lncRNA DANCR promotes the migration an invasion and of trophoblast cells through microRNA-214-5p in preeclampsia

Studies have shown that lncRNA DANCR is down-regulated in placental tissues of patients with preeclampsia (PE). The aim of this study was to explore the effect of lncRNA DANCR on trophoblast cells as well as its acting mechanism. We disrupted or overexpressed lncRNA DANCR in trophoblast cells HTR-8/...

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Autores principales:	Qian Zhang, Zhenzhen Wang, Xianghong Cheng, Haiying Wu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	chorionic trophoblast cells mir-214-5p epithelial-mesenchymal transition lncrna dancr preeclampsia Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/e08e0ebf124a4411833711db48f6e36d
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spelling	oai:doaj.org-article:e08e0ebf124a4411833711db48f6e36d2021-12-01T14:41:00ZlncRNA DANCR promotes the migration an invasion and of trophoblast cells through microRNA-214-5p in preeclampsia2165-59792165-598710.1080/21655979.2021.1988373https://doaj.org/article/e08e0ebf124a4411833711db48f6e36d2021-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1988373https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Studies have shown that lncRNA DANCR is down-regulated in placental tissues of patients with preeclampsia (PE). The aim of this study was to explore the effect of lncRNA DANCR on trophoblast cells as well as its acting mechanism. We disrupted or overexpressed lncRNA DANCR in trophoblast cells HTR-8/SVneo and JEG-3 and detected the associated cellular functional changes by MTT, flow cytometry, Transwell experiment, and scratch experiment. The results showed that overexpression of lncRNA DANCR significantly increased the proliferation, invasion, migration, and EMT process of trophoblast cells. Interfering with lncRNA DANCR showed the opposite result. Further, the targeted interaction between lncRNA DANCR and miR-214-5p was confirmed by the dual-luciferase reporter gene assay. In addition, the expression of PI3K/AKT signaling pathway-related proteins was analyzed by Western blot. Overexpression of lncRNA DANCR can increase the phosphorylation of PI3K/AKT protein and activate this signaling pathway. In conclusion, the enforcing of lncRNA DANCR activates the activation of the PI3K/AKT pathway by down-regulating miR-214-5p, and promotes the migration and invasion of chorionic trophoblast cells. This provides a potential new target for PE therapy.Qian ZhangZhenzhen WangXianghong ChengHaiying WuTaylor & Francis Grouparticlechorionic trophoblast cellsmir-214-5pepithelial-mesenchymal transitionlncrna dancrpreeclampsiaBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 2, Pp 9424-9434 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chorionic trophoblast cells mir-214-5p epithelial-mesenchymal transition lncrna dancr preeclampsia Biotechnology TP248.13-248.65
spellingShingle	chorionic trophoblast cells mir-214-5p epithelial-mesenchymal transition lncrna dancr preeclampsia Biotechnology TP248.13-248.65 Qian Zhang Zhenzhen Wang Xianghong Cheng Haiying Wu lncRNA DANCR promotes the migration an invasion and of trophoblast cells through microRNA-214-5p in preeclampsia
description	Studies have shown that lncRNA DANCR is down-regulated in placental tissues of patients with preeclampsia (PE). The aim of this study was to explore the effect of lncRNA DANCR on trophoblast cells as well as its acting mechanism. We disrupted or overexpressed lncRNA DANCR in trophoblast cells HTR-8/SVneo and JEG-3 and detected the associated cellular functional changes by MTT, flow cytometry, Transwell experiment, and scratch experiment. The results showed that overexpression of lncRNA DANCR significantly increased the proliferation, invasion, migration, and EMT process of trophoblast cells. Interfering with lncRNA DANCR showed the opposite result. Further, the targeted interaction between lncRNA DANCR and miR-214-5p was confirmed by the dual-luciferase reporter gene assay. In addition, the expression of PI3K/AKT signaling pathway-related proteins was analyzed by Western blot. Overexpression of lncRNA DANCR can increase the phosphorylation of PI3K/AKT protein and activate this signaling pathway. In conclusion, the enforcing of lncRNA DANCR activates the activation of the PI3K/AKT pathway by down-regulating miR-214-5p, and promotes the migration and invasion of chorionic trophoblast cells. This provides a potential new target for PE therapy.
format	article
author	Qian Zhang Zhenzhen Wang Xianghong Cheng Haiying Wu
author_facet	Qian Zhang Zhenzhen Wang Xianghong Cheng Haiying Wu
author_sort	Qian Zhang
title	lncRNA DANCR promotes the migration an invasion and of trophoblast cells through microRNA-214-5p in preeclampsia
title_short	lncRNA DANCR promotes the migration an invasion and of trophoblast cells through microRNA-214-5p in preeclampsia
title_full	lncRNA DANCR promotes the migration an invasion and of trophoblast cells through microRNA-214-5p in preeclampsia
title_fullStr	lncRNA DANCR promotes the migration an invasion and of trophoblast cells through microRNA-214-5p in preeclampsia
title_full_unstemmed	lncRNA DANCR promotes the migration an invasion and of trophoblast cells through microRNA-214-5p in preeclampsia
title_sort	lncrna dancr promotes the migration an invasion and of trophoblast cells through microrna-214-5p in preeclampsia
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/e08e0ebf124a4411833711db48f6e36d
work_keys_str_mv	AT qianzhang lncrnadancrpromotesthemigrationaninvasionandoftrophoblastcellsthroughmicrorna2145pinpreeclampsia AT zhenzhenwang lncrnadancrpromotesthemigrationaninvasionandoftrophoblastcellsthroughmicrorna2145pinpreeclampsia AT xianghongcheng lncrnadancrpromotesthemigrationaninvasionandoftrophoblastcellsthroughmicrorna2145pinpreeclampsia AT haiyingwu lncrnadancrpromotesthemigrationaninvasionandoftrophoblastcellsthroughmicrorna2145pinpreeclampsia
_version_	1718405027040591872

lncRNA DANCR promotes the migration an invasion and of trophoblast cells through microRNA-214-5p in preeclampsia

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