Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response.

Infected airway epithelial cells up-regulate the expression of chemokines, chiefly IL-8, and antimicrobial molecules including beta-defensins (BD). Acinetobacter baumannii is a cause of hospital-acquired pneumonia. We examined whether A. baumannii induced the expressions of IL-8 and BD2 by airway ep...

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Autores principales: Catalina March, Verónica Regueiro, Enrique Llobet, David Moranta, Pau Morey, Junkal Garmendia, José A Bengoechea
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Publicado: Public Library of Science (PLoS) 2010
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Acceso en línea:https://doaj.org/article/e0d1fef187c54f84b21e9cbbdb1cab36
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spelling oai:doaj.org-article:e0d1fef187c54f84b21e9cbbdb1cab362021-11-25T06:24:44ZDissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response.1932-620310.1371/journal.pone.0010033https://doaj.org/article/e0d1fef187c54f84b21e9cbbdb1cab362010-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20383325/?tool=EBIhttps://doaj.org/toc/1932-6203Infected airway epithelial cells up-regulate the expression of chemokines, chiefly IL-8, and antimicrobial molecules including beta-defensins (BD). Acinetobacter baumannii is a cause of hospital-acquired pneumonia. We examined whether A. baumannii induced the expressions of IL-8 and BD2 by airway epithelial cells and the receptors implicated in bacterial detection. A549 and human primary airway cells released IL-8 upon infection. A. baumannii-infected cells also increased the expression of BD2 which killed A. baummannii strains. IL-8 induction was via NF-kappaB and mitogen-activated kinases p38 and p44/42-dependent pathways. A. baumannii engaged Toll-like receptor (TLR) 2 and TLR4 pathways and A549 cells could use soluble CD14 as TLRs co-receptor. A. baumannii lipopolysaccharide stimulated IL-8 release by A549 cells and sCD14 facilitated the recognition of the lipopolysaccharide. Mass spectrometry analysis revealed that A. baumannii lipid A structure matches those with endotoxic potential. These results demonstrate that airway epithelial cells produce mediators important for A. baumannii clearance.Catalina MarchVerónica RegueiroEnrique LlobetDavid MorantaPau MoreyJunkal GarmendiaJosé A BengoecheaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 4, p e10033 (2010)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Catalina March
Verónica Regueiro
Enrique Llobet
David Moranta
Pau Morey
Junkal Garmendia
José A Bengoechea
Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response.
description Infected airway epithelial cells up-regulate the expression of chemokines, chiefly IL-8, and antimicrobial molecules including beta-defensins (BD). Acinetobacter baumannii is a cause of hospital-acquired pneumonia. We examined whether A. baumannii induced the expressions of IL-8 and BD2 by airway epithelial cells and the receptors implicated in bacterial detection. A549 and human primary airway cells released IL-8 upon infection. A. baumannii-infected cells also increased the expression of BD2 which killed A. baummannii strains. IL-8 induction was via NF-kappaB and mitogen-activated kinases p38 and p44/42-dependent pathways. A. baumannii engaged Toll-like receptor (TLR) 2 and TLR4 pathways and A549 cells could use soluble CD14 as TLRs co-receptor. A. baumannii lipopolysaccharide stimulated IL-8 release by A549 cells and sCD14 facilitated the recognition of the lipopolysaccharide. Mass spectrometry analysis revealed that A. baumannii lipid A structure matches those with endotoxic potential. These results demonstrate that airway epithelial cells produce mediators important for A. baumannii clearance.
format article
author Catalina March
Verónica Regueiro
Enrique Llobet
David Moranta
Pau Morey
Junkal Garmendia
José A Bengoechea
author_facet Catalina March
Verónica Regueiro
Enrique Llobet
David Moranta
Pau Morey
Junkal Garmendia
José A Bengoechea
author_sort Catalina March
title Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response.
title_short Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response.
title_full Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response.
title_fullStr Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response.
title_full_unstemmed Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response.
title_sort dissection of host cell signal transduction during acinetobacter baumannii-triggered inflammatory response.
publisher Public Library of Science (PLoS)
publishDate 2010
url https://doaj.org/article/e0d1fef187c54f84b21e9cbbdb1cab36
work_keys_str_mv AT catalinamarch dissectionofhostcellsignaltransductionduringacinetobacterbaumanniitriggeredinflammatoryresponse
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