Antimicrobial Peptides With Antibiofilm Activity Against Xylella fastidiosa

Xylella fastidiosa is a plant pathogen that was recently introduced in Europe and is causing havoc to its agriculture. This Gram-negative bacterium invades the host xylem, multiplies, and forms biofilm occluding the vessels and killing its host. In spite of the great research effort, there is no met...

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Autores principales:	Luís Moll, Esther Badosa, Marta Planas, Lidia Feliu, Emilio Montesinos, Anna Bonaterra
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Xylella fastidiosa bactericidal peptides antibiofilm peptides biofilm production planktonic cells Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e0c9ab32771943e69a99f36711d5e4fe
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spelling	oai:doaj.org-article:e0c9ab32771943e69a99f36711d5e4fe2021-11-08T07:25:21ZAntimicrobial Peptides With Antibiofilm Activity Against Xylella fastidiosa1664-302X10.3389/fmicb.2021.753874https://doaj.org/article/e0c9ab32771943e69a99f36711d5e4fe2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.753874/fullhttps://doaj.org/toc/1664-302XXylella fastidiosa is a plant pathogen that was recently introduced in Europe and is causing havoc to its agriculture. This Gram-negative bacterium invades the host xylem, multiplies, and forms biofilm occluding the vessels and killing its host. In spite of the great research effort, there is no method that effectively prevents or cures hosts from infections. The main control strategies up to now are eradication, vector control, and pathogen-free plant material. Antimicrobial peptides have arisen as promising candidates to combat this bacterium due to their broad spectrum of activity and low environmental impact. In this work, peptides previously reported in the literature and newly designed analogs were studied for its bactericidal and antibiofilm activity against X. fastidiosa. Also, their hemolytic activity and effect on tobacco leaves when infiltrated were determined. To assess the activity of peptides, the strain IVIA 5387.2 with moderate growth, able to produce biofilm and susceptible to antimicrobial peptides, was selected among six representative strains found in the Mediterranean area (DD1, CFBP 8173, Temecula, IVIA 5387.2, IVIA 5770, and IVIA 5901.2). Two interesting groups of peptides were identified with bactericidal and/or antibiofilm activity and low-moderate toxicity. The peptides 1036 and RIJK2 with dual (bactericidal–antibiofilm) activity against the pathogen and moderate toxicity stand out as the best candidates to control X. fastidiosa diseases. Nevertheless, peptides with only antibiofilm activity and low toxicity are also promising agents as they could prevent the occlusion of xylem vessels caused by the pathogen. The present work contributes to provide novel compounds with antimicrobial and antibiofilm activity that could lead to the development of new treatments against diseases caused by X. fastidiosa.Luís MollEsther BadosaMarta PlanasLidia FeliuEmilio MontesinosAnna BonaterraFrontiers Media S.A.articleXylella fastidiosabactericidal peptidesantibiofilm peptidesbiofilm productionplanktonic cellsMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Xylella fastidiosa bactericidal peptides antibiofilm peptides biofilm production planktonic cells Microbiology QR1-502
spellingShingle	Xylella fastidiosa bactericidal peptides antibiofilm peptides biofilm production planktonic cells Microbiology QR1-502 Luís Moll Esther Badosa Marta Planas Lidia Feliu Emilio Montesinos Anna Bonaterra Antimicrobial Peptides With Antibiofilm Activity Against Xylella fastidiosa
description	Xylella fastidiosa is a plant pathogen that was recently introduced in Europe and is causing havoc to its agriculture. This Gram-negative bacterium invades the host xylem, multiplies, and forms biofilm occluding the vessels and killing its host. In spite of the great research effort, there is no method that effectively prevents or cures hosts from infections. The main control strategies up to now are eradication, vector control, and pathogen-free plant material. Antimicrobial peptides have arisen as promising candidates to combat this bacterium due to their broad spectrum of activity and low environmental impact. In this work, peptides previously reported in the literature and newly designed analogs were studied for its bactericidal and antibiofilm activity against X. fastidiosa. Also, their hemolytic activity and effect on tobacco leaves when infiltrated were determined. To assess the activity of peptides, the strain IVIA 5387.2 with moderate growth, able to produce biofilm and susceptible to antimicrobial peptides, was selected among six representative strains found in the Mediterranean area (DD1, CFBP 8173, Temecula, IVIA 5387.2, IVIA 5770, and IVIA 5901.2). Two interesting groups of peptides were identified with bactericidal and/or antibiofilm activity and low-moderate toxicity. The peptides 1036 and RIJK2 with dual (bactericidal–antibiofilm) activity against the pathogen and moderate toxicity stand out as the best candidates to control X. fastidiosa diseases. Nevertheless, peptides with only antibiofilm activity and low toxicity are also promising agents as they could prevent the occlusion of xylem vessels caused by the pathogen. The present work contributes to provide novel compounds with antimicrobial and antibiofilm activity that could lead to the development of new treatments against diseases caused by X. fastidiosa.
format	article
author	Luís Moll Esther Badosa Marta Planas Lidia Feliu Emilio Montesinos Anna Bonaterra
author_facet	Luís Moll Esther Badosa Marta Planas Lidia Feliu Emilio Montesinos Anna Bonaterra
author_sort	Luís Moll
title	Antimicrobial Peptides With Antibiofilm Activity Against Xylella fastidiosa
title_short	Antimicrobial Peptides With Antibiofilm Activity Against Xylella fastidiosa
title_full	Antimicrobial Peptides With Antibiofilm Activity Against Xylella fastidiosa
title_fullStr	Antimicrobial Peptides With Antibiofilm Activity Against Xylella fastidiosa
title_full_unstemmed	Antimicrobial Peptides With Antibiofilm Activity Against Xylella fastidiosa
title_sort	antimicrobial peptides with antibiofilm activity against xylella fastidiosa
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e0c9ab32771943e69a99f36711d5e4fe
work_keys_str_mv	AT luismoll antimicrobialpeptideswithantibiofilmactivityagainstxylellafastidiosa AT estherbadosa antimicrobialpeptideswithantibiofilmactivityagainstxylellafastidiosa AT martaplanas antimicrobialpeptideswithantibiofilmactivityagainstxylellafastidiosa AT lidiafeliu antimicrobialpeptideswithantibiofilmactivityagainstxylellafastidiosa AT emiliomontesinos antimicrobialpeptideswithantibiofilmactivityagainstxylellafastidiosa AT annabonaterra antimicrobialpeptideswithantibiofilmactivityagainstxylellafastidiosa
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Antimicrobial Peptides With Antibiofilm Activity Against Xylella fastidiosa

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