Fabrication of cartilage/natural rubber latex biocomposites derived from human mesenchymal stem cells in hypoxia
To broaden the knowledge regarding regenerative medicine in natural rubber latex (NRL) nanoparticles, we have examined the chondrogenesis of human mesenchymal stem cells (hMSCs) with NRL nanoparticles under hypoxia condition. We have successfully fabricated the cartilage/NRL biocomposites via hMSCs...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Taylor & Francis Group
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/e0958055af284dde9c658a80858cd385 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:e0958055af284dde9c658a80858cd385 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:e0958055af284dde9c658a80858cd3852021-12-02T17:27:58ZFabrication of cartilage/natural rubber latex biocomposites derived from human mesenchymal stem cells in hypoxia2055-033210.1080/20550324.2020.1851438https://doaj.org/article/e0958055af284dde9c658a80858cd3852020-11-01T00:00:00Zhttp://dx.doi.org/10.1080/20550324.2020.1851438https://doaj.org/toc/2055-0332To broaden the knowledge regarding regenerative medicine in natural rubber latex (NRL) nanoparticles, we have examined the chondrogenesis of human mesenchymal stem cells (hMSCs) with NRL nanoparticles under hypoxia condition. We have successfully fabricated the cartilage/NRL biocomposites via hMSCs spheroid under hypoxic condition, where the administration of NRL nanoparticles exhibits the suppression of the spheroid contraction due to the cellular proliferation of hMSCs. It has been found that the NRL nanoparticles acted as a main component, which provides surface heterogeneity of the spheroid, leading to the mechanically stable structure with higher modulus in comparison with the control as revealed by atomic force microscopy microindentation. Under hypoxia condition, the effective gene expression of in vitro chondrogenesis of hMSCs with administration of NRL was confirmed as revealed by the chondrogenic gene expression analysis compared with that under normoxia condition.Yuki OkamotoMasaya KinoshitaMasami OkamotoTaylor & Francis Grouparticlenatural rubber latexhmscbiocompositeschondrogenesisspheroidmechanical propertiesmicroindentationnormoxiaMaterials of engineering and construction. Mechanics of materialsTA401-492Polymers and polymer manufactureTP1080-1185ENNanocomposites, Vol 6, Iss 4, Pp 137-148 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
natural rubber latex hmsc biocomposites chondrogenesis spheroid mechanical properties microindentation normoxia Materials of engineering and construction. Mechanics of materials TA401-492 Polymers and polymer manufacture TP1080-1185 |
| spellingShingle |
natural rubber latex hmsc biocomposites chondrogenesis spheroid mechanical properties microindentation normoxia Materials of engineering and construction. Mechanics of materials TA401-492 Polymers and polymer manufacture TP1080-1185 Yuki Okamoto Masaya Kinoshita Masami Okamoto Fabrication of cartilage/natural rubber latex biocomposites derived from human mesenchymal stem cells in hypoxia |
| description |
To broaden the knowledge regarding regenerative medicine in natural rubber latex (NRL) nanoparticles, we have examined the chondrogenesis of human mesenchymal stem cells (hMSCs) with NRL nanoparticles under hypoxia condition. We have successfully fabricated the cartilage/NRL biocomposites via hMSCs spheroid under hypoxic condition, where the administration of NRL nanoparticles exhibits the suppression of the spheroid contraction due to the cellular proliferation of hMSCs. It has been found that the NRL nanoparticles acted as a main component, which provides surface heterogeneity of the spheroid, leading to the mechanically stable structure with higher modulus in comparison with the control as revealed by atomic force microscopy microindentation. Under hypoxia condition, the effective gene expression of in vitro chondrogenesis of hMSCs with administration of NRL was confirmed as revealed by the chondrogenic gene expression analysis compared with that under normoxia condition. |
| format |
article |
| author |
Yuki Okamoto Masaya Kinoshita Masami Okamoto |
| author_facet |
Yuki Okamoto Masaya Kinoshita Masami Okamoto |
| author_sort |
Yuki Okamoto |
| title |
Fabrication of cartilage/natural rubber latex biocomposites derived from human mesenchymal stem cells in hypoxia |
| title_short |
Fabrication of cartilage/natural rubber latex biocomposites derived from human mesenchymal stem cells in hypoxia |
| title_full |
Fabrication of cartilage/natural rubber latex biocomposites derived from human mesenchymal stem cells in hypoxia |
| title_fullStr |
Fabrication of cartilage/natural rubber latex biocomposites derived from human mesenchymal stem cells in hypoxia |
| title_full_unstemmed |
Fabrication of cartilage/natural rubber latex biocomposites derived from human mesenchymal stem cells in hypoxia |
| title_sort |
fabrication of cartilage/natural rubber latex biocomposites derived from human mesenchymal stem cells in hypoxia |
| publisher |
Taylor & Francis Group |
| publishDate |
2020 |
| url |
https://doaj.org/article/e0958055af284dde9c658a80858cd385 |
| work_keys_str_mv |
AT yukiokamoto fabricationofcartilagenaturalrubberlatexbiocompositesderivedfromhumanmesenchymalstemcellsinhypoxia AT masayakinoshita fabricationofcartilagenaturalrubberlatexbiocompositesderivedfromhumanmesenchymalstemcellsinhypoxia AT masamiokamoto fabricationofcartilagenaturalrubberlatexbiocompositesderivedfromhumanmesenchymalstemcellsinhypoxia |
| _version_ |
1718380759839932416 |