Geniposide Balances the Redox Signaling to Mediate Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells
Chunyan Liu, Yanan Hao, Fei Yin, Jianhui Liu Chongqing Key Laboratory of Medicinal Chemistry & Molecular Pharmacology, Chongqing University of Technology, Chongqing 400054, People’s Republic of ChinaCorrespondence: Jianhui Liu; Fei YinChongqing Key Laboratory of Medicinal Chemistry...
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Dove Medical Press
2020
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oai:doaj.org-article:e0cc8a58a8c0423cafd32b5da2bc75892021-12-02T09:08:36ZGeniposide Balances the Redox Signaling to Mediate Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells1178-7007https://doaj.org/article/e0cc8a58a8c0423cafd32b5da2bc75892020-02-01T00:00:00Zhttps://www.dovepress.com/geniposide-balances-the-redox-signaling-to-mediate-glucose-stimulated--peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Chunyan Liu, Yanan Hao, Fei Yin, Jianhui Liu Chongqing Key Laboratory of Medicinal Chemistry & Molecular Pharmacology, Chongqing University of Technology, Chongqing 400054, People’s Republic of ChinaCorrespondence: Jianhui Liu; Fei YinChongqing Key Laboratory of Medicinal Chemistry & Molecular Pharmacology, Chongqing University of Technology, Hongguang Road 69, Ba’nan District, Chongqing 400054, People’s Republic of ChinaTel/Fax +86-23-6256-3182Email jhliu@cqut.edu.cn; fyin@cqut.edu.cnPurpose: To investigate the effect of geniposide on the biosynthesis of insulin and the expression protein disulfide isomerase (PDI) and endoplasmic reticulum oxidoreductin 1 (ERO1) in the presence of low (5 mM) and high (25 mM) glucose in pancreatic β cells.Methods: The content of insulin was measured by ELISA, the number of SH groups was determined with the classical chromogenic reagent, 5,5ʹ-dithiobis-(2-nitrobenzoic) acid (DTNB; also known as Ellman’s reagent), the expressions of PDI and ERO1 were analyzed by Western blot.Results: Geniposide played contrary roles on the accumulation of H 2O 2, the ratio of GSH/GSSG and the thiol–disulfide balance in the presence of low (5 mM) and high (25 mM) glucose in rat pancreatic INS-1 cells. Geniposide also regulated the protein levels of protein disulfide isomerase (PDI) and endoplasmic reticulum oxidoreductin1 (ERO1), the two key enzymes for the production of H 2O 2 during the biosynthesis of insulin in INS-1 cells.Conclusion: Geniposide affects glucose-stimulated insulin secretion by modulating the thiol–disulfide balance that is controlled by the redox signaling in pancreatic β cells.Keywords: endoplasmic reticulum oxidoreductin1, ERO1, geniposide, protein disulfide isomerase, PDI, glucose-stimulated insulin secretion, GSIS, type 2 diabetes mellitus, T2DMLiu CHao YYin FLiu JDove Medical Pressarticleendoplasmic reticulum oxidoreductin1 (ero1)geniposideprotein disulfide isomerase (pdi)glucose-stimulated insulin secretion (gsis)type 2 diabetes mellitus (t2dm)Specialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 509-520 (2020) |
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endoplasmic reticulum oxidoreductin1 (ero1) geniposide protein disulfide isomerase (pdi) glucose-stimulated insulin secretion (gsis) type 2 diabetes mellitus (t2dm) Specialties of internal medicine RC581-951 |
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endoplasmic reticulum oxidoreductin1 (ero1) geniposide protein disulfide isomerase (pdi) glucose-stimulated insulin secretion (gsis) type 2 diabetes mellitus (t2dm) Specialties of internal medicine RC581-951 Liu C Hao Y Yin F Liu J Geniposide Balances the Redox Signaling to Mediate Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells |
| description |
Chunyan Liu, Yanan Hao, Fei Yin, Jianhui Liu Chongqing Key Laboratory of Medicinal Chemistry & Molecular Pharmacology, Chongqing University of Technology, Chongqing 400054, People’s Republic of ChinaCorrespondence: Jianhui Liu; Fei YinChongqing Key Laboratory of Medicinal Chemistry & Molecular Pharmacology, Chongqing University of Technology, Hongguang Road 69, Ba’nan District, Chongqing 400054, People’s Republic of ChinaTel/Fax +86-23-6256-3182Email jhliu@cqut.edu.cn; fyin@cqut.edu.cnPurpose: To investigate the effect of geniposide on the biosynthesis of insulin and the expression protein disulfide isomerase (PDI) and endoplasmic reticulum oxidoreductin 1 (ERO1) in the presence of low (5 mM) and high (25 mM) glucose in pancreatic β cells.Methods: The content of insulin was measured by ELISA, the number of SH groups was determined with the classical chromogenic reagent, 5,5ʹ-dithiobis-(2-nitrobenzoic) acid (DTNB; also known as Ellman’s reagent), the expressions of PDI and ERO1 were analyzed by Western blot.Results: Geniposide played contrary roles on the accumulation of H 2O 2, the ratio of GSH/GSSG and the thiol–disulfide balance in the presence of low (5 mM) and high (25 mM) glucose in rat pancreatic INS-1 cells. Geniposide also regulated the protein levels of protein disulfide isomerase (PDI) and endoplasmic reticulum oxidoreductin1 (ERO1), the two key enzymes for the production of H 2O 2 during the biosynthesis of insulin in INS-1 cells.Conclusion: Geniposide affects glucose-stimulated insulin secretion by modulating the thiol–disulfide balance that is controlled by the redox signaling in pancreatic β cells.Keywords: endoplasmic reticulum oxidoreductin1, ERO1, geniposide, protein disulfide isomerase, PDI, glucose-stimulated insulin secretion, GSIS, type 2 diabetes mellitus, T2DM |
| format |
article |
| author |
Liu C Hao Y Yin F Liu J |
| author_facet |
Liu C Hao Y Yin F Liu J |
| author_sort |
Liu C |
| title |
Geniposide Balances the Redox Signaling to Mediate Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells |
| title_short |
Geniposide Balances the Redox Signaling to Mediate Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells |
| title_full |
Geniposide Balances the Redox Signaling to Mediate Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells |
| title_fullStr |
Geniposide Balances the Redox Signaling to Mediate Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells |
| title_full_unstemmed |
Geniposide Balances the Redox Signaling to Mediate Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells |
| title_sort |
geniposide balances the redox signaling to mediate glucose-stimulated insulin secretion in pancreatic β-cells |
| publisher |
Dove Medical Press |
| publishDate |
2020 |
| url |
https://doaj.org/article/e0cc8a58a8c0423cafd32b5da2bc7589 |
| work_keys_str_mv |
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