Donor age-related biological properties of human dental pulp stem cells change in nanostructured scaffolds.

The aim of the present work is to study how biological properties, such as proliferation and commitment ability, of human adult dental pulp stem cells (DPSCs) relate to the age of the donor. Human dental pulps were extracted from molars of healthy adult subjects aged 16 to >66 years. DPSCs were i...

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Autores principales:	Eriberto Bressan, Letizia Ferroni, Chiara Gardin, Paolo Pinton, Edoardo Stellini, Daniele Botticelli, Stefano Sivolella, Barbara Zavan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e0ad570f87884e979f6a1dcbaeb21070
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spelling	oai:doaj.org-article:e0ad570f87884e979f6a1dcbaeb210702021-11-18T08:07:19ZDonor age-related biological properties of human dental pulp stem cells change in nanostructured scaffolds.1932-620310.1371/journal.pone.0049146https://doaj.org/article/e0ad570f87884e979f6a1dcbaeb210702012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23209565/?tool=EBIhttps://doaj.org/toc/1932-6203The aim of the present work is to study how biological properties, such as proliferation and commitment ability, of human adult dental pulp stem cells (DPSCs) relate to the age of the donor. Human dental pulps were extracted from molars of healthy adult subjects aged 16 to >66 years. DPSCs were isolated and cultured in the presence of osteogenic, neurogenic, or vasculogenic differentiation medium. Proliferation ability was evaluated by determining doubling time, and commitment ability was evaluated by gene expression and morphological analyses for tissue-specific markers. The results confirm a well-defined proliferative ability for each donor age group at an early in vitro passage (p2). DPSCs from younger donors (up to 35 years) maintain this ability in long-term cultures (p8). Stem cells of all age donor groups maintain their commitment ability during in vitro culture. In vivo tests on the critical size defect repair process confirmed that DPSCs of all donor ages are a potent tool for bone tissue regeneration when mixed with 3D nanostructured scaffolds.Eriberto BressanLetizia FerroniChiara GardinPaolo PintonEdoardo StelliniDaniele BotticelliStefano SivolellaBarbara ZavanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e49146 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eriberto Bressan Letizia Ferroni Chiara Gardin Paolo Pinton Edoardo Stellini Daniele Botticelli Stefano Sivolella Barbara Zavan Donor age-related biological properties of human dental pulp stem cells change in nanostructured scaffolds.
description	The aim of the present work is to study how biological properties, such as proliferation and commitment ability, of human adult dental pulp stem cells (DPSCs) relate to the age of the donor. Human dental pulps were extracted from molars of healthy adult subjects aged 16 to >66 years. DPSCs were isolated and cultured in the presence of osteogenic, neurogenic, or vasculogenic differentiation medium. Proliferation ability was evaluated by determining doubling time, and commitment ability was evaluated by gene expression and morphological analyses for tissue-specific markers. The results confirm a well-defined proliferative ability for each donor age group at an early in vitro passage (p2). DPSCs from younger donors (up to 35 years) maintain this ability in long-term cultures (p8). Stem cells of all age donor groups maintain their commitment ability during in vitro culture. In vivo tests on the critical size defect repair process confirmed that DPSCs of all donor ages are a potent tool for bone tissue regeneration when mixed with 3D nanostructured scaffolds.
format	article
author	Eriberto Bressan Letizia Ferroni Chiara Gardin Paolo Pinton Edoardo Stellini Daniele Botticelli Stefano Sivolella Barbara Zavan
author_facet	Eriberto Bressan Letizia Ferroni Chiara Gardin Paolo Pinton Edoardo Stellini Daniele Botticelli Stefano Sivolella Barbara Zavan
author_sort	Eriberto Bressan
title	Donor age-related biological properties of human dental pulp stem cells change in nanostructured scaffolds.
title_short	Donor age-related biological properties of human dental pulp stem cells change in nanostructured scaffolds.
title_full	Donor age-related biological properties of human dental pulp stem cells change in nanostructured scaffolds.
title_fullStr	Donor age-related biological properties of human dental pulp stem cells change in nanostructured scaffolds.
title_full_unstemmed	Donor age-related biological properties of human dental pulp stem cells change in nanostructured scaffolds.
title_sort	donor age-related biological properties of human dental pulp stem cells change in nanostructured scaffolds.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e0ad570f87884e979f6a1dcbaeb21070
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Donor age-related biological properties of human dental pulp stem cells change in nanostructured scaffolds.

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