalization under heat stress and blue-light treatment. This work provides information on selecting reference genes for accurate gene expression analyses of target genes in strawberry leaves under various abiotic stress and light-quality conditions.
format	article
author	Yuntian Ye Yang Lu Guangyi Wang Yongqiang Liu Yunting Zhang Haoru Tang
author_facet	Yuntian Ye Yang Lu Guangyi Wang Yongqiang Liu Yunting Zhang Haoru Tang
author_sort	Yuntian Ye
title	Stable Reference Gene Selection for qRT-PCR Normalization in Strawberry (<i>Fragaria × ananassa</i>) Leaves under Different Stress and Light-Quality Conditions
title_short	Stable Reference Gene Selection for qRT-PCR Normalization in Strawberry (<i>Fragaria × ananassa</i>) Leaves under Different Stress and Light-Quality Conditions
title_full	Stable Reference Gene Selection for qRT-PCR Normalization in Strawberry (<i>Fragaria × ananassa</i>) Leaves under Different Stress and Light-Quality Conditions
title_fullStr	Stable Reference Gene Selection for qRT-PCR Normalization in Strawberry (<i>Fragaria × ananassa</i>) Leaves under Different Stress and Light-Quality Conditions
title_full_unstemmed	Stable Reference Gene Selection for qRT-PCR Normalization in Strawberry (<i>Fragaria × ananassa</i>) Leaves under Different Stress and Light-Quality Conditions
title_sort	stable reference gene selection for qrt-pcr normalization in strawberry (<i>fragaria × ananassa</i>) leaves under different stress and light-quality conditions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e0847a8d5fc34697b2a3cad25da9f119
work_keys_str_mv	AT yuntianye stablereferencegeneselectionforqrtpcrnormalizationinstrawberryifragariaananassaileavesunderdifferentstressandlightqualityconditions AT yanglu stablereferencegeneselectionforqrtpcrnormalizationinstrawberryifragariaananassaileavesunderdifferentstressandlightqualityconditions AT guangyiwang stablereferencegeneselectionforqrtpcrnormalizationinstrawberryifragariaananassaileavesunderdifferentstressandlightqualityconditions AT yongqiangliu stablereferencegeneselectionforqrtpcrnormalizationinstrawberryifragariaananassaileavesunderdifferentstressandlightqualityconditions AT yuntingzhang stablereferencegeneselectionforqrtpcrnormalizationinstrawberryifragariaananassaileavesunderdifferentstressandlightqualityconditions AT haorutang stablereferencegeneselectionforqrtpcrnormalizationinstrawberryifragariaananassaileavesunderdifferentstressandlightqualityconditions
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Stable Reference Gene Selection for qRT-PCR Normalization in Strawberry (<i>Fragaria × ananassa</i>) Leaves under Different Stress and Light-Quality Conditions

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