Early exposure to distinct sources of lipids affects differently the development and hepatic inflammatory profiles of 21-day-old rat offspring

Laís Vales Mennitti,1 Lila Missae Oyama,2 Aline Boveto Santamarina,1 Claudia Maria da Penha Oller do Nascimento,2 Luciana Pellegrini Pisani3 1PhD Program ‘Interdisciplinar in Health Sciences’, Federal University of São Paulo (UNIFESP), Santos, SP, B...

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Autores principales:	Mennitti LV, Oyama LM, Santamarina AB, do Nascimento CM, Pisani LP
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Fatty acids Pregnancy and Lactation Adiposity Pro-inflammatory status Offspring Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/e0c915281617480c8a950b05a2fc39bb
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spelling	oai:doaj.org-article:e0c915281617480c8a950b05a2fc39bb2021-12-02T02:11:47ZEarly exposure to distinct sources of lipids affects differently the development and hepatic inflammatory profiles of 21-day-old rat offspring1178-7031https://doaj.org/article/e0c915281617480c8a950b05a2fc39bb2018-01-01T00:00:00Zhttps://www.dovepress.com/early-exposure-to-distinct-sources-of-lipids-affects-differently-the-d-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Laís Vales Mennitti,1 Lila Missae Oyama,2 Aline Boveto Santamarina,1 Claudia Maria da Penha Oller do Nascimento,2 Luciana Pellegrini Pisani3 1PhD Program ‘Interdisciplinar in Health Sciences’, Federal University of São Paulo (UNIFESP), Santos, SP, Brazil; 2Department of Physiology, Discipline of Nutrition Physiology, Federal University of São Paulo (UNIFESP), São Paulo, SP, Brazil; 3Department of Biosciences, Institute of Health and Society, Federal University of São Paulo (UNIFESP), Santos, SP, Brazil Introduction: Maternal diet composition of fatty acids during pregnancy and lactation seems to modify the fetal programming, epigenetic pattern and offspring phenotype. Aim: Herein, we investigated the effects of maternal consumption of normal-fat diets with distinct lipid sources during pregnancy and lactation on the somatic development and proinflammatory status of 21-day-old rat offspring. Materials and Methods: On the first day of pregnancy, female Wistar rats were divided into four groups as follows: soybean oil (M-SO), lard (M-L), hydrogenated vegetable fat (M-HVF) and fish oil (M-FO). Diets were maintained during pregnancy and lactation. Male offspring constituted the SO, L, HVF and FO groups. Pups were weighed and measured weekly. Lipopolysaccharide serum concentration was determined. Tumor necrosis factor alpha, interleukin (IL)-6 and IL-10 in the liver were evaluated by enzyme-linked immunosorbent assay. Liver gene expressions were determined by real-time polymerase chain reaction. Protein expressions in the liver were analyzed by Western blotting. Results: We observed an increase in body weight and adiposity in L and HVF groups. Moreover, HVF group showed an increase in the toll-like receptor 4 mRNA levels, IL10Rα and phosphorylated form of IκB kinase (IKK; p-IKKα+β) protein expression. The FO group presented a decrease in body weight, relative weight of retroperitoneal adipose tissue, ADIPOR2 gene expression, lipopolysaccharide and p-IKKα+β and phosphorylated form of nuclear transcription factor kappa B (NFκB) p50 (p-NFκB p50) protein expression. Conclusion: Summarily, whereas maternal intake of normal-fat diets based on L and HVF appear to affect the somatic development negatively, only early exposure to HVF impairs the pups’ proinflammatory status. In contrast, maternal diets based on FO during pregnancy and lactation have been more beneficial to the adiposity and toll-like receptor 4 signaling pathway of the 21-day-old rat offspring, particularly when compared to L or HVF diets. Keywords: fatty acids, pregnancy and lactation, adiposity, proinflammatory status, pupsMennitti LVOyama LMSantamarina ABdo Nascimento CMPisani LPDove Medical PressarticleFatty acidsPregnancy and LactationAdiposityPro-inflammatory statusOffspringPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 11, Pp 11-24 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Fatty acids Pregnancy and Lactation Adiposity Pro-inflammatory status Offspring Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	Fatty acids Pregnancy and Lactation Adiposity Pro-inflammatory status Offspring Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Mennitti LV Oyama LM Santamarina AB do Nascimento CM Pisani LP Early exposure to distinct sources of lipids affects differently the development and hepatic inflammatory profiles of 21-day-old rat offspring
description	Laís Vales Mennitti,1 Lila Missae Oyama,2 Aline Boveto Santamarina,1 Claudia Maria da Penha Oller do Nascimento,2 Luciana Pellegrini Pisani3 1PhD Program ‘Interdisciplinar in Health Sciences’, Federal University of São Paulo (UNIFESP), Santos, SP, Brazil; 2Department of Physiology, Discipline of Nutrition Physiology, Federal University of São Paulo (UNIFESP), São Paulo, SP, Brazil; 3Department of Biosciences, Institute of Health and Society, Federal University of São Paulo (UNIFESP), Santos, SP, Brazil Introduction: Maternal diet composition of fatty acids during pregnancy and lactation seems to modify the fetal programming, epigenetic pattern and offspring phenotype. Aim: Herein, we investigated the effects of maternal consumption of normal-fat diets with distinct lipid sources during pregnancy and lactation on the somatic development and proinflammatory status of 21-day-old rat offspring. Materials and Methods: On the first day of pregnancy, female Wistar rats were divided into four groups as follows: soybean oil (M-SO), lard (M-L), hydrogenated vegetable fat (M-HVF) and fish oil (M-FO). Diets were maintained during pregnancy and lactation. Male offspring constituted the SO, L, HVF and FO groups. Pups were weighed and measured weekly. Lipopolysaccharide serum concentration was determined. Tumor necrosis factor alpha, interleukin (IL)-6 and IL-10 in the liver were evaluated by enzyme-linked immunosorbent assay. Liver gene expressions were determined by real-time polymerase chain reaction. Protein expressions in the liver were analyzed by Western blotting. Results: We observed an increase in body weight and adiposity in L and HVF groups. Moreover, HVF group showed an increase in the toll-like receptor 4 mRNA levels, IL10Rα and phosphorylated form of IκB kinase (IKK; p-IKKα+β) protein expression. The FO group presented a decrease in body weight, relative weight of retroperitoneal adipose tissue, ADIPOR2 gene expression, lipopolysaccharide and p-IKKα+β and phosphorylated form of nuclear transcription factor kappa B (NFκB) p50 (p-NFκB p50) protein expression. Conclusion: Summarily, whereas maternal intake of normal-fat diets based on L and HVF appear to affect the somatic development negatively, only early exposure to HVF impairs the pups’ proinflammatory status. In contrast, maternal diets based on FO during pregnancy and lactation have been more beneficial to the adiposity and toll-like receptor 4 signaling pathway of the 21-day-old rat offspring, particularly when compared to L or HVF diets. Keywords: fatty acids, pregnancy and lactation, adiposity, proinflammatory status, pups
format	article
author	Mennitti LV Oyama LM Santamarina AB do Nascimento CM Pisani LP
author_facet	Mennitti LV Oyama LM Santamarina AB do Nascimento CM Pisani LP
author_sort	Mennitti LV
title	Early exposure to distinct sources of lipids affects differently the development and hepatic inflammatory profiles of 21-day-old rat offspring
title_short	Early exposure to distinct sources of lipids affects differently the development and hepatic inflammatory profiles of 21-day-old rat offspring
title_full	Early exposure to distinct sources of lipids affects differently the development and hepatic inflammatory profiles of 21-day-old rat offspring
title_fullStr	Early exposure to distinct sources of lipids affects differently the development and hepatic inflammatory profiles of 21-day-old rat offspring
title_full_unstemmed	Early exposure to distinct sources of lipids affects differently the development and hepatic inflammatory profiles of 21-day-old rat offspring
title_sort	early exposure to distinct sources of lipids affects differently the development and hepatic inflammatory profiles of 21-day-old rat offspring
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/e0c915281617480c8a950b05a2fc39bb
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Early exposure to distinct sources of lipids affects differently the development and hepatic inflammatory profiles of 21-day-old rat offspring

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