Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response.
Infected airway epithelial cells up-regulate the expression of chemokines, chiefly IL-8, and antimicrobial molecules including beta-defensins (BD). Acinetobacter baumannii is a cause of hospital-acquired pneumonia. We examined whether A. baumannii induced the expressions of IL-8 and BD2 by airway ep...
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2010
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oai:doaj.org-article:e0d1fef187c54f84b21e9cbbdb1cab362021-11-25T06:24:44ZDissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response.1932-620310.1371/journal.pone.0010033https://doaj.org/article/e0d1fef187c54f84b21e9cbbdb1cab362010-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20383325/?tool=EBIhttps://doaj.org/toc/1932-6203Infected airway epithelial cells up-regulate the expression of chemokines, chiefly IL-8, and antimicrobial molecules including beta-defensins (BD). Acinetobacter baumannii is a cause of hospital-acquired pneumonia. We examined whether A. baumannii induced the expressions of IL-8 and BD2 by airway epithelial cells and the receptors implicated in bacterial detection. A549 and human primary airway cells released IL-8 upon infection. A. baumannii-infected cells also increased the expression of BD2 which killed A. baummannii strains. IL-8 induction was via NF-kappaB and mitogen-activated kinases p38 and p44/42-dependent pathways. A. baumannii engaged Toll-like receptor (TLR) 2 and TLR4 pathways and A549 cells could use soluble CD14 as TLRs co-receptor. A. baumannii lipopolysaccharide stimulated IL-8 release by A549 cells and sCD14 facilitated the recognition of the lipopolysaccharide. Mass spectrometry analysis revealed that A. baumannii lipid A structure matches those with endotoxic potential. These results demonstrate that airway epithelial cells produce mediators important for A. baumannii clearance.Catalina MarchVerónica RegueiroEnrique LlobetDavid MorantaPau MoreyJunkal GarmendiaJosé A BengoecheaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 4, p e10033 (2010) |
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DOAJ |
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DOAJ |
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EN |
| topic |
Medicine R Science Q |
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Medicine R Science Q Catalina March Verónica Regueiro Enrique Llobet David Moranta Pau Morey Junkal Garmendia José A Bengoechea Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response. |
| description |
Infected airway epithelial cells up-regulate the expression of chemokines, chiefly IL-8, and antimicrobial molecules including beta-defensins (BD). Acinetobacter baumannii is a cause of hospital-acquired pneumonia. We examined whether A. baumannii induced the expressions of IL-8 and BD2 by airway epithelial cells and the receptors implicated in bacterial detection. A549 and human primary airway cells released IL-8 upon infection. A. baumannii-infected cells also increased the expression of BD2 which killed A. baummannii strains. IL-8 induction was via NF-kappaB and mitogen-activated kinases p38 and p44/42-dependent pathways. A. baumannii engaged Toll-like receptor (TLR) 2 and TLR4 pathways and A549 cells could use soluble CD14 as TLRs co-receptor. A. baumannii lipopolysaccharide stimulated IL-8 release by A549 cells and sCD14 facilitated the recognition of the lipopolysaccharide. Mass spectrometry analysis revealed that A. baumannii lipid A structure matches those with endotoxic potential. These results demonstrate that airway epithelial cells produce mediators important for A. baumannii clearance. |
| format |
article |
| author |
Catalina March Verónica Regueiro Enrique Llobet David Moranta Pau Morey Junkal Garmendia José A Bengoechea |
| author_facet |
Catalina March Verónica Regueiro Enrique Llobet David Moranta Pau Morey Junkal Garmendia José A Bengoechea |
| author_sort |
Catalina March |
| title |
Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response. |
| title_short |
Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response. |
| title_full |
Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response. |
| title_fullStr |
Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response. |
| title_full_unstemmed |
Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response. |
| title_sort |
dissection of host cell signal transduction during acinetobacter baumannii-triggered inflammatory response. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2010 |
| url |
https://doaj.org/article/e0d1fef187c54f84b21e9cbbdb1cab36 |
| work_keys_str_mv |
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| _version_ |
1718413775485272064 |