Altered inflammatory response in FMRP-deficient microglia

Summary: Fragile X syndrome (FXS) is an inherited intellectual disability with a high risk for comorbid autism spectrum disorders. Since FXS is a genetic disease, patients are more susceptible to environmental factors aggravating symptomatology. However, this confounding interaction between FXS envi...

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Autores principales:	Jennifer M. Parrott, Thomas Oster, Hye Young Lee
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Molecular biology Neuroscience Immunology Science Q
Acceso en línea:	https://doaj.org/article/e088359eab28482abe400d2c0c18a0df
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spelling	oai:doaj.org-article:e088359eab28482abe400d2c0c18a0df2021-11-20T05:09:32ZAltered inflammatory response in FMRP-deficient microglia2589-004210.1016/j.isci.2021.103293https://doaj.org/article/e088359eab28482abe400d2c0c18a0df2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221012621https://doaj.org/toc/2589-0042Summary: Fragile X syndrome (FXS) is an inherited intellectual disability with a high risk for comorbid autism spectrum disorders. Since FXS is a genetic disease, patients are more susceptible to environmental factors aggravating symptomatology. However, this confounding interaction between FXS environmental and genetic risk factors is under-investigated. Here, Fmr1 knock-out (KO) mice and the immune stimulus lipopolysaccharide (LPS) were used to explore this interaction between FXS development and inflammation in microglia, the brain’s primary immune cell. Our results demonstrate that Fmr1 KO and wild-type (WT) microglia are not different in inflammatory outcomes without LPS. However, Fmr1 KO microglia produces an elevated pro-inflammatory and phagocytic response following LPS treatment when compared to WT microglia. Our experiments also revealed baseline differences in mitochondrial function and morphology between WT and Fmr1 KO microglia, which LPS treatment exaggerated. Our data suggest an altered inflammatory mechanism in Fmr1 KO microglia implicating a gene and environment interaction.Jennifer M. ParrottThomas OsterHye Young LeeElsevierarticleMolecular biologyNeuroscienceImmunologyScienceQENiScience, Vol 24, Iss 11, Pp 103293- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Molecular biology Neuroscience Immunology Science Q
spellingShingle	Molecular biology Neuroscience Immunology Science Q Jennifer M. Parrott Thomas Oster Hye Young Lee Altered inflammatory response in FMRP-deficient microglia
description	Summary: Fragile X syndrome (FXS) is an inherited intellectual disability with a high risk for comorbid autism spectrum disorders. Since FXS is a genetic disease, patients are more susceptible to environmental factors aggravating symptomatology. However, this confounding interaction between FXS environmental and genetic risk factors is under-investigated. Here, Fmr1 knock-out (KO) mice and the immune stimulus lipopolysaccharide (LPS) were used to explore this interaction between FXS development and inflammation in microglia, the brain’s primary immune cell. Our results demonstrate that Fmr1 KO and wild-type (WT) microglia are not different in inflammatory outcomes without LPS. However, Fmr1 KO microglia produces an elevated pro-inflammatory and phagocytic response following LPS treatment when compared to WT microglia. Our experiments also revealed baseline differences in mitochondrial function and morphology between WT and Fmr1 KO microglia, which LPS treatment exaggerated. Our data suggest an altered inflammatory mechanism in Fmr1 KO microglia implicating a gene and environment interaction.
format	article
author	Jennifer M. Parrott Thomas Oster Hye Young Lee
author_facet	Jennifer M. Parrott Thomas Oster Hye Young Lee
author_sort	Jennifer M. Parrott
title	Altered inflammatory response in FMRP-deficient microglia
title_short	Altered inflammatory response in FMRP-deficient microglia
title_full	Altered inflammatory response in FMRP-deficient microglia
title_fullStr	Altered inflammatory response in FMRP-deficient microglia
title_full_unstemmed	Altered inflammatory response in FMRP-deficient microglia
title_sort	altered inflammatory response in fmrp-deficient microglia
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e088359eab28482abe400d2c0c18a0df
work_keys_str_mv	AT jennifermparrott alteredinflammatoryresponseinfmrpdeficientmicroglia AT thomasoster alteredinflammatoryresponseinfmrpdeficientmicroglia AT hyeyounglee alteredinflammatoryresponseinfmrpdeficientmicroglia
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Altered inflammatory response in FMRP-deficient microglia

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