Effects of human serum albumin on post-mortem changes of malathion

Abstract Malathion, diethyl 2-[(dimethoxyphosphinothioyl)thio]butanedioate, is one of most widely used organophosphoryl pesticide, and it has been detected in several clinical cases of accidental exposure and suicide. It is reported that the observed malathion concentration in blood of persons who s...

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Autores principales:	Yoshikazu Yamagishi, Hirotaro Iwase, Yasumitsu Ogra
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e0a8e6e17107497babb82e326139cf03
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spelling	oai:doaj.org-article:e0a8e6e17107497babb82e326139cf032021-12-02T15:56:41ZEffects of human serum albumin on post-mortem changes of malathion10.1038/s41598-021-91145-y2045-2322https://doaj.org/article/e0a8e6e17107497babb82e326139cf032021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91145-yhttps://doaj.org/toc/2045-2322Abstract Malathion, diethyl 2-[(dimethoxyphosphinothioyl)thio]butanedioate, is one of most widely used organophosphoryl pesticide, and it has been detected in several clinical cases of accidental exposure and suicide. It is reported that the observed malathion concentration in blood of persons who suffer from malathion poisoning is smaller than the expected concentration. Because malathion is bound to human serum albumin (HSA), recovery of malathion in the free form is insufficient. We detected malathion adducts in HSA by liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q/TOF–MS). The mass spectra showed that malathion was preferably bound to the lysine (K) and cysteinylproline (CP) residues of HSA. The K- and CP-adducts of malathion were increased in vitro with a dose-dependent fashion when its concentration was smaller than the lethal dose. Further, the K-adduct was also detected in post-mortem blood of an autopsied subject suffering from intentional malathion ingestion. These results suggest that the K-adduct seems to be available to use a biomarker of malathion poisoning, and the determination of the K-adduct could make possible to estimate the amount of malathion ingestion.Yoshikazu YamagishiHirotaro IwaseYasumitsu OgraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoshikazu Yamagishi Hirotaro Iwase Yasumitsu Ogra Effects of human serum albumin on post-mortem changes of malathion
description	Abstract Malathion, diethyl 2-[(dimethoxyphosphinothioyl)thio]butanedioate, is one of most widely used organophosphoryl pesticide, and it has been detected in several clinical cases of accidental exposure and suicide. It is reported that the observed malathion concentration in blood of persons who suffer from malathion poisoning is smaller than the expected concentration. Because malathion is bound to human serum albumin (HSA), recovery of malathion in the free form is insufficient. We detected malathion adducts in HSA by liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q/TOF–MS). The mass spectra showed that malathion was preferably bound to the lysine (K) and cysteinylproline (CP) residues of HSA. The K- and CP-adducts of malathion were increased in vitro with a dose-dependent fashion when its concentration was smaller than the lethal dose. Further, the K-adduct was also detected in post-mortem blood of an autopsied subject suffering from intentional malathion ingestion. These results suggest that the K-adduct seems to be available to use a biomarker of malathion poisoning, and the determination of the K-adduct could make possible to estimate the amount of malathion ingestion.
format	article
author	Yoshikazu Yamagishi Hirotaro Iwase Yasumitsu Ogra
author_facet	Yoshikazu Yamagishi Hirotaro Iwase Yasumitsu Ogra
author_sort	Yoshikazu Yamagishi
title	Effects of human serum albumin on post-mortem changes of malathion
title_short	Effects of human serum albumin on post-mortem changes of malathion
title_full	Effects of human serum albumin on post-mortem changes of malathion
title_fullStr	Effects of human serum albumin on post-mortem changes of malathion
title_full_unstemmed	Effects of human serum albumin on post-mortem changes of malathion
title_sort	effects of human serum albumin on post-mortem changes of malathion
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e0a8e6e17107497babb82e326139cf03
work_keys_str_mv	AT yoshikazuyamagishi effectsofhumanserumalbuminonpostmortemchangesofmalathion AT hirotaroiwase effectsofhumanserumalbuminonpostmortemchangesofmalathion AT yasumitsuogra effectsofhumanserumalbuminonpostmortemchangesofmalathion
_version_	1718385421841334272

Effects of human serum albumin on post-mortem changes of malathion

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