Effects of human serum albumin on post-mortem changes of malathion

Abstract Malathion, diethyl 2-[(dimethoxyphosphinothioyl)thio]butanedioate, is one of most widely used organophosphoryl pesticide, and it has been detected in several clinical cases of accidental exposure and suicide. It is reported that the observed malathion concentration in blood of persons who s...

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Autores principales: Yoshikazu Yamagishi, Hirotaro Iwase, Yasumitsu Ogra
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/e0a8e6e17107497babb82e326139cf03
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spelling oai:doaj.org-article:e0a8e6e17107497babb82e326139cf032021-12-02T15:56:41ZEffects of human serum albumin on post-mortem changes of malathion10.1038/s41598-021-91145-y2045-2322https://doaj.org/article/e0a8e6e17107497babb82e326139cf032021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91145-yhttps://doaj.org/toc/2045-2322Abstract Malathion, diethyl 2-[(dimethoxyphosphinothioyl)thio]butanedioate, is one of most widely used organophosphoryl pesticide, and it has been detected in several clinical cases of accidental exposure and suicide. It is reported that the observed malathion concentration in blood of persons who suffer from malathion poisoning is smaller than the expected concentration. Because malathion is bound to human serum albumin (HSA), recovery of malathion in the free form is insufficient. We detected malathion adducts in HSA by liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q/TOF–MS). The mass spectra showed that malathion was preferably bound to the lysine (K) and cysteinylproline (CP) residues of HSA. The K- and CP-adducts of malathion were increased in vitro with a dose-dependent fashion when its concentration was smaller than the lethal dose. Further, the K-adduct was also detected in post-mortem blood of an autopsied subject suffering from intentional malathion ingestion. These results suggest that the K-adduct seems to be available to use a biomarker of malathion poisoning, and the determination of the K-adduct could make possible to estimate the amount of malathion ingestion.Yoshikazu YamagishiHirotaro IwaseYasumitsu OgraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Yoshikazu Yamagishi
Hirotaro Iwase
Yasumitsu Ogra
Effects of human serum albumin on post-mortem changes of malathion
description Abstract Malathion, diethyl 2-[(dimethoxyphosphinothioyl)thio]butanedioate, is one of most widely used organophosphoryl pesticide, and it has been detected in several clinical cases of accidental exposure and suicide. It is reported that the observed malathion concentration in blood of persons who suffer from malathion poisoning is smaller than the expected concentration. Because malathion is bound to human serum albumin (HSA), recovery of malathion in the free form is insufficient. We detected malathion adducts in HSA by liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q/TOF–MS). The mass spectra showed that malathion was preferably bound to the lysine (K) and cysteinylproline (CP) residues of HSA. The K- and CP-adducts of malathion were increased in vitro with a dose-dependent fashion when its concentration was smaller than the lethal dose. Further, the K-adduct was also detected in post-mortem blood of an autopsied subject suffering from intentional malathion ingestion. These results suggest that the K-adduct seems to be available to use a biomarker of malathion poisoning, and the determination of the K-adduct could make possible to estimate the amount of malathion ingestion.
format article
author Yoshikazu Yamagishi
Hirotaro Iwase
Yasumitsu Ogra
author_facet Yoshikazu Yamagishi
Hirotaro Iwase
Yasumitsu Ogra
author_sort Yoshikazu Yamagishi
title Effects of human serum albumin on post-mortem changes of malathion
title_short Effects of human serum albumin on post-mortem changes of malathion
title_full Effects of human serum albumin on post-mortem changes of malathion
title_fullStr Effects of human serum albumin on post-mortem changes of malathion
title_full_unstemmed Effects of human serum albumin on post-mortem changes of malathion
title_sort effects of human serum albumin on post-mortem changes of malathion
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/e0a8e6e17107497babb82e326139cf03
work_keys_str_mv AT yoshikazuyamagishi effectsofhumanserumalbuminonpostmortemchangesofmalathion
AT hirotaroiwase effectsofhumanserumalbuminonpostmortemchangesofmalathion
AT yasumitsuogra effectsofhumanserumalbuminonpostmortemchangesofmalathion
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