Iron overload in endometriosis peritoneal fluid induces early embryo ferroptosis mediated by HMOX1

Abstract Endometriosis is one of the most common disorders that causes infertility in women. Iron is overloaded in endometriosis peritoneal fluid (PF), with harmful effects on early embryo development. However, the mechanism by which endometriosis peritoneal fluid affects embryonic development remai...

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Autores principales:	Shishi Li, Yier Zhou, Qiongxiao Huang, Xiaohua Fu, Ling Zhang, Fang Gao, Zhen Jin, Limei Wu, Chongyi Shu, Xirong Zhang, Weihai Xu, Jing Shu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Publishing Group 2021
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/e08c5e22276946269adac69ff1266fa7
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spelling	oai:doaj.org-article:e08c5e22276946269adac69ff1266fa72021-11-21T12:08:48ZIron overload in endometriosis peritoneal fluid induces early embryo ferroptosis mediated by HMOX110.1038/s41420-021-00751-22058-7716https://doaj.org/article/e08c5e22276946269adac69ff1266fa72021-11-01T00:00:00Zhttps://doi.org/10.1038/s41420-021-00751-2https://doaj.org/toc/2058-7716Abstract Endometriosis is one of the most common disorders that causes infertility in women. Iron is overloaded in endometriosis peritoneal fluid (PF), with harmful effects on early embryo development. However, the mechanism by which endometriosis peritoneal fluid affects embryonic development remains unclear. Hence, this study investigated the effect of iron overload on mouse embryos and elucidated the molecular mechanism. Iron overload in endometriosis PF disrupted blastocyst formation, decreased GPX4 expression and induced lipid peroxidation, suggesting that iron overload causes embryotoxicity and induces ferroptosis. Moreover, mitochondrial damage occurs in iron overload-treated embryos, presenting as decreased ATP levels, increased ROS levels and MMP hyperpolarization. The cytotoxicity of iron overload is attenuated by the ferroptosis inhibitor Fer-1. Furthermore, Smart-seq analysis revealed that HMOX1 is upregulated in embryo ferroptosis and that HMOX1 suppresses ferroptosis by maintaining mitochondrial function. This study provides new insight into the mechanism of endometriosis infertility and a potential target for future endometriosis infertility treatment efforts.Shishi LiYier ZhouQiongxiao HuangXiaohua FuLing ZhangFang GaoZhen JinLimei WuChongyi ShuXirong ZhangWeihai XuJing ShuNature Publishing GrouparticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282CytologyQH573-671ENCell Death Discovery, Vol 7, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671 Shishi Li Yier Zhou Qiongxiao Huang Xiaohua Fu Ling Zhang Fang Gao Zhen Jin Limei Wu Chongyi Shu Xirong Zhang Weihai Xu Jing Shu Iron overload in endometriosis peritoneal fluid induces early embryo ferroptosis mediated by HMOX1
description	Abstract Endometriosis is one of the most common disorders that causes infertility in women. Iron is overloaded in endometriosis peritoneal fluid (PF), with harmful effects on early embryo development. However, the mechanism by which endometriosis peritoneal fluid affects embryonic development remains unclear. Hence, this study investigated the effect of iron overload on mouse embryos and elucidated the molecular mechanism. Iron overload in endometriosis PF disrupted blastocyst formation, decreased GPX4 expression and induced lipid peroxidation, suggesting that iron overload causes embryotoxicity and induces ferroptosis. Moreover, mitochondrial damage occurs in iron overload-treated embryos, presenting as decreased ATP levels, increased ROS levels and MMP hyperpolarization. The cytotoxicity of iron overload is attenuated by the ferroptosis inhibitor Fer-1. Furthermore, Smart-seq analysis revealed that HMOX1 is upregulated in embryo ferroptosis and that HMOX1 suppresses ferroptosis by maintaining mitochondrial function. This study provides new insight into the mechanism of endometriosis infertility and a potential target for future endometriosis infertility treatment efforts.
format	article
author	Shishi Li Yier Zhou Qiongxiao Huang Xiaohua Fu Ling Zhang Fang Gao Zhen Jin Limei Wu Chongyi Shu Xirong Zhang Weihai Xu Jing Shu
author_facet	Shishi Li Yier Zhou Qiongxiao Huang Xiaohua Fu Ling Zhang Fang Gao Zhen Jin Limei Wu Chongyi Shu Xirong Zhang Weihai Xu Jing Shu
author_sort	Shishi Li
title	Iron overload in endometriosis peritoneal fluid induces early embryo ferroptosis mediated by HMOX1
title_short	Iron overload in endometriosis peritoneal fluid induces early embryo ferroptosis mediated by HMOX1
title_full	Iron overload in endometriosis peritoneal fluid induces early embryo ferroptosis mediated by HMOX1
title_fullStr	Iron overload in endometriosis peritoneal fluid induces early embryo ferroptosis mediated by HMOX1
title_full_unstemmed	Iron overload in endometriosis peritoneal fluid induces early embryo ferroptosis mediated by HMOX1
title_sort	iron overload in endometriosis peritoneal fluid induces early embryo ferroptosis mediated by hmox1
publisher	Nature Publishing Group
publishDate	2021
url	https://doaj.org/article/e08c5e22276946269adac69ff1266fa7
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_version_	1718419215444082688

Iron overload in endometriosis peritoneal fluid induces early embryo ferroptosis mediated by HMOX1

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